year 16, Issue 5 (September - October 2022)                   Iran J Med Microbiol 2022, 16(5): 472-478 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Raina D, Negi A, Pandita A, Rawat N. Prevalence of High Level Aminoglycoside Resistance and Vancomycin Resistance in Enterococcal Isolates at A Tertiary Care Hospital. Iran J Med Microbiol 2022; 16 (5) :472-478
1- Department of Microbiology Shri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun, Uttarakhand, India ,
2- Department of Community medicine, Shri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun, Uttarakhand, India
3- Department of Microbiology Shri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun, Uttarakhand, India
Abstract:   (493 Views)

Background and Aim: Vancomycin resistant Enterococci (VRE) and high level aminoglycoside resistant (HLAR) Enterococci have complicated the available treatment modalities for Enterococci worldwide. The existing study was planned to evaluate the occurrence of HLAR and VRE strains in a tertiary care center in India and to study the association of HLAR with vancomycin sensitive Enterococci (VSE) and VRE.
Materials and Methods: A total of 50 enterococcal isolates from various clinical specimens were incorporated in the study. Speciation was done on the basis of standard biochemical tests. HLAR was tested by the disc diffusion method using 150µg gentamicin disc and 200 µg streptomycin discs. Vancomycin susceptibility patterns were reported using vancomycin disc and agar dilution methods.
Results & Conclusion: Pus samples comprised of the majority for the isolation of enterococcal strains (40%). 54% isolated strains were HLGR, 32% were HLSR and 14% isolates were positive for both HLGR and HLSR. 61.7% of Enterococcus faecium isolates demonstrated resistance for high-level gentamicin (HLGR) and 43.75% Enterococcus faecalis isolates were resistant to high-level streptomycin (HLSR). When VRE was compared to VSE, the rate of HLSR was detected to be 4.64% in VRE, while it was 32.55% in VSE; the rate of HLGR was noted to be 11.62% in VRE and it was 41.87% in VSE. The association of HLGR with HLSR (HLAR) was 2.32% in VRE and 13.95% in VSE strains. Enterococci strains are showing an increase in their antimicrobial resistance patterns. The increment of such strains in health care settings has to be reserved and controlled to avert complicated infections.

Full-Text [PDF 496 kb]   (270 Downloads) |   |   Full-Text (HTML)  (173 Views)  
Type of Study: Brief Original Article | Subject: Antibiotic Resistance
Received: 2022/02/11 | Accepted: 2022/06/4 | ePublished: 2022/08/8

1. Mittal S, Singla P, Deep A, Bala K, Sikka R, Garg M, et al. vancomycin and high level aminogly-coside resistance in Enterococcus spp. in a tertiary health care centre: a therapeutic concern. J Pathog. 2016;2016. [DOI:10.1155/2016/8262561] [PMID] [PMCID]
2. Randhawa VS, Kapoor L, Singh V, Mehta G. Aminoglycoside resistance in enterococci isolated from paediatric septicaemia in a tertiary care hospital in north India. Indian J Med Res. 2004;119:77-9.
3. Baldir G, Engin DO, Kucukercan M, Asuman I, Akçay S, Ozyurek S, et al. High-level resistance to aminoglycoside, vancomycin, and linezolid in enterococci strains. J Microbiol Infect Dis. 2013;3 (03):100-3. [DOI:10.5799/ahinjs.02.2013.03.0090]
4. Biswas P, Dey S, Adhikari L, Sen A. Detection of vancomycin resistance in Enterococcus species isolated from clinical samples and feces of colonized patients by phenotypic and genotypic methods. Indian J Pathol Microbiol. 2016;59(2): 188-93. [DOI:10.4103/0377-4929.182015] [PMID]
5. CLSI. Performance Standards for Anti Microbial susceptibility testing. 31st ed. CLSI Supplement M100: Clinical and Laboratory Standards Institute; 2021.
6. Hemalatha G, Bhaskaran K, Sowmiya M, Anusheela Howlader SK. A study on virulence factors and antimicrobial resistance pattern among enterococci isolated from various clinical specimens from a tertiary care hospital. J Res Med Sci. 2017;5(7):2969-74. [DOI:10.18203/2320-6012.ijrms20172971]
7. Sreeja S, Pr SB, Prathab AG. The prevalence and the characterization of the enterococcus species from various clinical samples in a tertiary care hospital. J Clin Diagnostic Res: JCDR. 2012;6(9): 1486. [DOI:10.7860/JCDR/2012/4560.2539] [PMID] [PMCID]
8. Mendiratta D, Kaur H, Deotale V, Thamke D, Narang R, Narang P. Status of high level aminoglycoside resistant Enterococcus faecium and Enterococcus faecalis in a rural hospital of central India. Indian J Med Microbiol. 2008; 26(4):369. [DOI:10.1016/S0255-0857(21)01817-X] [DOI:10.4103/0255-0857.43582] [PMID]
9. Shah L, Mulla S, Patel KG, Rewadiwala S. Prevalence of enterococci with higher resistance level in a tertiary care hospital: a matter of concern. Indian J Med Res. 2012;2(01):25-7.
10. Agarwal J, Kalyan R, Singh M. High-level aminoglycoside resistance and beta-lactamase production in enterococci at a tertiary care hospital in India. Jpn J Infect Dis. 2009;62(2):158-9.
11. Shrihari N, Kumudini TS, Karadesai SG, Metgud SC. Speciation of enterococcal isolates and antibiotic susceptibility test including high level aminoglycoside resistance and minimum inhibitory concentration for vancomycin. Int J Biol Med Res. 2011;2(4):865-9.
12. Benjamin PH, Peter BW, Patrick GPC, Tony MK, Andrew F, Philipp dC, et al. Treatment Outcomes for Serious Infections Caused by methicillin-resistant Staphylococcus aureus with Reduced vancomycin Susceptibility. Clin Infect Dis. 2004;8(4):521-8. [DOI:10.1086/381202] [PMID]
13. Yaqoob S, Shukla P, Hide F, Parveen Z, Shukla V. Prevalence of vancomycin resistant Enterococcus and its antibiotic resistance pattern in patients admitted in tertiary care hospital. Natl J Med Allied Sci. 2013;2(1):33-40.
14. Verma D, Sinha S, Prakash V. Prevalence and comparison of high-level aminoglycoside resistance in vancomycin-sensitive and vancomycin resistant Enterococcus at a tertiary care hospital in Rohillkhand region. Int J Med Res Rev. 2015;3(10):1162-6. [DOI:10.17511/ijmrr.2015.i10.210]
15. Yangzom T, Singh TSK. Study of vancomycin and high-level aminoglycoside-resistant Enterococcus species and evaluation of a rapid spot test for enterococci from Central Referral Hospital, Sikkim, India. J Lab Physicians. 2019;11 (03):192-9. [DOI:10.4103/JLP.JLP_5_19] [PMID] [PMCID]
16. Khodabandeh M, Mohammadi M, Abdolsalehi MR, Hasannejad-Bibalan M, Gholami M, Alvandimanesh A, et al. High-level aminoglycoside resistance in Enterococcus faecalis and Enterococcus faecium; as a serious threat in hospitals. Infect Disord Drug Targets (Formerly Current Drug Targets-Infectious Disorders). 2020;20(2):223-8. [DOI:10.2174/22123989OTQ5pMTgbTcVY] [DOI:10.2174/1871526519666181130095954] [PMID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc