Identification of Carbapenem Resistance Genes in Escherichia coli Isolated from Blattella germanica by Dot Blot Assay in Hamadan Hospitals, Iran – 2018

Seyfi, Bahareh; Hossainpour , Hadi; Kooti, Sara; Azizi Jalilian, Farid

doi:10.30699/ijmm.16.4.357

year 16, Issue 4 (July - August 2022) Iran J Med Microbiol 2022, 16(4): 357-362 | Back to browse issues page

‎ 10.30699/ijmm.16.4.357

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Seyfi B, Hossainpour H, Kooti S, Azizi Jalilian F. Identification of Carbapenem Resistance Genes in Escherichia coli Isolated from Blattella germanica by Dot Blot Assay in Hamadan Hospitals, Iran – 2018. Iran J Med Microbiol 2022; 16 (4) :357-362
URL: http://ijmm.ir/article-1-1617-en.html

Identification of Carbapenem Resistance Genes in Escherichia coli Isolated from Blattella germanica by Dot Blot Assay in Hamadan Hospitals, Iran – 2018

Bahareh Seyfi¹

, Hadi Hossainpour²

, Sara Kooti³

, Farid Azizi Jalilian

⁴

1- Department of Microbiology, Islamic Azad University, Hamadan, Iran
2- Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
3- Behbahan Faculty of Medical Sciences, Behbahan, Iran
4- Department of Virology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran , azizijalilian@yahoo.com

Abstract: (1646 Views)

Background and Objective: Today, one of the problems of health systems is the presence of cockroaches in hospitals as insects that move freely in and out of the hospitals and are infected with pathogenic bacteria. The aim of this study was to identify carbapenem resistance genes in Escherichia coli isolated from Blattella germanica by dot blot assay in Hamadan hospitals in the west of Iran.
Methods: A total of 109 B. germanica from April to September 2018 were collected from ICUs of different hospitals in the Hamadan province, located in western Iran. The B. germanica were identified using reliable taxonomic keys by an expert in the Department of Entomology, insectarium Hamadan University of Medical Sciences, Iran. The antimicrobial susceptibility test was determined by disk diffusion. The dot blot assay was used to identify resistance genes in E. coli isolated from B. germanica.
Results: Out of 109 B. germanica samples collected from ICUs of different hospitals in Hamadan, 31 samples (28.44%) were positive for E. coli. The highest frequency of antibiotic resistance against ampicillin (100%) and the lowest resistance to imipenem was observed in two isolates (6.45%). The frequency of genes among E. coli isolates in B. germanica was as follows: bla _NDM (4 isolates: 3.66%), bla _OXA-48(one isolate: 0.92%), and other studied genes were not observed in any of the strains.
Conclusion: Cockroaches are an important factor in transmitting Enterobacterales and multidrug-resistant (MDR) strains. Therefore, effective preventive and control measures are needed to reduce vector-borne diseases.

Keywords: Antimicrobial drug resistance, Blatella germanica, Dot blot assay, Escherichia coli, German cockroaches

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Type of Study: Original Research Article | Subject: Antibiotic Resistance
Received: 2022/01/7 | Accepted: 2022/03/15 | ePublished: 2022/05/25

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