Antibacterial Effect of Synthetized Selenium Nanoparticles and Ampicillin- Selenium Nanoparticles against Clinical Isolates of Methicillin Resistant Staphylococcus aureus

Mehrbakhsh Bandari, Maryam Alsadat; Asadpour, Leila; Pourahmad, Afshin

year 11, Issue 6 (January - February 2018) Iran J Med Microbiol 2018, 11(6): 184-191 | Back to browse issues page

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Mehrbakhsh Bandari M A, Asadpour L, Pourahmad A. Antibacterial Effect of Synthetized Selenium Nanoparticles and Ampicillin- Selenium Nanoparticles against Clinical Isolates of Methicillin Resistant Staphylococcus aureus. Iran J Med Microbiol 2018; 11 (6) :184-191
URL: http://ijmm.ir/article-1-747-en.html

Antibacterial Effect of Synthetized Selenium Nanoparticles and Ampicillin- Selenium Nanoparticles against Clinical Isolates of Methicillin Resistant Staphylococcus aureus

Maryam Alsadat Mehrbakhsh Bandari¹

, Leila Asadpour

², Afshin Pourahmad³

1- Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran
2- Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran , L.asadpour@yahoo.com
3- Department of Chemistry, Rasht Branch, Islamic Azad University, Rasht, Iran

Abstract: (10462 Views)

Background and Aims: Drug resistance, particularly methicillin resistant in Staphylococcus aureus strain is a major worldwide public health concern. The present study aimed to synthetize selenium nanoparticles, investigate its antibacterial effect and its ability to be used as ampicillin nanocarrier.
Materials and Methods: Selenium nanoparticles were synthetized via chemical regeneration of sodium selenite by L-systein amino acid. Loading of ampicillin on the surface of selenium nanoparticles was done by gradual addition of antibiotic to nanoparticle solution and continuous shaking. Then this attachment was investigated by using UV-Vis spectroscopy, XRD and scanning electron microscope. Antibacterial properties of produced selenium nanoparticles and antibiotic loaded selenium nanoparticles against standard strain of S. aureus and 10 methicillin resistant S. aureus strains were tested by disc diffusion method. Minimum inhibitory concentration (MIC) of bacterial growth was determined by broth macrodilution method.
Results: The produced selenium nanoparticles showed antibacterial effect against all strains of S. aureus. Loading of ampicillin on the surface of selenium nanoparticles enhanced the antimicrobial activity of this drug. The mean MIC of selenium nanoparticles against S. aureus strains ranged between 7.8-62.5 µg/mL free form of ampicillin between 62.5- 250 and ampicillin bound to nanoparticles was 7.8-7.8 μg / mL. These values for standard strain of Staphylococcus aureus strains were 7.8, 31.2 and 3.9 μg / mL, respectively. Loading of ampicillin on the surface of selenium nanoparticles enhanced the MIC against methicillin resistant S. aureus.
Conclusions: The obtained results confirmed antibacterial activity of selenium nanoparticles. Enhancing the antibacterial activity of antibiotics loaded on the surface of nanoparticles, increases the possibility of application of these drugs especially for the elimination of hard-to-heal infective diseases.

Keywords: Selenium Nanoparticles (Se NPs), Antibacterial Activity, Methicillin Resistant S. aureus, Ampicillin

Full-Text [PDF 991 kb] (4206 Downloads)

Type of Study: Original Research Article | Subject: Antimicrobial Substances
Received: 2017/08/7 | Accepted: 2018/01/14 | ePublished: 2018/03/19

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