Evaluation of the sensitivity of the PCR technique in detecting genes omp31, omp25 and bcsp31 in patients with brucellosis

Alizadeh Mofrad, Farzad; Alizadeh Mofrad, Sara; Dolatshahi, Farid; Kolahi, Mustafa; saber Mohammadi, Saber

year 10, Issue 4 (September - October 2016) Iran J Med Microbiol 2016, 10(4): 63-68 | Back to browse issues page

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Alizadeh Mofrad F, Alizadeh Mofrad S, Dolatshahi F, Kolahi M, saber Mohammadi S. Evaluation of the sensitivity of the PCR technique in detecting genes omp31, omp25 and bcsp31 in patients with brucellosis. Iran J Med Microbiol 2016; 10 (4) :63-68
URL: http://ijmm.ir/article-1-548-en.html

Evaluation of the sensitivity of the PCR technique in detecting genes omp31, omp25 and bcsp31 in patients with brucellosis

Farzad Alizadeh Mofrad

¹, Sara Alizadeh Mofrad²

, Farid Dolatshahi³

, Mustafa Kolahi⁴

, Saber Saber Mohammadi⁵

1- Department of Biology, Faculty of Science, Islamic Azad University of Urmia, Urmia,Iran , KHASHAYARSHA2500@GMAIL.COM
2- Department of Biology, Faculty of Science, Islamic Azad University of damghan, damghan,Iran
3- Department of Biology, Faculty of Science, Islamic Azad University of Kkorramabad, Kkorramabad,Iran
4- Department of Biology, Faculty of Science, Islamic Azad University of saveh, saveh,Iran
5- Department of Biology, Faculty of Science, Islamic Azad University of Urmia, Urmia,Iran

Abstract: (8782 Views)

Background and Aim: Brucellosis is still one of the common infections in developing countries and some developed countries. Diagnosis of the human brucellosis is mainly based on blood culture and serological tests.also in addition to determine the root cause of brucellosis, the aim of this paper is susceptibility assessment of three bcsp31, omp31 and omp25 in the diagnosis of brucellosis using PCR method among people with brucellosis.

Materials and Methods: In this study, All the patients were diagnosed with positive immunological tests such as Wright and ELISA. samples of blood were cultured (BACTEC) and incubated at 37°C for 5 days and then they were cultured for 3 days on Brucella agar. DNA was extracted from the colonies by Kit. The extracted DNA was used as template for accurate diagnosis of bacterial strains with gene-specific primers in PCR reactions bcsp31, omp31 and omp25.

Results and Conclusions: After the PCR reaction, results show that there is 100% PCR power for diagnosis of bscp31, 90.47% sensitivity for omp31 and 85.7% sensitivity for omp25 genes. Due to the high power and specificity and existence of bcsp31 in human pathogenic species, bcsp31 gene can be considered as more appropriate and confident target gene than omp31 and omp25 genes in the diagnosis of brucellosis in clinical samples using PCR method.

Keywords: Brucellosis, PCR, Detection Power, omp25, omp31, bcsp31

Full-Text [PDF 504 kb] (2578 Downloads)

Type of Study: Brief Original Article | Subject: Medical Bacteriology
Received: 2016/04/13 | Accepted: 2016/04/17 | ePublished: 2016/10/17

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