Isolation and Identification of Streptococcus pneumoniae from Admitted Patients in Tehran Hospitals in during 2013-2015

Mohsenpoor, Mohammad; Mehrabi Tavana, Ali; Ataee, Ramezanali; Ranjbar, Reza; Mirnejad, Reza

year 11, Issue 2 (May - June 2017) Iran J Med Microbiol 2017, 11(2): 9-16 | Back to browse issues page

Mendeley

Zotero

RefWorks

Mohsenpoor M, Mehrabi Tavana A, Ataee R, Ranjbar R, Mirnejad R. Isolation and Identification of Streptococcus pneumoniae from Admitted Patients in Tehran Hospitals in during 2013-2015. Iran J Med Microbiol 2017; 11 (2) :9-16
URL: http://ijmm.ir/article-1-406-en.html

Isolation and Identification of Streptococcus pneumoniae from Admitted Patients in Tehran Hospitals in during 2013-2015

Mohammad Mohsenpoor¹

, Ali Mehrabi Tavana

², Ramezanali Ataee³

, Reza Ranjbar⁴

, Reza Mirnejad⁴

1- Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
2- Research Centre for Health Management, Baqiyatallah University of Medical Sciences, Tehran, Iran , mehrab@bmsu.ac.ir
3- Health and Nutrition Research, Baqiyatallah University of Medical Sciences, Tehran, Iran
4- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Abstract: (10196 Views)

Background and Aim: Streptococcus pneumoniae is Gram-positive bacterium that causes pneumonia, septicemia, meningitis, otitis media, sinusitis, bronchitis, bacteremia and infectious processes is introduced. Due to the clinical importance of this bacterium and correct and timely diagnosis and separation of isolated samples from patients, this study were performed to identify and isolate strains of Pneumococcus from clinical samples by culture and PCR methods.

Materials and Methods: In this study, 175 various clinical samples were collected from admitted patients in Tehran hospitals during 3 years (2013-2015),and culture and PCR methods used to isolate and identify of strains of Pneumococcus (after genome extraction and in the presence of specific primer) beside the standard strain ATCC 49619 (as control).

Results: In this study, 40 strains of Pneumococcus have been identified and approved by culture and PCR methods. Amplified amplicon in this study, was a 160 bp fragment that were found in all strains of Streptococcus pneumoniae. Also, the PCR product was confirmed by sequencing.

Conclusions: According to the obtained results in this study, PCR similar to culture, is a reliable method, but has been assessed relatively faster that can be used in the future to replace the old and conventional methods (biochemical tests and culture).

Keywords: Streptococcus pneumoniae, Culture, Genome, Primer, PCR

Full-Text [PDF 751 kb] (5472 Downloads)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2015/02/8 | Accepted: 2016/10/4 | ePublished: 2016/10/16

References

1. Brooks GF, Carroll KC, Butel JS, Morse SA, Mietzner TA. The Streptococci. In: Jawetz, Melnick & Adelberg’s. Medical Microbiology. 25th ed. New York: McGraw-Hill; 2010. p. 219-236. [Article]

2. Driver C. Pneumonia part 2: signs, symptoms and vaccinations. Br J Nurs 2012; 21(4):245-9. [PubMed]

3. Watson DA, Musher DM, Jacobson JW, Verhoef J. A brief history of the Pneumococcus in biomedical research: a panoply of scientific discovery. Clin Infect Dis 1993; 17(5):913-24. [PubMed]

4. O'Brien KL, Wolfson LJ, Watt JP, Henkle E, Deloria-Knoll M, McCall N, et al. Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years: global estimates. Lancet Infect Dis 2009; 374(9693):893-902. [Article]

5. Lawn JE, Wilczynska-Ketende K, Cousens SN. Estimating the causes of 4 million neonatal deaths in the year 2000. Int J Epidemiol 2006; 35(3):706-18. [PubMed]

6. Scott JAG. The global epidemiology of childhood pneumonia 20 years on. Bull World Health Organ 2008; 6(6):494–6. [PubMed]

7. Williams BG, Gouws E, Boschi-Pinto C, Bryce J, Dye C. Estimates of world-wide distribution of child deaths from acute respiratory infections. Lancet Infect Dis 2002; 2(1):25-32. [PubMed]

8. Black RE, Morris SS, Bryce J. Where and why are 10 million children dying every year? Lancet Infect Dis 2003; 361(9376):2226-34. [PubMed]

9. Obaro S, Adegbola R. The Pneumococcus: carriage, disease and conjugate vaccines. J Med Microbiol 2002;51(2):98-104. [PubMed]

10. Bogaert D, De Groot R, Hermans PWM. Streptococcus pneumoniae colonization: the key to Pneumococcal disease. Lancet Infect Dis 2004; 4(3):144-54. [PubMed]

11. Centers for Disease Control and Prevention (CDC). Identification and Characterization of Streptococcus pneumoniae. In: Laboratory Methods for the Diagnosis of Meningitis Caused by Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. 2th ed. Switzerland: WHO; 2011. p.73-86. [Article]

12. Betty A, Daniel F, Alice S. Bailey and Scott’s Diagnostic Microbiology. 11th ed. Saint Louis: Mosby; 2002.

13. Pai R, Gertz RE, Beall B. Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates. J Clin Microbiol 2006; 44(1):124-31. [PubMed]

14. Da Gloria Carvalho M, Pimenta FC, Jackson D, Roundtree A, Ahmad Y, Millar EV, et al. Revisiting Pneumococcal carriage by use of broth enrichment and PCR techniques for enhanced detection of carriage and serotypes. J Clin Microbiol 2010; 48(5):1611-8. [PubMed]

15. Soltan-Dallal MM, Jabbari H, Forushani AR, Heidarzadeh S, Afrogh, Sharifi Yazdi MK. Frequency and resistance patterns of Streptococcus pneumoniae in acute otitis media. J Mazand Univ Med Sci 2013; 23(98):28-35. [in Persian] [Article]

16. Ataee RA, Habibian S, Mehrabi-Tavana A, Ahmadi Z, Jonaidi N, Salesi M. Determination of vancomycin minimum inhibitory concentration for ceftazidime resistant Streptococcus pneumoniae in Iran. Ann Clin Microbiol Antimicrob 2013; 13(1):53-59. [PubMed]

17. Ahmadi A, Talebi M, Sayahfar S, Irajian G. Accuracy of detection of Streptococcus pneumoniae in clinical laboratories by using phenotypic and molecular methods. Koomesh 2015; 16(3):384-8. [in Persian] [Article]

18. Aghamiri F, Soleimani M, Mohseni AH, Majidzadeh K. Design of an improved polymerase chain reaction (PCR) assay for molecular detection of Streptococcus pneumonia. Qom Univ Med Sci J 2013; 7(4):81-88. [in Persian] [Article]

19. Irajian G, Ahmadi A, Talebi M. The study of Streptococcus pneumoniae in invasive and non invasive infections and multiplex PCR detection of four virulence genes. IEM J 2013; 1(1):3-8. [in Persian] [Article]

20. Hajia M, Rahbar M, Rahnami Farzami M, Dolatyar A, Imani M, Saburian R, et al. Efficacy of multiplex PCR procedure for Iranian Streptococcus pneumoniae isolates. Caspian J Intern Med 2014; 5(2):109-13. [PubMed]

21. Ghotaslou R, Farajnia S, Yeganeh F, Abdoli-Oskouei S, Ahangarzadeh-Rezaee M, Barzegar M. Detection of acute childhood meningitis by PCR, culture and agglutination tests in Tabriz, Iran. Acta Med Iran 2012; 50(3):192-6. [PubMed]

22. Nomanpour B, Ghodousi A, Babaei T, Mousavi SA, Asadi S, Feizabadi M. Detection and quantification of Streptococcus pneumoniae from Iranian patients with pneumonia and ndividual carriers by Real-Time PCR. AJB 2011; 10(60):12826-32. [Article]