year 16, Issue 6 (November - December 2022)                   Iran J Med Microbiol 2022, 16(6): 528-536 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sajid S, ur Rahman S, Mohsin M, Sindhu Z U D. Development of an Immune Complex Vaccine against Infectious Bursal Disease Virus and its Potential Response in Poultry Birds. Iran J Med Microbiol 2022; 16 (6) :528-536
1- Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan
2- Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan ,
3- Department of Parasitology, University of Agriculture, Faisalabad, Pakistan
Abstract:   (2543 Views)

Background and Aim: Infectious Bursal Disease is characterized by destroying the Bursa of Fabricius (Primary lymphoid organ), where B cells mature and differentiate. The virus is very stable and resistant to physical and chemical agents, heat, and ultraviolet radiation. Thus, it persists in poultry houses for several weeks after cleaning and disinfection. The present study was designed to develop the IBD immune complex to evaluate immunoprophylactic potential.
Materials and Methods: The infectious bursal disease virus (IBDV) was procured from the infected Bursae collected from the disease outbreak areas of the Faisalabad District, and molecular detection was done through RT-PCR. The inactivated IBD virus was injected into the layer birds to raise egg yolk antibodies (IgY). The eggs were collected, and the yolk containing antibodies were processed to separate IgY through the ammonium sulfate precipitation method. The known quantity of antigen and antibodies were mixed to develop the immune complex (Icx) antigen. The immune response of immune complex IBD antigen was determined in rabbits. The comparative immune response of immune complex IBD antigen and IBD commercial vaccine was made in poultry birds, and the comparative mean antibody titers were determined through factorial analysis. Eighty, one day old, semi-specific pathogen-free (SPF) chickens were kept in four groups. Chickens were bled weekly to evaluate infectious bursal virus antibody titers by I-ELISA. All the groups were challenged with local IBD virus strain on day 28, and the Bursa to bodyweight ratios was compared after being challenged on day 35.
Results: The study revealed that the antibody titers of G-III were significantly higher (P ≤ 0.05) than those of other groups on days 28, 35, and 42. On day 35, the Bursa to the bodyweight of group-III was significantly lower (P ≤ 0.05) than the challenged control group.
Conclusion: The findings showed that the immune complex (Icx) antigen induced a strong and persistent immunogenic response in terms of antibody titer compared to a conventional live vaccine. Icx provoked better protective immunity and protected chickens against the IBD virus challenge and may be considered a substitute for the IBDV vaccine.

Full-Text [PDF 1106 kb]   (680 Downloads) |   |   Full-Text (HTML)  (688 Views)  
Type of Study: Original Research Article | Subject: Microbial Immunology
Received: 2021/09/24 | Accepted: 2022/07/7 | ePublished: 2022/09/9

1. Abedullah A, Maqbool A, Bukhsh K. Issues and economics of poultry production: A case study of Faisalabad, Pakistan. Pak Vet J. 2007;27(1):25-8.
2. Wei Y, Li J, Zheng J, Xu H, Li L, Yu L. Genetic reassortment of infectious bursal disease virus in nature. Biochem Biophys Res Commun. 2006;350(2):277-87. [DOI:10.1016/j.bbrc.2006.09.040] [PMID]
3. Bughio E, Jatoi AS, Memon M, Bughio R, Khoso PA, Khoso ZA, et al. Effect of Age and Route of Administration on the Efficacy of Live Infectious Bursal Disease Vaccines in Broiler. Sarhad J Agric. 2017;33(2):232-9. [DOI:10.17582/journal.sja/2017/]
4. Müller H, Mundt E, Eterradossi N, Islam MR. Current status of vaccines against infectious bursal disease. Avian Pathol. 2012;41(2):133-9. [DOI:10.1080/03079457.2012.661403] [PMID]
5. Fatima M. Strategy to develop alternative to antibiotics using bacterial second messenger 3', 5'cyclic diguanylic acid as an immunostimulator in broiler chicken. 2013.
6. Khan RSA, Sajid S, Habib M, Ali W, Shah MS-u-D, Sarfraz M. History of Gumboro (infectious bursal disease) in Pakistan. Saudi Pharm J. 2017;25(4):453-9. [DOI:10.1016/j.jsps.2017.04.005] [PMID] [PMCID]
7. Sajid S, ur Rahman S, Nayab S, Khan IU, Javeed A. 71. Infectious bursal disease virus cloning and structural protein (VP2) expression in Escherichia coli. Pure appl biol (PAB). 2020;9(1):743-9. [DOI:10.19045/bspab.2020.90080]
8. Zahid B, Aslam A, Tipu Y, Yaqub T, Butt T. Conventional and molecular detection of infectious bursal disease virus in broiler chicken. Pak J Zool. 2016;48(2).
9. Zhao-Hui D, Zhuang-Sen LIN, He-Rui YAO, Yan-Hong GAO, Zhang K, Su-Qing Z, et al. Preparation of artificial antigen and egg yolk-derived immunoglobulin (IgY) of citrinin for enzyme-linked immunosorbent assay. Biomed Environ Sci. 2009;22(3):237-43. [DOI:10.1016/S0895-3988(09)60051-9]
10. Rekha K, Sivasubramanian C, Chung I-M, Thiruvengadam M. Growth and replication of infectious bursal disease virus in the DF-1 cell line and chicken embryo fibroblasts. Biomed Res Int. 2014;2014. [DOI:10.1155/2014/494835] [PMID] [PMCID]
11. Eterradossi N, Saif Y. Infectious bursal disease (gumboro disease). Manual of diagnostic tests and vaccines for terrestrial animals. 12: World Organisation for Animal Health; 2016.
12. Tayade C, Jaiswal TN, Mishra SC, Koti M. L-Arginine stimulates immune response in chickens immunized with intermediate plus strain of infectious bursal disease vaccine. Vaccine. 2006;24(5):552-60. [DOI:10.1016/j.vaccine.2005.08.059] [PMID]
13. Abou El-Fetouh MS, Hafez MH, El-Attar E-SR, El-Agamy ME. Immune-Complex Infectious Bursal Disease Virus versus Live Attenuated Vaccines to Protect SPF Chicken against Very Virulent Virus Challenge. J World Poult Res. 2020;10(4):556-64. [DOI:10.36380/jwpr.2020.63]
14. Haddad EE. A Message from the immune system: a vaccine technology in needs of exploration. Int J Vaccines Vaccin. 2016;2(4):00039. [DOI:10.15406/ijvv.2016.02.00039]
15. Hassanzadeh M, Hassan M, Fard B, Tooluo A. Evaluation of the immunogenisity of immune complex infectious bursal disease vaccine delivered in ovo to embryonated eggs or subcutaneously to day-old chickens. Int J Poult Sci. 2006;5(1):70-4. [DOI:10.3923/ijps.2006.70.74]
16. Naveed A, Rahman SU, Arshad MI, Aslam B. Immune modulatory potential of anti-idiotype antibodies as a surrogate of foot-and-mouth disease virus antigen. Msphere. 2018;3(5):e00522-18. [DOI:10.1128/mSphere.00522-18] [PMID] [PMCID]
17. Schuurhuis DH, van Montfoort N, Ioan-Facsinay A, Jiawan R, Camps M, Nouta J, et al. Immune complex-loaded dendritic cells are superior to soluble immune complexes as antitumor vaccine. J Immunol. 2006;176(8):4573-80. [DOI:10.4049/jimmunol.176.8.4573] [PMID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc