year 4, Issue 3 (Fall 2010)
Iran J Med Microbiol 2010, 4(3): 53-63 |
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Fazlara A, Maktabi S, Noori A. A survey on enterococci contamination of non-pasteurized ice-creams in Ahvaz using reference and impedance-splitting methods and determining the correlation between the methods. Iran J Med Microbiol 2010; 4 (3) :53-63
URL: http://ijmm.ir/article-1-40-en.html
URL: http://ijmm.ir/article-1-40-en.html
1- Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2- Veterinar
2- Veterinar
Abstract: (19269 Views)
Background and Objectives: Enterococci are known as indicator microorganisms for food safety in many countries. The presence of enterococci in excessive number in foods means undesirable sanitary situation during production and distribution of foods. The aim of this study was to detect enterococcal contamination of non-pasteurized traditional ice-cream during cold and warm seasons with standard reference and impedance-splitting methods and determination the rate of correlation between the two methods.
Material and Methods: 100 samples of non-pasteurized traditional ice-cream during cold and warm seasons were collected from five different areas of Ahvaz and tested for enteroccocci enumeration with both standard reference and impedance-splitting methods. The reference standard method was based on the Standard Institute and Industrial Research (ISIRI) of Iran's recommendation. The impedance method was carried out by measuring the each 10 minutes variation of electrical resistance of selective broth media (M-value) for enteroccocci which used in this method. The rate of concordance and correlation between the two methods was determined using Excel software and the data were analysed using student t-test and Chi squared test.
Results: The result showed that 75 percents of samples in standard method and 79 percent of the samples in impedance-splitting method were contaminated with enteroccocci. Also the minimum and maximum spending time for obtaining results in impedance technique was 6.72 and 22.81 hours respectively which in comparing to conventional standard methods is enormously faster. The rate of correlation between impedance method and the standard reference method in non-pasteurized traditional ice-cream was 87.85 %.
Conclusion: In attention to this fact that impedance-splitting method could examine more samples in shorter time, so this method could be used as a reliable and suitable technique instead of standard conventional methods in quality control of foods.
Material and Methods: 100 samples of non-pasteurized traditional ice-cream during cold and warm seasons were collected from five different areas of Ahvaz and tested for enteroccocci enumeration with both standard reference and impedance-splitting methods. The reference standard method was based on the Standard Institute and Industrial Research (ISIRI) of Iran's recommendation. The impedance method was carried out by measuring the each 10 minutes variation of electrical resistance of selective broth media (M-value) for enteroccocci which used in this method. The rate of concordance and correlation between the two methods was determined using Excel software and the data were analysed using student t-test and Chi squared test.
Results: The result showed that 75 percents of samples in standard method and 79 percent of the samples in impedance-splitting method were contaminated with enteroccocci. Also the minimum and maximum spending time for obtaining results in impedance technique was 6.72 and 22.81 hours respectively which in comparing to conventional standard methods is enormously faster. The rate of correlation between impedance method and the standard reference method in non-pasteurized traditional ice-cream was 87.85 %.
Conclusion: In attention to this fact that impedance-splitting method could examine more samples in shorter time, so this method could be used as a reliable and suitable technique instead of standard conventional methods in quality control of foods.
Type of Study: Original Research Article |
Subject:
Medical Bacteriology
Received: 2013/10/28 | Accepted: 2013/11/10 | ePublished: 2013/11/10
Received: 2013/10/28 | Accepted: 2013/11/10 | ePublished: 2013/11/10
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