year 3, Issue 4 (Winter 2010)
Iran J Med Microbiol 2010, 3(4): 53-60 |
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Parvizi P, Fardid F, Amirkhani A. Isolation process of two genes of wspand 16S rRNAin the intracellular bacterium Wolbachia pipientis in Phlebotomus papatasi sandfly vector of Zoonotic Cutaneous leishmaniasis in Iran. Iran J Med Microbiol 2010; 3 (4) :53-60
URL: http://ijmm.ir/article-1-169-en.html
URL: http://ijmm.ir/article-1-169-en.html
1- Molecular Systematics Laboratoy, Pasteur Institute of Iran,Tehran,Iran. , parp@pasteur.ac.ir
2- Department of Microbiology, Islamic Azad University of Qom,Qom,Iran.
3- Department of Epidemiology, Pasteur Institute of Iran,Tehran,Iran.
2- Department of Microbiology, Islamic Azad University of Qom,Qom,Iran.
3- Department of Epidemiology, Pasteur Institute of Iran,Tehran,Iran.
Abstract: (12000 Views)
Background and Objective: The intracellular Wolbachia pipientisis a Rickettsia-like bacterium. The gene
of W. pipientisis usually congenitally-inherited in insects and can cause cytoplasmic incompatibility (CI),
which is potentially useful for driving genes through populations.
In present study, W. pipientis has been detected in Phlebotomus papatasi, the vector of rural cutaneous
leishmaniosis in Iran by using PCR to amplify fragments either of the 16S ribosomal RNAgene (16S rRNA)
or of the major Wolbachiasurface protein gene wsp.
Materials and Methods: sand flies were screened for the presence of W. pipientis by PCR using the nonstrain specific primers wsp81F with 691R and 16S 99F with 994R. The sequences obtained were edited and aligned using Sequencher TM v. 3.1 to identify W. pipient ishaplo types, which were analysed phylogenetically using PAUP* software.
Results: from 165 Individual wild-caught P. papatasi from Iran, the gene fragment of wspwas found in 124 cases (75.2%), and 16s RNA gene fragment was identified in 123 cases (74.5%). Only one haplotype was obtained for each gene, from which it is inferred that only one A-group genetic strain of W. pipientisoccurs in P. papatasithroughout much of this sandfly's range.
Conclusion: based on the results of this study there is possibility of using just one genetically modified strain of W. pipientisto drive through wild sand fly populations transgenes for intervening in the transmission of L. major by P. papatasi. This genetically modified strain would have to show a CI phenotype in P. papatasi, in order to be spread quickly through wild sand fly populations. Due to natural infection of Wolbachia in sand flies and for their behavior of Cytoplasmic Incompatibility(CI) , Wolbachia can be used as a transferring gene in populations of sand flies to control Leishmaniasis
Materials and Methods: sand flies were screened for the presence of W. pipientis by PCR using the nonstrain specific primers wsp81F with 691R and 16S 99F with 994R. The sequences obtained were edited and aligned using Sequencher TM v. 3.1 to identify W. pipient ishaplo types, which were analysed phylogenetically using PAUP* software.
Results: from 165 Individual wild-caught P. papatasi from Iran, the gene fragment of wspwas found in 124 cases (75.2%), and 16s RNA gene fragment was identified in 123 cases (74.5%). Only one haplotype was obtained for each gene, from which it is inferred that only one A-group genetic strain of W. pipientisoccurs in P. papatasithroughout much of this sandfly's range.
Conclusion: based on the results of this study there is possibility of using just one genetically modified strain of W. pipientisto drive through wild sand fly populations transgenes for intervening in the transmission of L. major by P. papatasi. This genetically modified strain would have to show a CI phenotype in P. papatasi, in order to be spread quickly through wild sand fly populations. Due to natural infection of Wolbachia in sand flies and for their behavior of Cytoplasmic Incompatibility(CI) , Wolbachia can be used as a transferring gene in populations of sand flies to control Leishmaniasis
Type of Study: Original Research Article |
Subject:
Medical Virology
Received: 2013/11/22 | Accepted: 2013/11/22 | ePublished: 2013/11/22
Received: 2013/11/22 | Accepted: 2013/11/22 | ePublished: 2013/11/22
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