Ghajar Kohestani M, Alipour M, Rezaee A. Cloning & Expression of gene for the vitamin B12 receptor in E.coli O157:H7. Iran J Med Microbiol 2011; 5 (1 and 2) :28-33
URL:
http://ijmm.ir/article-1-178-en.html
1- , Maryam.Qajar@yahoo.com
Abstract: (13865 Views)
Background and objectives: Producing and recognition Vitamin B12 transmitter protein from
Ecoli O157.H7
Material and Methods: btuB gene of Ecoli O157.H7 by using PCR proliferation and in pET28
a (t) vector cloned.
resulted structure confirmed by means of limiting Anzyme Analysis and DNA sequence
determination.
pET28a(+) – btuB transformed in Ecoli Bl21 and recombinant btuB experission with His-Taq
C-terminal performed successfully.
Results: a 66 kiloDoulton Band observed in gell which indicated BtuB protein.
Conclusion: Proliferation btuB gene and producing of BtuB protein with molecular weight 66
kilo Dalton
Type of Study:
Original Research Article |
Subject:
Microbial Biotechnology Received: 2013/11/30 | Accepted: 2013/11/30 | ePublished: 2013/11/30