year 1, Issue 4 (Winter 2008)
Iran J Med Microbiol 2008, 1(4): 1-11 |
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Feizabadi M, Bahrmand A, Heidarieh P, Shojaee H. Using of multilocus enzyme electrophoresis to study the heterogenicity of clinical isolates of Mycobacterium kansasii. Iran J Med Microbiol 2008; 1 (4) :1-11
URL: http://ijmm.ir/article-1-96-en.html
URL: http://ijmm.ir/article-1-96-en.html
1- Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran , mfeizabadi@tums.ac.ir
2- Departmeno of Mycobacteriology and Lung Diseases, Pasteur Institute of Iran, Tehran
3- Department of Microbiology, Isfahan University of Medical Science, Isfahan
2- Departmeno of Mycobacteriology and Lung Diseases, Pasteur Institute of Iran, Tehran
3- Department of Microbiology, Isfahan University of Medical Science, Isfahan
Abstract: (15365 Views)
Background and Objectives: Multi locus enzyme electrophoresis (MEE)has been proved to be a powerful
technique in population genetic studies and molecular epidemiology of pathogenic micro-organisms. In this
study MEE was used to determine the genetic relationships of M. kansasii strains cultured from patients at
Pasteur Institute of Iran.
Material and Methods: 21 isolates of M. kansasii (9 isolates from Iran and 12 isolates from other countries) were analyzed for 12 enzymes loci by MEE. Isolate swere grown on LJ slants and BACTEC 13A. The cells were sedimented by centrifugation and their lysates containing the enzymes were extracted by sonication. The horizontal starch gel electrophoresis was used for visualization of enzymes after staining the gels with substrate in solutions or agar overlay.
Results: A considerable genetic diversity was found at different loci of M. kansasii suggesting the existence of different sub-species for this organism. It also showed the inaccuracy of some biochemical test for identification of some isolates with in this species.
Conclusion: Iranian isolates of M. kansasii are genetically diverse. Separation of isolates at high genetic distances in this study suggest the possible existence ofundetected isolates that could fill the gaps between the unrelated isolates.
Material and Methods: 21 isolates of M. kansasii (9 isolates from Iran and 12 isolates from other countries) were analyzed for 12 enzymes loci by MEE. Isolate swere grown on LJ slants and BACTEC 13A. The cells were sedimented by centrifugation and their lysates containing the enzymes were extracted by sonication. The horizontal starch gel electrophoresis was used for visualization of enzymes after staining the gels with substrate in solutions or agar overlay.
Results: A considerable genetic diversity was found at different loci of M. kansasii suggesting the existence of different sub-species for this organism. It also showed the inaccuracy of some biochemical test for identification of some isolates with in this species.
Conclusion: Iranian isolates of M. kansasii are genetically diverse. Separation of isolates at high genetic distances in this study suggest the possible existence ofundetected isolates that could fill the gaps between the unrelated isolates.
Type of Study: Original Research Article |
Subject:
Molecular Epidemiology
Received: 2013/11/14 | Accepted: 2013/11/14 | ePublished: 2013/11/14
Received: 2013/11/14 | Accepted: 2013/11/14 | ePublished: 2013/11/14
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