Rezaei M, Edalat R, Sohrabi A, Siadat S D, Motamedirad M, Vand Yousefi J et al . The Survey of prevalence of acute gastroenteritis caused by entric Adenoviruse type Ad40, Ad41 in hospitalized children younger than 5 years in Bahrami hospital in Tehran by PCR method,2008-2009. Iran J Med Microbiol 2010; 4 (1 and 2) :74-79
URL:
http://ijmm.ir/article-1-62-en.html
1- Department of Microbiology- Azad Islamic University of Karaj, Iran
2- Department of Virology, Pasteur Institute of Iran
3- Department of Hepatitis and AIDS , Pasteur Institute of Iran
4- Department of Virology, Pasteur Institute of Iran , Mary_1486@yahoo.com
Abstract: (15631 Views)
Background and Objectives: Human Adenoviruses are one of the most common and serious
etiologic agents of acute gastroenteritis in children younger of 5 years old in word wide,
especially developing countries. The purpose study was a survey of molecular epidemiology of
enteric Adenoviruses in acute gastroenteritis in hospitalized children in Bahrami hospital in
Tehran, 2008-2008.
Materials and Methods: We obtained 100 stool specimens from hospitalized children under 5
years of age with clinical presentation of acute gastroenteritis in Tehran from June 2008 to June
2009. Viral DNA was extracted by extraction kit of DNA. Then extracted viral DNA by use of
PCR method with genus specific and type 40, 41 primers, enteric Adenoviruses were
diagnosed.
Results: Adenoviruses Ad40, Ad41 have been detected in 8 (8%) of patients with diarrhea. The
highest prevalence of infection occurred in children aged 0 to 24 months (75%). The rates of
infection were 60% for male and 40%for females. Our data indicated that the outbreaks of
Adenoviruses infection were in winter(50%).The frequency of the Adenoviruses Ad40,Ad41
among breast-fed and bottle-fed, was 12.5%and 62.5%respectively.
Conclusion: Molecular diagnostic methods should be with high features such as PCR and
effective step in the recognition of these factors can be harvested as the appropriate strategy for
treatment adopted.
Type of Study:
Original Research Article |
Subject:
Nosocomial infections Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10