year 11, Issue 4 (September - October 2017)                   Iran J Med Microbiol 2017, 11(4): 77-82 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shariat A. Detection of Epstein-Barr Virus in Hodgkin's lymphoma Specimens in the Fars Province in 2016. Iran J Med Microbiol. 2017; 11 (4) :77-82
Department of Microbiology, Faculty of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran ,
Abstract:   (5996 Views)
Background and Aims: Epstein Barr virus is associated with nasopharyngeal carcinoma, Burkitt lymphoma and Hodgkin's lymphoma. The aim of this study was to detect the frequency of EBV in tissue specimens from patients with Hodgkin's lymphoma in the Fars province using the immunohistochemical method.
Materials and Methods: A number of 30 cases of Hodgkin’s lymphoma tissue samples were selected from formalin-fixed paraffin embedded blocks from the Fars province hospitals in 2016 and the expression of LMP1 was evaluated using the immunohistochemical method. Data were analyzed using SPSS statistical software and Fisher’s exact test.
Results and Conclusions: A total of 77% of the samples (23 of 30), were presented with Epstein-Barr virus and positive cases included 16 males and 7 females. Eighty seven percent (7 of 8) of the Hodgkin’s lymphoma patients infected with EBV were in the age group 1-14 years, 60% (3 of 5) in the age group of 15-49 years and 76% (13 of 17) in the age group over 49 years. The highest expression rate of the virus was seen among mixed cellularity Hodgkin’s lymphoma subtype (93%). According to the Fisher’s exact test, there was no significant correlation in the prevalence of EBV subtypes among different sex and age groups (p values > 0.05). The results showed a high expression of EBV among Hodgkin’s lymphoma specimens of children and adults in the Fars province. Also, the prevalence of EBV was higher among males compared to females and mixed cellularity was the predominant subtype. These results are similar to that in other developing countries.
Full-Text [PDF 514 kb]   (3170 Downloads)    
Type of Study: Brief Original Article | Subject: Medical Virology
Received: 2017/07/17 | Accepted: 2017/09/2 | ePublished: 2017/09/12

1. Montes-Moreno S, Odqvist L, Diaz-Perez JA, Lopez AB, de Villambrosía SG, Mazorra F, et al. EBV-positive diffuse large B-cell lymphoma of the elderly is an aggressive postgerminal center B-cell neoplasm characterized by prominent nuclear factor-kB activation. Mod Pathol 2012;25(7):968–82. [DOI] [PubMed]
2. Azhar M, Din H, Muhammad I, Hashmi SN, Akhtar F. Frequency of Epstein Barr virus in classical Hodgkin lymphoma. J Ayub Med Coll Abbottabad 2016;28(2):271–5. [PubMed]
3. Cickusic E, Mustedanagic-Mujanovic J, Iljazovic E, Karasalihovic Z, Skaljic I. Association of Hodgkin lymphoma with Epstein Barr virus infection. BJBMS 2007; 7(1):58-65.
4. Re D, Kuppers R, Diehl V. Molecular pathogenesis of Hodgkin's lymphoma. J Clin Oncol 2005;23(26):6379-86. [DOI] [PubMed]
5. Küppers R. B cells under influence: transformation of B cells by Epstein-Barr virus. Nat Rev Immunol 2003;3(10):801-12. [DOI] [PubMed]
6. Mozafari L, Najafipour S, Meshkibaf MH, Moravej A. Analysis of the presence of Epstein-Barr virus in Hodgkin's lymphoma in Iranian children by EBER in situ hybridization. Par J Med Sci 2014;12(2):57-63. [DOI]
7. Alebouyeh M, Vossough P. Hodgkin disease in Iranian children. Eur J Pediatr 1993; 152(1):21-3. [DOI] [PubMed]
8. Fu Q, He C, Mao ZR. Epstein-Barr virus interactions with the Bcl 2 protein family and apoptosis in human tumor cells. J Zhejiang Univ Sci B 2013;14(1):8–24. [DOI] [PubMed]
9. Brocqueville G, Ndour PA, Ouk TS, Le Goff A, De Witte C, Mougel A, et al. LMP1- induced cell death may contribute to the emergency of its oncogenic property. PLoS One 2013;8(4):e60743. [DOI] [PubMed]
10. Salamon D, Adori M, Ujvari D, Wu L, Kis LL, Madapura HS, et al. Latency type- dependent modulation of Epstein-Barr virus-encoded latent membrane protein 1 expression by type I interferons in B cells. J Virol 2012;86(8):4701–7. [DOI] [PubMed]
11. Shariat A. Detection of LMP1 protein in Breast Cancer tissue samples from Fars province hospitals. Iran J Med Microbiol 2017;11(1):67-74.
12. Engel M, Essop MF, Close P, Hartley P, Pallesen G, Sinclair-Smith C. Improved prognosis of Epstein-Barr virus associated childhood Hodgkin’s lymphoma: study of 47 South African cases. J Clin Pathol 2000;53(3):182–6. [DOI] [PubMed]
13. Mohamed G, Vrzalikova K, ZumlaCader F, Vockerodt M, Nagy E, Flodr P, et al. Epstein–Barr virus, the germinal centre and the development of Hodgkin’s lymphoma. J Gen Virol 2014;95(9):1861–9. [DOI] [PubMed]
14. Jarrett AF, Armstrong AA, Alexander E. Epidemiology of EBV and Hodgkin's lymphoma. Ann Oncol 1996;7(4):S5-S10. [DOI]
15. Hjalgrim H, Engels EA. Infectious aetiology of Hodgkin and non-Hodgkin lymphomas: a review of the epidemiological evidence. J Intern Med. 2008;264(6):537-48. [DOI] [PubMed]
16. Katebi M, Sharifi N, Tarhini M, Otrock ZK, Bazarbachi A, Kchour G. Frequency of Epstein-Barr virus expression in various histological subtypes of Hodgkin's lymphoma. Histopathology 2008;52(6):775-7. [DOI] [PubMed]
17. Najafipour S, Mokhtari Azad T, Kousari F, Mahmoodi M, Nategh R. Association of Epstein-Barr Virus and Hodgkin’s Disease. Acta Medica Iranica 2003;41(1):1-10.
18. Mozafari L, Najafipour S, Meshkibaf MH, Moravej A. Expression of Epstein-Barr virus in Hodgkin lymphoma Specimens in IRAN. JFUMS 2013;3(2):143-8.
19. Jadali F, Karimi A, Fallah F, Habibi G, Shamshiri A, Gharib A, et al. Detection of Epstein-Barr virus in Pediatric Hodgkin's Lymphoma in Iran by immunohistochemistry and in situ hybridization. Cancer Ther 2008;6(2):665-71.
20. Grywalskaand E, Rolinski J. Epstein-Barr Virus–Associated Lymphomas. Semin Oncol 2015;42(2):291-303. [DOI] [PubMed]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc