year 10, Issue 3 (July - August 2016)                   Iran J Med Microbiol 2016, 10(3): 47-53 | Back to browse issues page

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Bineshian F, Irajian G, Bagheri Mansoori M H, sharifi Z. The relationship between IL-10 (-819 C/T) polymorphism and Hepatitis B infection. Iran J Med Microbiol. 2016; 10 (3) :47-53
1- Department of Mycology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
2- Department of Microbiology, Iran University of Medical Science, Tehran, Iran
3- Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
4- Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran ,
Abstract:   (7533 Views)

Background and Aim: Anti-inflammatory cytokine IL-10 is important and single nucleotide polymorphisms in the interleukin-10 gene promoter is associated with HBV infection. Especially single nucleotide polymorphisms in the promoter region of the gene IL10, at position -1082, -819, -592 is associated with production of IL10. Therefore, the present study was to determine the effect of IL-10 -819 polymorphism with chronic HBV infection in Iranian patients.

Materials and Methods: A total of 200 subjects (100 chronically infected hepatitis B patients and 100 blood donors) with an age range of 18-59 years were included in this study randomly.The study in 1392 on samples of patients referred to clinical laboratory blood transfusion was performed. Genomic DNA was extracted from Buffy coat layer with using of salting out procedure.Allele Specific Polymerase Chain Reaction Method was used for determining polymorphisms -819 (C/T) in the IL-10 gene promoter.Electrophoresis was performed on a arose gel 1.5 per cent for the detection of PCR products. Data were analyzed using Chi Square analysis

Results: The frequency of Homozygote genotype IL-10 -819 (TT, TC, CC) was in patients with HBV 84%,12% and 4% and 89%,10% and 1% in healthy individuals respectively  and no significant difference was observed between the two groups in terms of genotype (P =0.8). Variant of C allele frequency was in patients with HBV 10% and 6% in healthy individuals. There was no statistically significant difference between the two groups (P=.0.6).

Conclusions: Our results showed that there was no correlation between polymorphism IL-10-819(T/C) in control group and chronic Hepatitis B infection.

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Type of Study: Original Research Article | Subject: Medical Virology
Received: 2015/09/6 | Accepted: 2016/02/15 | ePublished: 2016/10/16

1. Alam M M, Zaidi S Z. Common Genotypes of Hepatitis B virus prevalent in injecting drug abusers (addicts) of North West Frotier Province of Pakistan. Virol J 2007; 4: 63. [PubMed]
2. Dienstag JL. Hepatitis B virus infection. N Engl J Med 2008; 359(14):1486–1500.
3. Sonneveld MJ, Rijckborst V, Boucher CA, Hansen BE, Janssen HL .Prediction of sustained response to peg interferon alfa-2b for hepatitis B e antigen positive chronic hepatitis B using on treatment hepatitis B surface antigen decline. Hepatology 2010; 52(4):1251–1257. [PubMed]
4. Li GY, Huang M, Pan TT, Jia WD.Expression and prognostic significance of contactin 1 in human hepatocellular carcinoma.Onco Targets Ther 2016; 9:387-94. [PubMed]
5. Lavanchy D .Worldwide epidemiology of HBV infection, disease burden, and vaccine prevention. J Clin Virol 2005; 34(Suppl 1):S1–S3. [PubMed]
6. Yari A, Yousefzadeh H, Tahaghoghi S, Ghazvini K.Prevalence of Hepatitis B and C among Patients Looking for Hospital Care; Five Years' Study in Mashhad, Iran. Acta Med Iran. 2016; 54(1):54-7. [PubMed]
7. Ott JJ, Stevens GA, Groeger J, Wiersma ST. Global epidemiology of hepatitis B virus infection: new estimates of age-specific HBsAg seroprevalence and endemicity. Vaccine. 2012; 30(12):2212-9. [PubMed]
8. Bommireddy R, Doetschman T. Review: TGF-β1 and Treg cells: alliance for tolerance Mol Med 2007; 13(11):492-501. [PubMed]
9. Zhang TC, Zhang WF, Zhao YQ, Pan FM, Gao YF, Yuan variation in IL10 and susceptibility to chronic hepatitis B. J Infect 2014; 69:75–80 [PubMed]
10. Collado-Hidalgo A, Bower JE, Ganz PA,Irwin MR,Cole SW, Cytokine gene polymorphisms and fatigue In Breast cancer survivors : Early findings .Brain Behav Immun 2008;8:8. [PubMed]
11. Thursz M, Yee L, Khakoo S .Understanding the host genetics of chronic hepatitis B and C. Seminars Liver Dis 2011; 31:115–127. [PubMed]
12. Tuncbilek S .Relationship between cytokine gene polymorphisms and chronic hepatitis B virus infection. WJG 2014 ; 20:6226–6235. [PubMed]
13. Shin HD, Park BL, Kim LH, Jung JH, Kim JY, Yoon JH. Interleukin 10 haplotype associated with increased risk of hepatocellular carcinoma. Hum Mol Genet 2003; 12(8): 901-6. [PubMed]
14. Miyazoe S, Hamasaki K, Nakata K, Kajiya Y, Kitajima K, Nakao K, et al. Influence of interleukin-10 gene promoter polymorphisms on disease progression in patients chronically infected with hepatitis B virus. Am J Gastroenterol 2002; 97(8): 2086-92. [PubMed]
15. Wang S, Huang D, Sun S, Ma W, Zhen Q. Interleukin-10 promoter polymorphism predicts initial response of chronic hepatitis B to interferon alfa. Virol J. 2011; 8:28. [PubMed]
16. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988; 16(3): 1215. [PubMed]
17. L Bohiltea C, E Radoi V.Interleukin-6 and interleukin-10 gene polymorphisms and recurrent pregnancy loss in Romanian population. Iran J Reprod Med. 2014; 12(9):617-22.
18. Alavian, S.-M. Ministry of Health in Iran is serious about controlling hepatitis B. Hepat Mon 2007; 7(1): 3-5. [Article]
19. Fuse S, Molloy M J. Immune responses against persistent viral infections: possible avenues for immunotherapeutic interventions. Crit Rev Immunol 2008; 28(2):159-83. [PubMed]
20. Yang G, Liu J, Han S, Xie H, Du R, Yan Y, Association between hepatitis B virus infection and HLA-DRB1 genotyping in Shaanxi Han patients in northwestern China. Tissue Antigens 2007; 69(2): 170-5. [PubMed]
21. Lu YL, Wu X, Huang HL, Dai LC. Allele polymorphisms of interleukin-10 and hepatitis B, C virus infection. Chin Med J 2010; 123:1338-1344. [PubMed]
22. Mohebbatikaljahi H, Menevse S, Yetkin I, Demirci H. Study of interleukin-10 promoter region polymorphisms (−1082A/G, −819T/C and −592A/C) in type 1 diabetes mellitus in Turkish population. Genet 2009; 88(2):245-8.
23. Xue H, Lin B, An J, Zhu Y, Huang G. Interleukin-10-819 promoter polymorphism in association with gastric cancer risk.BMC Cancer 2012; 12:102. [Article]
24. Azarpira N, Borhani Haghighi A, Pourjafar M, Shariat A. Interleukin 10 Gene Polymorphism in Iranian Patients with Multiple Sclerosis . Acta Neurol Taiwan. 2010; 19(2):107-11. [PubMed]
25. Cheong JY, Cho SW, Hwang IL, Yoon SK, LeeJH, Park CS, et al. Association between chronic hepatitis B virus infection and interleukin-10, tumor necrosis factor-alpha gene promoter polymorphisms. J Gastroenterol Hepatol 2006; 21(7): 1163-9. [PubMed]
26. Gao QJ, Liu DW, Zhang SY, Jia M, Wang LM, Wu LH, et al. Polymorphisms of some cytokines and chronic hepatitis B and C virus infection. World J Gastroenterol 2009; 15(44): 5610-9. [PubMed]
27. Wu JF, Ni YH, Lin YT, Lee TJ, Hsu SH, Chen HL, et al. Human interleukin- 10 genotypes are associated with different precore/core gene mutation patterns in children with chronic hepatitis B virus infection. J Pediatr. 2011; 158(5):808-13. [PubMed]
28. Truelove AL, Oleksyk TK, Shrestha S, Thio CL, Goedert JJ, Donfield SM, et al. Evaluation of IL10, IL19 and IL20 gene polymorphisms and chronic hepatitis B infection outcome. Int J Immunogenet 2008; 35(3):255-64. [PubMed]
29. Yan Z, Tan W, Zhao W, Dan Y, Wang X, Mao Q, et al. Regulatory polymorphisms in the IL-10 gene promoter and HBV-related acute liver failure in the Chinese population. J Viral Hepat 2009; 16(11): 775-83. [PubMed]
30. Turner DM, Williams DM, Sankaran D, Lazarus M, Sinnott PJ, Hutchinson IV. An investigation of polymorphism in the interleukin-10 gene promoter. Eur J Immunogenet 1997; 24(1): 1-8. [PubMed]
31. Ren H, Zhang TT, Hu WL.A -819 C/T polymorphism in the interleukin-10 promoter is associated with persistent HBV infection, but -1082 A/G and -592A/C polymorphisms are not: a meta-analysis. Arch Virol 2015; 160(3):747-56.
32. Sofian M, Kalantar E, Aghakhani A, Hosseini S, Banifazl M, Eslamifar A, Jourabchi A, Farazi AA, Ramezani A. No correlation between interleukin-10 gene promoter polymorphisms and hepatitis B virus infection outcome. Hepat Mon. 2013; 13(5):e8803. [PubMed]
33. Talaat RM, Dondeti MF, El-Shenawy SZ, Khamiss OA.Association between IL-10 gene promoter polymorphism and hepatitis B viral infection in an Egyptian population. Biochem Genet. 2014; 52(9-10):387-402. [PubMed]

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