year 17, Issue 4 (July - August 2023)                   Iran J Med Microbiol 2023, 17(4): 457-463 | Back to browse issues page


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Majid Jameel Y, Kassem Khalil Z. Impact of Papillomavirus Infections on Immunoglobulin Levels and Cytokine Correlations in Iraqi Women: A Study from January to August 2022. Iran J Med Microbiol 2023; 17 (4) :457-463
URL: http://ijmm.ir/article-1-2200-en.html
1- Medical Technical Institute Al-Mansour, Middle Technical University (MTU), Baghdad, Iraq
2- Medical Technical Institute Al-Mansour, Middle Technical University (MTU), Baghdad, Iraq , zenakassem2009@gmail.com
Abstract:   (822 Views)

Background and Aim: Human papillomavirus (HPV) is a widespread sexually transmitted infection (STI) with significant health implications. This study aimed to assess the reactivity of TGF, IL10, and MCP-1 with Leukotriene B4 in women diagnosed with cervical HPV infections. Additionally, we considered the histological grade and clinical stage of the patients.
Materials and Methods: In this observational study, a total of 50 cervical infection samples from women diagnosed with HPV and 50 samples from healthy controls were collected between January 2nd and August 1st, 2022, from attendees of Al-Elwea Hospital for Delivery and Children. IgM and IgG antibodies against HPV antigens were detected in patient sera. Polymerase chain reaction (PCR) was employed to amplify the IL-6 gene sequences of lengths 249 and 431 base pairs. Interleukin levels were quantified using the enzyme-linked immunosorbent assay (ELISA) technique. HPV infection diagnosis was confirmed using cervical cytology (Pap smear) and HPV DNA testing before study inclusion.
Results: The average age of the patients was [35.02±10.73]. The findings revealed that among papilloma cases, 30 (85.7%) exhibited normal levels of both IgM and IgG, while 5 cases (14.3%) showed normal IgM levels and elevated IgG levels. Additionally, 10 cases (66.7%) of papilloma demonstrated increased IgM levels and normal IgG levels, in contrast to 5 cases (33.3%) with elevated levels of both IgM and IgG. Importantly, a positive correlation was observed between IgM and IgG levels and the concentrations of TGF, MCP-1, IL-10, and LTB-4 (r=0.896**, 0.678**, 0.692**, 0.894**), (r=0.894**, 0.546**, 0.570**, 0.618**), respectively.
Conclusion: The study revealed a significant positive correlation between IgM and IgG levels and the concentrations of TGF, MCP-1, IL-10, and LTB-4, indicating potential immunological interactions in HPV infection. High Ct values for both patients and controls, along with HR-HPV gene expression, suggest a proportional relationship between gene concentration and Ct value.

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Type of Study: Original Research Article | Subject: Medical Virology
Received: 2023/06/17 | Accepted: 2023/09/9 | ePublished: 2023/09/27

References
1. Verrier F, Le Coeur S, Delory T. Cervical human papillomavirus infection (HPV) and high oncogenic risk genotypes among women living with HIV in Asia: A meta-analysis. J Clin Med. 2021;10(9):1911. [DOI:10.3390/jcm10091911] [PMID] [PMCID]
2. Evans M, Lawson A, Jarin JD, Wilson EE. Squamous Carcinoma of the Cervix in a 15-Year-Old with Congenital HIV: A Case Report. J Pediatr Adolesc Gynecol. 2021;34(2):234-7. [DOI:10.1016/j.jpag.2020.08.014] [PMID]
3. Ghoreshi ZA, Molaei HR, Arefinia N. The Role of DNA Viruses in Human Cancer. Cancer Inform. 2023;22:11769351231154186. [DOI:10.1177/11769351231154186] [PMID] [PMCID]
4. Desai S, Zhu MJ, Lapidos‐Salaiz I. Cervical cancer prevention: Human papillomavirus testing as primary screening. Cancer. 2022;128(5):939-43. [DOI:10.1002/cncr.34006] [PMID]
5. Kuguyo O, Dube Mandishora RS, Thomford NE, Makunike-Mutasa R, Nhachi CF, Matimba A, et al. High-risk HPV genotypes in Zimbabwean women with cervical cancer: Comparative analyses between HIV-negative and HIV-positive women. PLoS One. 2021;16(9):e0257324. [DOI:10.1371/journal.pone.0257324] [PMID] [PMCID]
6. Adebamowo SN, Adeyemo AA, Rotimi CN, Olaniyan O, Offiong R, Adebamowo CA. Genome-wide association study of prevalent and persistent cervical high-risk human papillomavirus (HPV) infection. BMC Med Genet. 2020;21(1):1-10. [DOI:10.1186/s12881-020-01156-1] [PMID] [PMCID]
7. Ntanasis-Stathopoulos I, Kyriazoglou A, Liontos M, Dimopoulos M, Gavriatopoulou M. Current trends in the management and prevention of human papillomavirus (HPV) infection. J BUON. 2020;25(3):1281-5. [PMID]
8. Patel A, Patel R. Recent insights into HSV infection and disease: results of wider genome analysis. Curr. Opin Infect Dis. 2019;32(1):51-5. [DOI:10.1097/QCO.0000000000000512] [PMID]
9. Alizon S, Murall CL, Bravo IG. Why human papillomavirus acute infections matter. Viruses. 2017;9(10):293. [DOI:10.3390/v9100293] [PMID] [PMCID]
10. Gravitt PE, Winer RL. Natural history of HPV infection across the lifespan: role of viral latency. Viruses. 2017;9(10):267. [DOI:10.3390/v9100267] [PMID] [PMCID]
11. Walker FC, Sridhar PR, Baldridge MT. Differential roles of interferons in innate responses to mucosal viral infections. Trends Immunol. 2021; 42(11):1009-23. [DOI:10.1016/j.it.2021.09.003] [PMID] [PMCID]
12. Chauhan P, Nair A, Patidar A, Dandapat J, Sarkar A, Saha B. A primer on cytokines. Cytokine. 2021; 145:155458. [DOI:10.1016/j.cyto.2021.155458] [PMID]
13. Baumjohann D, Fazilleau N. Antigen‐dependent multistep differentiation of T follicular helper cells and its role in SARS‐CoV‐2 infection and vaccination. Eur J Immunol. 2021;51(6):1325-33. [DOI:10.1002/eji.202049148] [PMID] [PMCID]
14. Rasiuk AS, Walsh SR, Chan L, Viloria-Petit AM, Wootton SK, Karimi K, et al. The role of type I interferon signaling in regulating cytokine production and cell survival in bone marrow-derived macrophages. Viral Immunol. 2021;34(7):470-82. [DOI:10.1089/vim.2020.0308] [PMID]
15. She YX, Yu QY, Tang XX. Role of interleukins in the pathogenesis of pulmonary fibrosis. Cell Death Discov. 2021;7(1):52. [DOI:10.1038/s41420-021-00437-9] [PMID] [PMCID]
16. Al-Sarray AJA, Al-Kayat T, Mohammed BM, Al-assadi MJB, Abu-Zaid Y. Dielectric and Electrical Properties of Intercalated 1-(4-nitrophenyl)-N-(p-tolyl) methanimine into the Interlayers of Bentonite Clay. J Med Chem Sci. 2022;5(7(Special Issue)):1321-30.
17. Dhumad Q. Expression of HPV-16 L1 gene human papillomavirus (HTLV-1) identified by pap smear. Arch Razi Inst. 2022;77(6):2125.
18. Majid Jameel Y, Kassem Khalil Z. Relation between Serological Findings and Expression of IL-1β and IL-6 genes in Patients Infected with Mycobacterium Tuberculosis in Iraq. Arch Razi Inst. 2022;77(4):1497-502.
19. Kim KY, Kim DY, Seo J, Ahn Y, Kim DS. Increased serum antibody titer against HPV-16 antigen in patients with Behçet's disease. J Korean Med Sci. 2017;32(4):599-604. [DOI:10.3346/jkms.2017.32.4.599] [PMID] [PMCID]
20. Jamshidi M, Shekari M, Nejatizadeh AA, Malekzadeh K, Baghershiroodi M, Davudian P, et al. The impact of human papillomavirus (HPV) types 6, 11 in women with genital warts. Arch Gynecol Obstet. 2012;286:1261-7. [DOI:10.1007/s00404-012-2416-1] [PMID]
21. Zafari E, Soleimanjahi H, Samiee S, Razavinikoo H, Farahmand Z. Comparison of methylation patterns of E6 gene promoter region in the low-risk and high-risk human papillomavirus. Iran J. Microbiol. 2018;10(6):441-6. [PMID] [PMCID]
22. Wei B, Mei P, Huang S, Yu X, Zhi T, Wang G, et al. Evaluation of the SureX HPV genotyping test for the detection of high-risk HPV in cervical cancer screening. Virol J. 2020;17(1):1-8. [DOI:10.1186/s12985-020-01417-8] [PMID] [PMCID]
23. Whittle B, Varga C, Berko A, Horvath K, Posa A, Riley J, et al. Attenuation of inflammation and cytokine production in rat colitis by a novel selective inhibitor of leukotriene A4 hydrolase. Br J Pharmacol. 2008;153(5):983-91. [DOI:10.1038/sj.bjp.0707645] [PMID] [PMCID]
24. Gilbert DC, Wakeham K, Langley RE, Vale CL. Increased risk of second cancers at sites associated with HPV after a prior HPV-associated malignancy, a systematic review and meta-analysis. Br J Cancer. 2019;120(2):256-68. [DOI:10.1038/s41416-018-0273-9] [PMID] [PMCID]
25. Lawson J, Glenn W, Heng B, Ye Y, Tran B, Lutze-Mann L, et al. Koilocytes indicate a role for human papilloma virus in breast cancer. Br J Cancer. 2009;101(8):1351-6. [DOI:10.1038/sj.bjc.6605328] [PMID] [PMCID]
26. Farahmand Z, Soleimanjahi H, Garshasbi M, Hasanzadeh M, Zafari E. Distribution of the most common types of HPV in Iranian women with and without cervical cancer. Women Health. 2021;61(1):73-82. [DOI:10.1080/03630242.2020.1822490] [PMID]
27. Perez CE, Chapel DB, Skala SL. Application of current pathologic criteria for atypical placental site nodule suggests that refined criteria are needed. Int J Gynecol Pathol. 2023:10.1097. [DOI:10.1097/PGP.0000000000000934] [PMID]
28. Nategh F, Mohit M, Saatian M, Farahmand Z, Soleimanjahi H. Histopathological characteristics and HPV status in cervical biopsy specimens diagnosed as flat condyloma. Iran J Microbiol. 2023;15(3):468-74. [DOI:10.18502/ijm.v15i3.12909] [PMID] [PMCID]
29. Mohammadnejad E, Manshadi SAD, Mohammadi MTB, Abdollai A, Seifi A, Salehi MR, et al. Prevalence of nosocomial infections in COVID-19 patients admitted to the intensive care unit of Imam Khomeini complex hospital in Tehran. Iran J Microbiol. 2021;13(6):764-8. [DOI:10.18502/ijm.v13i6.8075] [PMID] [PMCID]

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