year 18, Issue 3 (May - June 2024)                   Iran J Med Microbiol 2024, 18(3): 190-199 | Back to browse issues page


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Javdani Shahedin G, Yazdanizad M, Mousavi Nasab S D, Doroud D, Shokri R, Mazaheri Nezhad Fard R. Prevalence of Enteric Viruses in Raw Seafood Collected from the Retail Shops in Tehran, Iran, 2022–2023. Iran J Med Microbiol 2024; 18 (3) :190-199
URL: http://ijmm.ir/article-1-2389-en.html
1- Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran
2- Department of Medical Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3- Department of Arboviruses and Viral Hemorrhagic Fevers, Pasteur Institute of Iran, Tehran, Iran
4- Deputy of Research, Pasteur Institute of Iran, Tehran, Iran
5- Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , raminmazaheri@gmail.com
Abstract:   (843 Views)

Background and Aim: There are still risks of contamination of seafood by the common pathogens such as bacteria and viruses, despite significant achievements in the food security and environmental protection in recent years. The aim of this study was to investigate the prevalence of pathogenic viruses linked to the seafood hygiene in the raw seafood samples collected from the seafood retailers in Tehran, Iran.
Materials and Methods: In general, 200 samples (100 fish and 100 shrimps) were collected from the seafood retailers in Tehran, Iran. For the RNA viruses, the RNA was extracted from the samples and subjected to cDNA synthesis and polymerase chain reaction (PCR) to amplify the target genes. For the adenovirus, DNA genome was extracted and directly used in PCR. Data were analyzed using the SPSS Statistics 29.0.10.
Results: From 100 fish samples, 48, 24, 32, 35, 56 and 55 samples were contaminated with adenovirus, astrovirus, hepatitis A virus, hepatitis E virus, rotavirus and norovirus, respectively. From 100 shrimp samples, 51, 47, 28, 47, 49 and 47 samples were contaminated with adenovirus, astrovirus, hepatitis A virus, hepatitis E virus, rotavirus and norovirus, respectively.
Conclusion: In conclusion, raw seafood can still be a major public health concern. Therefore, it is strongly recommended to use the organized monitoring systems and improved hygiene measurements to decrease the risks of the seafood contaminations and hence foodborne diseases and outbreaks in consumers.

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Type of Study: Original Research Article | Subject: Food Microbiology
Received: 2024/05/1 | Accepted: 2024/07/27 | ePublished: 2024/08/18

References
1. Hajipour N, Valizadeh H, Ketzis J. A review on fish‐borne zoonotic parasites in Iran. Vet Med Sci. 2023;9(2):748-77. [DOI:10.1002/vms3.981] [PMID] [PMCID]
2. Iwamoto M, Ayers T, Mahon BE, Swerdlow DL. Epidemiology of seafood-associated infections in the United States. Clin Microbiol Rev. 2010;23(2):399-411. [DOI:10.1128/CMR.00059-09] [PMID] [PMCID]
3. Afreen M, Bagdatli I. Food-borne pathogens in seafood. Eurasian J Agric Res. 2021;5(1):44-58.
4. Costantini V, Loisy F, Joens L, Le Guyader FS, Saif LJ. Human and animal enteric caliciviruses in oysters from different coastal regions of the United States. Appl Environ Microbiol. 2006;72(3):1800-9. [DOI:10.1128/AEM.72.3.1800-1809.2006] [PMID] [PMCID]
5. Di Cola G, Fantilli AC, Pisano MB, Re VE. Foodborne transmission of hepatitis A and hepatitis E viruses: A literature review. Int J Food Microbiol. 2021;338:108986. [DOI:10.1016/j.ijfoodmicro.2020.108986] [PMID]
6. Bosch A, Pinto R, Lees D, von Bonsdorff C-H, Croci L, De Medici D, et al. 12- Detecting virus contamination in seafood. In Improving Seafood Products for the Consumer. 2008. pp.194-211. Sawston, U.K.: Woodhead Publishing. [DOI:10.1533/9781845694586.3.194] [PMCID]
7. Amroabadi MA, Rahimi E, Shakerian A, Momtaz H. Incidence of hepatitis A and hepatitis E viruses and norovirus and rotavirus in fish and shrimp samples caught from the Persian Gulf. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia. 2021;73:169-78. [DOI:10.1590/1678-4162-11742]
8. Ali A, Parisi A, Conversano M, Iannacci A, D'Emilio F, Mercurio V, et al. Food-borne bacteria associated with seafoods: A brief review. J Food Qual Hazards Control. 2020;4-10. [DOI:10.18502/jfqhc.7.1.2446]
9. Shojaie P, Mashak Z, Koohdar V. Molecular Detection of Norwalk Virus in Carp Fish and Shrimp Ponds in Khuzestan Province, Iran by RT-PCR Method. Int J Enteric Pathog. 2021;9(1):3-8. [DOI:10.34172/ijep.2021.02]
10. Liu S, Zhao K, Huang M, Zeng M, Deng Y, Li S, et al. Research progress on detection techniques for point-of-care testing of foodborne pathogens. Front bioeng biotechnol. 2022;10:958134. [DOI:10.3389/fbioe.2022.958134] [PMID] [PMCID]
11. Liu-Lastres B, Ogbeide G-C. Norovirus on Cruise Ships: Should Americans Be Worried?. J Hosp Tour Cases. 2020;8(2):23-33. [DOI:10.1177/216499872000800204]
12. Yuan K, Huang G, Wang L, Wang T, Liu W, Jiang H, et al. Predicting norovirus in the United States using Google Trends: infodemiology study. J Med Internet Res. 2021;23(9):e24554. [DOI:10.2196/24554] [PMID] [PMCID]
13. Ghalyoun F, Ünver Alçay A. Investigation of Rotavirus, Adenovirus and Astrovirus in Mussels and Shrimps Using Multiplex Real-time PCR. Kafkas Univ Vet Fak Derg. 2018;24(5):761-7. [DOI:10.9775/kvfd.2018.19997]
14. Atmar RL, Neill FH, Romalde JL, Le Guyader F, Woodley CM, Metcalf TG, et al. Detection of Norwalk virus and hepatitis A virus in shellfish tissues with the PCR. Appl Environ Microbiol. 1995;61(8):3014-8. [DOI:10.1128/aem.61.8.3014-3018.1995] [PMID] [PMCID]
15. Papafragkou E, Kulka M. Review: Approaches to the Viral Extraction, Detection, and Identification of Hepatitis Viruses, HAV and HEV, in Foods. J AOAC Int. 2016;99(1):130-42. [DOI:10.5740/jaoacint.15-0164] [PMID]
16. Kotsiri Z, Vidic J, Vantarakis A. Applications of biosensors for bacteria and virus detection in food and water-A systematic review. J Environ Sci. 2022;111:367-79. [DOI:10.1016/j.jes.2021.04.009] [PMID]
17. Ge H, Wang Y, Zhao X. Research on the drug resistance mechanism of foodborne pathogens. Microb Pathog. 2022;162:105306. [https://doi.org/10.1016/j.micpath.2021.105306] [PMID]
18. Kamboj S, Gupta N, Bandral JD, Gandotra G, Anjum N. Food safety and hygiene: A review. Int J Chem Res. 2020;8(2):358-68. [DOI:10.22271/chemi.2020.v8.i2f.8794]
19. Meng J, Doyle M. Emerging and evolving microbial foodborne pathogens. Bulletin de l'Institut Pasteur. 1998;96(3):151-63. [DOI:10.1016/S0020-2452(98)80010-9]
20. Sarno E, Pezzutto D, Rossi M, Liebana E, Rizzi V. A review of significant European foodborne outbreaks in the last decade. J Food Prot. 2021;84(12):2059-70. [DOI:10.4315/JFP-21-096] [PMID]
21. Tarris G, de Rougemont A, Charkaoui M, Michiels C, Martin L, Belliot G. Enteric viruses and inflammatory bowel disease. Viruses. 2021;13(1):104. [DOI:10.3390/v13010104] [PMID] [PMCID]
22. Richards GP. Shellfish-associated enteric virus illness: Virus localization, disease outbreaks and prevention. Viruses Food. 2016:185-207. [DOI:10.1007/978-3-319-30723-7_7] [PMCID]
23. Ahmad J, Ahmad M, Usman AR, Al-Wabel MI. Prevalence of human pathogenic viruses in wastewater: A potential transmission risk as well as an effective tool for early outbreak detection for COVID-19. J Environ Manag. 2021;298:113486. [DOI:10.1016/j.jenvman.2021.113486] [PMID] [PMCID]
24. Suffredini E, Le Q, Di Pasquale S, Pham T, Vicenza T, Losardo M, et al. Occurrence and molecular characterization of enteric viruses in bivalve shellfish marketed in Vietnam. Food Control. 2020;108:106828. [DOI:10.1016/j.foodcont.2019.106828]
25. Lattos A, Chaligiannis I, Papadopoulos D, Giantsis IA, Petridou EI, Vafeas G, et al. How safe to eat are raw bivalves? Host pathogenic and public health concern microbes within mussels, oysters and clams in Greek markets. Foods. 2021;10(11):2793. [DOI:10.3390/foods10112793] [PMID] [PMCID]
26. Winder N, Gohar S, Muthana M. Norovirus: an overview of virology and preventative measures. Viruses. 2022;14(12):2811. [DOI:10.3390/v14122811] [PMID] [PMCID]
27. Battistini R, Masotti C, Maurella C, Costa E, Orlandi M, Feletti M, et al. Study on the Effect of Relaying on Norovirus Reduction from Crassostrea gigas Oysters. Microorganisms. 2022;10(12):2389. [DOI:10.3390/microorganisms10122389] [PMID] [PMCID]
28. Wu C-Y, Chi H, Liu C-C, Huang Y-C, Huang Y-C, Lin H-C, et al. Clinical characteristics and risk factors for children with norovirus gastroenteritis in Taiwan. J Microbiol Immunol Infect. 2021;54(5):909-17. [DOI:10.1016/j.jmii.2020.07.013] [PMID]
29. Yilmaz H, Bostan K, Turan N, Muratoglu K, Yilmaz A, Ozkul AA, et al. Real-time PCR detection of norovirus in mussels collected from the Bosphorus in Istanbul, Turkey. Food Environ Virol. 2010;2:64-8. [DOI:10.1007/s12560-010-9028-3]
30. Matson DO. Norovirus gastroenteritis in US Marines in Iraq. The University of Chicago Press; 2005. pp. 526-7. [DOI:10.1086/427508] [PMID]
31. Riddle MS, Smoak BL, Thornton SA, Bresee JS, Faix DJ, Putnam SD. Epidemic infectious gastrointestinal illness aboard US Navy ships deployed to the Middle East during peacetime operations-2000-2001. BMC Gastroenterol. 2006;6(1):1-7. [DOI:10.1186/1471-230X-6-9] [PMID] [PMCID]
32. Wadl M, Scherer K, Nielsen S, Diedrich S, Ellerbroek L, Frank C, et al. Food-borne norovirus-outbreak at a military base, Germany, 2009. BMC Infect Dis. 2010;10:1-10. [DOI:10.1186/1471-2334-10-30] [PMID] [PMCID]
33. Lowther J, Bosch A, Butot S, Ollivier J, Mäde D, Rutjes S, et al. Validation of EN ISO method 15216-Part 1-Quantification of hepatitis A virus and norovirus in food matrices. Int J Food Microbiol. 2019;288:82-90. [DOI:10.1016/j.ijfoodmicro.2017.11.014] [PMID]
34. Zhong J, Yang Y, Zhang H, Zhang S, Qu X, Chen Q, et al. Risk assessment of norovirus and hepatitis A virus in strawberries imported into China. Food Sci Nutr. 2023;11(12):8009-8026. [DOI:10.1002/fsn3.3721] [PMID] [PMCID]
35. Hyun JH, Yoon JY, Lee SH. A case-control study of acute hepatitis A in South Korea, 2019. Osong Public Health Res Perspect. 2022;13(5):352. [DOI:10.24171/j.phrp.2022.0141] [PMID] [PMCID]
36. Lin S, Zhang Y-J. Advances in hepatitis E virus biology and pathogenesis. Viruses. 2021;13(2):267. [DOI:10.3390/v13020267] [PMID] [PMCID]
37. Treagus S, Wright C, Baker-Austin C, Longdon B, Lowther J. The foodborne transmission of hepatitis E virus to humans. Food Environ Virol. 2021;13:127-45. [DOI:10.1007/s12560-021-09461-5] [PMID] [PMCID]
38. La Rosa G, Proroga Y, De Medici D, Capuano F, Iaconelli M, Della Libera S, et al. First detection of hepatitis E virus in shellfish and in seawater from production areas in Southern Italy. Food Environ Virol. 2018;10:127-31. [DOI:10.1007/s12560-017-9319-z] [PMID]
39. Vos HJ, Knox CM. The recovery and molecular identification of HAdV-D17 in raw sewage and mussel samples collected in the Eastern Cape province of South Africa. Afr J Infect Dis. 2018;33(1):4-7. [DOI:10.4102/sajid.v33i1.27] [DOI:10.1080/23120053.2017.1368617]
40. Benabbes L, Ollivier J, Schaeffer J, Parnaudeau S, Rhaissi H, Nourlil J, et al. Norovirus and other human enteric viruses in Moroccan shellfish. Food Environ Virol. 2013;5:35-40. [DOI:10.1007/s12560-012-9095-8] [PMID]
41. Bergman H, Henschke N, Hungerford D, Pitan F, Ndwandwe D, Cunliffe N, et al. Vaccines for preventing rotavirus diarrhoea: vaccines in use. Cochrane Database Syst Rev. 2021(11). [DOI:10.1002/14651858.CD008521.pub6] [PMID] [PMCID]
42. Marinho A, Rocha D, Kanai Y, Alves C, Costa D, Sousa A, et al. Rotavirus analyses by SYBR Green real-time PCR and microbiological contamination in bivalves cultivated in coastal water of Amazonian Brazil. J Water Health. 2018;16(6):970-9. [DOI:10.2166/wh.2018.130] [PMID]

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