year 19, Issue 5 (September - October 2025)
Iran J Med Microbiol 2025, 19(5): 354-368 |
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Alam S, Farooq U. Detection of mecA, vanA and vanB Genes Among Methicillin- Resistant Staphylococcus aureus Isolates from Various Clinical Specimens in Moradabad, India. Iran J Med Microbiol 2025; 19 (5) :354-368
URL: http://ijmm.ir/article-1-2792-en.html
URL: http://ijmm.ir/article-1-2792-en.html
1- Department of Microbiology, Teerthanker Mahaveer University, Moradabad, India , shanealam5361@gmail.com
2- Department of Microbiology, Teerthanker Mahaveer University, Moradabad, India
2- Department of Microbiology, Teerthanker Mahaveer University, Moradabad, India
Abstract: (253 Views)
Background and Aim: The most effective antibiotic for treating methicillin-resistant Staphylococcus aureus (MRSA) infections is vancomycin. However, vancomycin-intermediate Staphylococcus (S.) aureus (VISA) and vancomycin-resistant S. aureus (VRSA) have been found to be more common in various parts of the world. This study aimed to determine the prevalence of mecA, vanA and vanB genes among MRSA isolates and to categorize vancomycin susceptibility profiles in clinical specimens from TMMC & RC, Moradabad.
Materials and Methods: In the central laboratory of TMMC and RC Moradabad, S. aureus was isolated from various clinical samples and MRSA was detected by Kirby-Bauer disk diffusion method. Minimum inhibitory concentration (MIC) of vancomycin was determined by broth microdilution (BMD) method and categorized it into VSSA, VISA, and VRSA isolates. The mecA, vanA and vanB genes were detected on extracted genomic DNA using polymerase chain reaction.
Results: Out of 314 MRSA strains, 296 (94.27%) were VSSA, 14 (4.46%) were VISA and 4 (1.27%) were VRSA, as found on the basis of MIC determination through BMD method. All MRSA strains possessed the mecA gene, 1 VRSA had vanA and 3 VRSA had vanB. Among VISA isolates no vanA and vanB genes were detected.
Conclusion: The high prevalence of MRSA (52.5%) and the emergence of VRSA (1.27%) carrying vanA and vanB genes in our setting highlight a serious public health threat and underscore the need for continuous surveillance and stringent infection control measures.
Keywords: Cefoxitin, Methicillin-resistant Staphylococcus aureus, Staphylococcus aureus, Vancomycin, Vancomycin-intermediate Staphylococcus aureus, Vancomycin-resistant Staphylococcus aureus
Type of Study: Original Research Article |
Subject:
Medical Bacteriology
Received: 2025/07/27 | Accepted: 2025/10/28 | ePublished: 2025/11/11
Received: 2025/07/27 | Accepted: 2025/10/28 | ePublished: 2025/11/11
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