Detection and Study nan1 and tox A genes of Pseudomonas aeruginosa in Isolates from Otitis Media Patients Considered as Virulence Factors

Mansor, Maryam R.; AL-Khalidi, Zainab Sabah; Almuhanna, Estabraq Hassan; Hussein, Hiba Ridha; Almulla, Abbas F.; Alnaji, Haider Ali

doi:10.30699/ijmm.17.1.81

year 17, Issue 1 (January - February 2023) Iran J Med Microbiol 2023, 17(1): 81-89 | Back to browse issues page

‎ 10.30699/ijmm.17.1.81

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Mansor M R, AL-Khalidi Z S, Almuhanna E H, Hussein H R, Almulla A F, Alnaji H A. Detection and Study nan1 and tox A genes of Pseudomonas aeruginosa in Isolates from Otitis Media Patients Considered as Virulence Factors. Iran J Med Microbiol 2023; 17 (1) :81-89
URL: http://ijmm.ir/article-1-1777-en.html

Detection and Study nan1 and tox A genes of Pseudomonas aeruginosa in Isolates from Otitis Media Patients Considered as Virulence Factors

Maryam R. Mansor¹

, Zainab Sabah AL-Khalidi¹

, Estabraq Hassan Almuhanna¹

, Hiba Ridha Hussein¹

, Abbas F. Almulla

², Haider Ali Alnaji¹

1- Medical Laboratory Technology Department, College of Medical Technology, The Islamic University, Najaf, Iraq
2- Department of Psychiatry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand , abbass.chem.almulla1991@gmail.com

Abstract: (2047 Views)

Background and Aim: Otitis media (OM) or middle ear inflammation is an inflammatory disease caused by a bacterial infection such as Pseudomonas aeruginosa (P. aeruginosa), provided with genetic virulence factors. This study aims to detect nan1 and tox A genes of P. aeruginosa isolated from chronic OM patients.
Materials and Methods: One hundred thirty OM patients were recruited from Al-Sader medical city and Al-Hakim general hospital in Najaf province from September to January 2021 to collect ear swaps. The Primary identification depends on Gram stain and biochemical tests. We also used Vitek 2 system to make another identification and examine resistance to antibiotics. A polymerase chain reaction (PCR) was used to amplify the required genes. Furthermore, gel electrophoresis was performed to show the amplified genes.
Results: The results have revealed that 115 samples (88.46%) give a positive result, while 15 samples (11.54%) are negative for culture bacteria. Only 49 (42.76 %) of the 115 clinical specimens showed positive tests for P. aeruginosa. However, the toxA gene is found in 67.3% of P. aeruginosa isolates, while the nan1 gene is found in 38.77 % of the isolates.
Conclusion: Our findings suggest that P. aeruginosa is one of the most common causes of OM infection in Iraqi patients. Most bacterial isolates show the presence of nan1 and tox A genes, with a high prevalence of tox A than nan1. The presence of these genes is one of the virulence factors and probably partly explains the resistance to antibiotics.

Keywords: nan1, otitis media, Pseudomonas aeruginosa, tox A genes

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Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2022/06/7 | Accepted: 2022/10/8 | ePublished: 2023/01/20

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