year 3, Issue 4 (Winter 2010)
Iran J Med Microbiol 2010, 3(4): 1-8 |
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Fazeli H, Moslehi Z, Irajian G, Salehi M. Determination of Drug resistance patterns and detection of bla-VIM gene in Pseudomonas aeruginosastrains Isolated from burned patients in the Emam Mosa Kazem hospital, Esfahan, Iran (2008-9).. Iran J Med Microbiol 2010; 3 (4) :1-8
URL: http://ijmm.ir/article-1-162-en.html
URL: http://ijmm.ir/article-1-162-en.html
1- Department of Microbiology, School of Medicine ,Esfehan University of Medical Sciences.
2- Department of Microbiology, School of Medicine, Iran University of Medical Sciences. ,girajian@yahoo.com
3- Department of Genetic, School of Medicine,Esfehan University of Medical Sciences.
2- Department of Microbiology, School of Medicine, Iran University of Medical Sciences. ,
3- Department of Genetic, School of Medicine,Esfehan University of Medical Sciences.
Abstract: (18938 Views)
Background and abjectives: Pseudomonas aeruginosa is the most common cause of burn wound infections
in many centers. This bacterium shows high resistance to majority of antibiotics including β-lactams. The
frequency of beta-lactam resistant P.aeruginosa is increasing in many countries due to production of
Metallo beta lactamase enzymes. The aim of this study was to determine the antibiotic resistance patterns of
Pseudomonas aeruginosa isolated from burn wound infections and to identify bla-VIM gene by application
of phenotypic and genotypic methods.
Material and Method: 79 isolates of Pseudomonas aeruginosa were recovered from 111 burn patients using microbiological methods. For all the isolates, antibiotic susceptibility tests were performed by Kirby– Bauer disc diffusion method. All imipenem resistant isolates were screened for MBLs production by IPMEDTA disk and in order to detect blaVIM gene, PCR analysis was performed.
Results: Resistance rate of the isolated strains were as fallows: imipenem 94.9% pipracilin 97.4% ciprofloxacin 98.7% tobramycin 95% ceftazidim and ticarcilin 100%. IPM-EDTA disk method showed that 41 out of 74 (55.4%) of imipenem resistant isolates of P. aeruginosa, were metallo-β-lactamase producers. The PCR method showed that 34 out of 79 P. aeruginosaisolates were positive for blaVIM. Conclusion: The results demonstrated that incidence of MBLs producing Pseudomonas aeruginosain burn patients is higher than expected. Therefore detection of antibiotic resistance patterns of bacteria as well as detection of MBLs enzyme producing isolates are of great importance in prevention and control of these infections.
Material and Method: 79 isolates of Pseudomonas aeruginosa were recovered from 111 burn patients using microbiological methods. For all the isolates, antibiotic susceptibility tests were performed by Kirby– Bauer disc diffusion method. All imipenem resistant isolates were screened for MBLs production by IPMEDTA disk and in order to detect blaVIM gene, PCR analysis was performed.
Results: Resistance rate of the isolated strains were as fallows: imipenem 94.9% pipracilin 97.4% ciprofloxacin 98.7% tobramycin 95% ceftazidim and ticarcilin 100%. IPM-EDTA disk method showed that 41 out of 74 (55.4%) of imipenem resistant isolates of P. aeruginosa, were metallo-β-lactamase producers. The PCR method showed that 34 out of 79 P. aeruginosaisolates were positive for blaVIM. Conclusion: The results demonstrated that incidence of MBLs producing Pseudomonas aeruginosain burn patients is higher than expected. Therefore detection of antibiotic resistance patterns of bacteria as well as detection of MBLs enzyme producing isolates are of great importance in prevention and control of these infections.
Type of Study: Original Research Article |
Subject:
Antibiotic Resistance
Received: 2013/11/22 | Accepted: 2013/11/22 | ePublished: 2013/11/22
Received: 2013/11/22 | Accepted: 2013/11/22 | ePublished: 2013/11/22
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