year 17, Issue 4 (July - August 2023)                   Iran J Med Microbiol 2023, 17(4): 406-413 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Dhari Alewi M, Taha Mohammed A. Molecular Detection of Oral Helicobacter pylori and Its Association with Dental Conditions Among Patients with Helicobacter Infection in Baghdad City. Iran J Med Microbiol 2023; 17 (4) :406-413
1- Department of Preventive Dentistry, College of Dentistry, University of Baghdad, Baghdad, Iraq ,
2- Department of Preventive Dentistry, College of Dentistry, University of Baghdad, Baghdad, Iraq
Abstract:   (394 Views)

Background and Aim: Helicobacter pylori is found in the stomach and the oral cavity, which plays a significant role in oral diseases and recurrent gastric infections. This study aimed to detect the presence of oral H. pylori and investigate its potential association with dental caries and erosion.
Materials and Methods: Saliva and plaque samples were collected from two groups: a study group of 40 H. pylori-infected patients, confirmed by immunological tests, and a control group included 40 subjects who were given negative results after laboratory examination. Molecular detection of H. pylori was performed using the polymerase chain reaction (PCR). Additionally, clinical assessments of dental caries and erosions were conducted.
Results: The study group showed a higher prevalence of H. pylori in saliva (62.5%) compared to dental plaque (45.0%). Among the study group, 70% tested positive for H. pylori by PCR, while 30% tested negative. Dental caries experience (DMFS) was slightly higher in the study group compared to the control group, and significant differences in (DS) and (DMFT) (p<0.05). The prevalence of dental erosion was also higher in the study group compared to the control group.
Conclusion: The presence of H. pylori in the oral cavity represents an important risk factor for dental caries and erosion.

Full-Text [PDF 526 kb]   (168 Downloads) |   |   Full-Text (HTML)  (46 Views)  
Type of Study: Original Research Article | Subject: Oral Microbiology
Received: 2023/05/18 | Accepted: 2023/08/25 | ePublished: 2023/09/27

1. Munther S. An Oral Health Status and Treatment Needed in Relation to Dental Knowledge, Among a Group of Children Attending Preventive Department, College of Dentistry, University of Baghdad. J Baghdad Coll Dent. 2015;27(4):138-42. [DOI:10.12816/0024077]
2. Farquhar DR, Divaris K, Mazul AL, Weissler MC, Zevallos JP, Olshan AF. Poor oral health affects survival in head and neck cancer. Oral Oncol. 2017;73:111-7. [DOI:10.1016/j.oraloncology.2017.08.009] [PMID] [PMCID]
3. Darvishi M, Noori M, Nazer MR, Soleiman-Meigooni S, Forootan M. The Relationship between Helicobacter Pylori and Extra-Gastrointestinal Infections. Iran J Med Microbiol. 2020;14(6):543-65. [DOI:10.30699/ijmm.14.6.543]
4. Miyabayashi H, Furihata K, Shimizu T, Ueno I, Akamatsu T. Influence of Oral Helicobacter pylori on the Success of Eradication Therapy Against Gastric Helicobacter pylori. Helicobacter. 2000;5(1):30-7. [DOI:10.1046/j.1523-5378.2000.00004.x] [PMID]
5. Moghadam MT, Chegini Z, Norouzi A, Dousari AS, Shariati A. Three-Decade Failure to the Eradication of Refractory Helicobacter pylori Infection and Recent Efforts to Eradicate the Infection. Curr Pharm Biotechnol. 2021;22(7):945-59. [DOI:10.2174/1389201021666200807110849] [PMID]
6. Taati Moghadam M, Amirmozafari N, Mojtahedi A, Bakhshayesh B, Shariati A, Masjedian Jazi F. Association of perturbation of oral bacterial with incident of Alzheimer's disease: A pilot study. J Clin Lab Anal. 2022;36(7):e24483. [DOI:10.1002/jcla.24483] [PMID] [PMCID]
7. Moghadam MT, Bakhshayesh B, Babakhani S, Ajorloo P, Shariati A, Mirzaei M, et al. The effect of bacterial composition shifts in the oral microbiota on Alzheimer's disease. Curr Mol Med. 2023. [DOI:10.2174/1566524023666220819140748] [PMID]
8. Moghadam MT, Ramírez-Coronel AA, Darijani S, Akbarizadeh MR, Naderifar M, Soltaninejad S, et al. Perturbations in Microbiota Composition as a Novel Mediator in Neuropsychiatric, Neurological and Mental Disorders: Preventive and Therapeutic Complementary Therapies to Balance the Change. Curr Alzheimer Res. 2023;20(4):213-23. [DOI:10.2174/1567205020666230718160914] [PMID]
9. Abonayla MF, Alwaheb AM. The Effect of Socioeconomic Level on Dental Caries among Preschool Children in Baghdad City. Indian J Public Health. 2020;11(02):2338-42. [DOI:10.37506/v11/i2/2020/ijphrd/195185]
10. Al-Abbasi SW, Radhi NJMH. Dental Caries and Treatment Needs among Kindergarten Children in Al-Basrah Governorate/Iraq. J Baghdad Coll Dent. 2016;28(4):149-52. [DOI:10.12816/0033227]
11. Alshmaa ZM, Alwaheb AM. Impact of Dental Caries on Quality of Life of Primary School Children aged 8-10 Years in Al-Najaf City, Iraq. Indian J Public Health. 2019;10(10):2691-5. [DOI:10.5958/0976-5506.2019.03274.1]
12. Khaudyer AT, Mohammed AT, Al-Ghurabi BH. Oral health condition among Kindergarten children in Relation to Salivary soluble Cluster of Differentiation 14 and Tool Like Receptor 2 (comparative study). Indian J Public Health. 2019;10(9):1407. [DOI:10.5958/0976-5506.2019.02643.3]
13. Schlueter N, Amaechi BT, Bartlett D, Buzalaf MAR, Carvalho TS, Ganss C, et al. Terminology of erosive tooth wear: consensus report of a workshop organized by the ORCA and the Cariology Research Group of the IADR. Caries Res. 2020;54(1):2-6. [DOI:10.1159/000503308] [PMID]
14. Hoobi NM. Caries severity in relation to oral health knowledge and behavior of third and fifth year dental students/University of Baghdad (A comparative study). J Baghdad Coll Dent. 2014;26(3):164-8. [DOI:10.12816/0015244]
15. Heidari K, Kaboosi H, Jamali A, Ghaemi EA, Peyravii Ghadikolaii F. Prevalence of pathogenic genes CagA and VacA of helicobacter pylori isolated in patients with digestive disorders. Iran J Med Microbiol. 2019;13(1):80-8. [DOI:10.30699/ijmm.13.1.80]
16. Mirzaei S, Keshavarzi F, Karami P. The Prevalence of H. Pylori cagA Gene in Patients with Gastric Ulcer. Iran J Med Microbiol. 2021;15(3):345-51. [DOI:10.30699/ijmm.15.3.345]
17. Thamer SR, Al-Ani S. Establishment of the possible association between the presence of Helicobacter pylori in the saliva and gastric biopsy by using polymerase chain reaction technique in association with oral manifestation of peptic ulcer disease. J Baghdad Coll Dent. 2015;27(3):85-8. [DOI:10.12816/0015040]
18. Azami M, Darvishi Z, Borji M, Sayehmiri K. Helicobacter pylori infection is associated with anemia in pregnant women-a meta-analysis study. Iran J Med Microbiol. 2016;10(1):1-7.
19. Iwai K, Watanabe I, Yamamoto T, Kuriyama N, Matsui D, Nomura R, et al. Association between Helicobacter pylori infection and dental pulp reservoirs in Japanese adults. BMC Oral Health. 2019;19(1):267. [DOI:10.1186/s12903-019-0967-2] [PMID] [PMCID]
20. Leimola-Virtanen R, Happonen RP, Syrjänen S. Cytomegalovirus (CMV) and Helicobacter pylori(HP) found in oral mucosal ulcers. J Oral Pathol Med. 1995;24(1):14-7. [DOI:10.1111/j.1600-0714.1995.tb01123.x] [PMID]
21. Rasmussen LT, Labio RWd, Gatti LL, Silva LCd, Queiroz VFd, Smith MdAC, et al. Helicobacter pylori detection in gastric biopsies, saliva and dental plaque of Brazilian dyspeptic patients. Mem Inst Oswaldo Cruz. 2010;105(3):326-30. [DOI:10.1590/S0074-02762010000300015] [PMID]
22. Al-Sarray AJA, Al-Kayat T, Mohammed BM, Al-assadi MJB, Abu-Zaid Y. Dielectric and Electrical Properties of Intercalated 1-(4-nitrophenyl)-N-(p-tolyl) methanimine into the Interlayers of Bentonite Clay. J Med Chem Sci. 2022;5(7(Special Issue)):1321-30.
23. (WHO) WHO. Basic methods of oral health survey. 5 ed. Geneva: World Health Organization; 2013.
24. Lussi A, Jaeggi T. Dental erosion: diagnosis, risk assessment, prevention, treatment: Quintessence; 2011.
25. Khurshid Z, Zohaib S, Najeeb S, Zafar MS, Slowey PD, Almas K. Human saliva collection devices for proteomics: an update. Int J Mol Sci. 2016;17(6):846. [DOI:10.3390/ijms17060846] [PMID] [PMCID]
26. Carvalho SPM, Sales-Peres A, Ribeiro-Bicudo LA, Silva RHAd. Quality evaluation of DNA obtained from stored human saliva and its applicability to identification in Forensic Dentistry. Rev Odonto Cienc. 2010;25:48-53. [DOI:10.1590/S1980-65232010000100010]
27. Valadan Tahbaz S, Yadegar A, Amirmozafari N, Yaghoobee S, Ehsani Ardakani MJ, Zojaji H. Occurrence of Helicobacter pylori and its major virulence genotypes in dental plaque samples of patients with chronic periodontitis in Iran. Gastroenterol Hepatol Bed Bench. 2017;10(Suppl1):S70-S8.
28. Abbaszadegan, M. R., Taghehchian, N., Aarabi, A., Nozari, S., Saburi, E., Moghbeli, M. Kindlin1 As a Sex and Location Specific Diagnostic Marker in Gastric Cancer Patients. Iran J Pathol, 2022; 17(1): 23-28. [DOI:10.30699/ijp.2021.526950.2603] [PMID] [PMCID]
29. RIGGIO MP, LENNON A. Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients. J Med Microbiol. 1999;48(3):317-22. [DOI:10.1099/00222615-48-3-317] [PMID]
30. Jordão HWT, McKenna G, McMenamin ÚC, Kunzmann AT, Murray LJ, Coleman HG. The association between self-reported poor oral health and gastrointestinal cancer risk in the UK Biobank: A large prospective cohort study. United European Gastroenterol J. 2019;7(9):1241-9. [DOI:10.1177/2050640619858043] [PMID] [PMCID]
31. Bürgers R, Schneider-Brachert W, Reischl U, Behr A, Hiller K-A, Lehn N, et al. Helicobacter pylori in human oral cavity and stomach. Eur J Oral Sci. 2008;116(4):297-304. [DOI:10.1111/j.1600-0722.2008.00543.x] [PMID]
32. Yee JKC. Are the view of Helicobacter pylori colonized in the oral cavity an illusion? Exp Mol Med. 2017;49(11):e397. [DOI:10.1038/emm.2017.225] [PMID] [PMCID]
33. Rahman LQ, Rahman AQ, Bakir AA, Muhamadamin RA, Khudhur PK. DNA detection of Helicobacter pylori in saliva of patients with low salivary pH. Zanco j Pure Appl Sci. 2020;24(2):283-90. [DOI:10.15218/zjms.2020.032]
34. Kadota T, Hamada M, Nomura R, Ogaya Y, Okawa R, Uzawa N, et al. Distribution of Helicobacter pylori and periodontopathic bacterial species in the oral cavity. Biomedicines. 2020;8(6):161. [DOI:10.3390/biomedicines8060161] [PMID] [PMCID]
35. Sekhar Goud EVS, Kannan R, Rao UK, Joshua E, Tavaraja R, Jain Y. Identification of Helicobacter pylori in Saliva of Patients with and without Gastritis by Polymerase Chain Reaction. J Pharm Bioallied Sci. 2019;11(Suppl 3):S523-S9. [DOI:10.4103/jpbs.JPBS_260_18] [PMID] [PMCID]
36. Chen Y, Dou G, Wang D, Yang J, Zhang Y, Garnett JA, et al. Comparative Microbial Profiles of Caries and Black Extrinsic Tooth Stain in Primary Dentition. Caries Res. 2021;55(4):310-21. [DOI:10.1159/000517006] [PMID]
37. Hamada M, Nomura R, Ogaya Y, Matayoshi S, Kadota T, Morita Y, et al. Potential involvement of Helicobacter pylori from oral specimens in overweight body-mass index. Sci Rep. 2019;9(1):4845. [DOI:10.1038/s41598-019-41166-5] [PMID] [PMCID]
38. Proctor DM, Relman DA. The landscape ecology and microbiota of the human nose, mouth, and throat. Cell Host Microbe. 2017;21(4):421-32. [DOI:10.1016/j.chom.2017.03.011] [PMID] [PMCID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc