Detection of Methicillin Resistant Staphylococcus aureus (MRSA) by CHROMagar Versus Cefoxitin Disk Diffusion Method

Koupahi, Hossein; Honarmand Jahromy, Sara; Mardani, Masoud; Khodadadi, Ehsan; Rahbar, Mohammad; Eslami, Parisa; Mohammadzadeh Hashtrood, Mona; Saboorian, Roghieh

doi:10.30699/ijmm.17.2.251

year 17, Issue 2 (March - April 2023) Iran J Med Microbiol 2023, 17(2): 251-255 | Back to browse issues page

‎ 10.30699/ijmm.17.2.251

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Koupahi H, Honarmand Jahromy S, Mardani M, Khodadadi E, Rahbar M, Eslami P, et al . Detection of Methicillin Resistant Staphylococcus aureus (MRSA) by CHROMagar Versus Cefoxitin Disk Diffusion Method. Iran J Med Microbiol 2023; 17 (2) :251-255
URL: http://ijmm.ir/article-1-1487-en.html

Detection of Methicillin Resistant Staphylococcus aureus (MRSA) by CHROMagar Versus Cefoxitin Disk Diffusion Method

Hossein Koupahi¹

, Sara Honarmand Jahromy¹

, Masoud Mardani²

, Ehsan Khodadadi³

, Mohammad Rahbar⁴

, Parisa Eslami⁵

, Mona Mohammadzadeh Hashtrood⁶

, Roghieh Saboorian⁶

1- Department of Microbiology, Varamin- Pishva branch, Islamic Azad University, Varamin, Iran
2- Infectious Disease and Tropical Medicine Research Center, Shahid Beheshti Medical University, Tehran, Iran
3- Department of Agronomy and Plant Breeding, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4- Department of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran , rahbar.reflab@gmail.com
5- Department of Microbiology, Central laboratory, Milad Hospital, Tehran, Iran
6- Department of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran

Abstract: (2602 Views)

Background and Aim: MRSA, or methicillin-resistant Staphylococcus aureus, has arisen as a nosocomial and community-acquired infection throughout the world. MRSA identification in the laboratory is difficult for a variety of reasons. The aim of this study was to investigate several phenotypic methods for the detection of MRSA compared with the PCR-based method as the gold standard.
Materials and Methods: A total of 220 clinical isolates of S. aureus were recovered from diverse clinical specimens between August 1, 2019 and June 30, 2020 at Milad Hospital in Tehran, Iran. Cefoxitin discs, CHROMagar™ MRSA medium, and identification of the mecA gene by Polymerase Chain Reaction (PCR) as the gold standard method were used to assess methicillin resistance.
Results and Conclusion: PCR testing revealed that 105 (47.72%) of 220 S. aureus isolates were positive for the mecA gene. The results of the Cefoxitin disc diffusion method showed that it has similar sensitivity and specificity to PCR method. The sensitivity and specificity of CHROMagar™ MRSA medium were both 100%. The Cefoxitin disc diffusion method had the same sensitivity and specificity as the PCR method for detecting the mecA gene. For MRSA detection, the Cefoxitin disc diffusion method can be employed as an alternative to PCR.

Keywords: Methicillin Resistant, mecA gene, Staphylococcus aureus, CHROMagar, MRSA

Full-Text [PDF 526 kb] (803 Downloads) | | Full-Text (HTML) (586 Views)

Type of Study: Brief Original Article | Subject: Medical Bacteriology
Received: 2022/05/15 | Accepted: 2022/11/28 | ePublished: 2023/03/30

References

1. de Carvalho SP, de Almeida JB, Andrade Y, Silva L, Chamon RC, Santos K, et al. Molecular characteristics of methicillin-resistant Staphylococcus aureus isolates from hospital and community environments in northeastern Brazil. Braz J Infect Dis. 2019. [DOI:10.1016/j.bjid.2019.04.005] [PMID] [PMCID]

2. Appelbaum PC. MRSA--the tip of the iceberg. Clin Microbiol Infect. 2006;12 Suppl 2:3-10. [DOI:10.1111/j.1469-0691.2006.01402.x] [PMID]

3. Rahbar M, Karamiyar M, Gra-Agaji R. Nasal carriage of methicillin-resistant Staphylococcus aureus among healthcare workers of an Iranian hospital. Infect Control Hosp Epidemiol. 2003;24(4):236-7. [DOI:10.1086/503478] [PMID]

4. Stefani S, Chung DR, Lindsay JA, Friedrich AW, Kearns AM, Westh H, et al. Meticillin-resistant Staphylococcus aureus (MRSA): global epidemiology and harmonisation of typing methods. Int J Antimicrob Agents. 2012;39(4):273-82. [DOI:10.1016/j.ijantimicag.2011.09.030] [PMID]

5. Askari E, Soleymani F, Arianpoor A, Tabatabai SM, Amini A, Naderinasab M. Epidemiology of mecA-Methicillin Resistant Staphylococcus aureus (MRSA) in Iran: A Systematic Review and Meta-analysis. Iran J Basic Med Sci. 2012;15(5):1010-9.

6. Koupahi H, Honarmand Jahromy S, Rahbar M. Evaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA). Iran J Pathol. 2016;11(4):370-6.

7. Deplano A, Dodémont M, Denis O, Westh H, Gumpert H, Larsen AR, et al. European external quality assessments for identification, molecular typing and characterization of Staphylococcus aureus. J Antimicrob Chemother. 2018;73(10):2662-6. [DOI:10.1093/jac/dky260] [PMID]

8. Mohanasoundaram KM, Lalitha MK. Comparison of phenotypic versus genotypic methods in the detection of methicillin resistance in Staphylococcus aureus. Indian J Med Res. 2008;127(1):78-84.

9. Xu Z, Hou Y, Peters BM, Chen D, Li B, Li L, et al. Chromogenic media for MRSA diagnostics. Mol Biol Rep. 2016;43(11):1205-12. [DOI:10.1007/s11033-016-4062-3] [PMID]

10. Brennan GI, Herra C, Coleman DC, O'Connell B, Shore AC. Evaluation of commercial chromogenic media for the detection of meticillin-resistant Staphylococcus aureus. J Hosp Infect. 2016;92(3):287-92. [DOI:10.1016/j.jhin.2015.10.019] [PMID]

11. Perez-Roth E, Claverie-Martin F, Villar J, Mendez-Alvarez S. Multiplex PCR for simultaneous identification of Staphylococcus aureus and detection of methicillin and mupirocin resistance. J Clin Microbiol. 2001;39(11):4037-41. [DOI:10.1128/JCM.39.11.4037-4041.2001] [PMID] [PMCID]

12. Aghazadeh M, Rahbar M, Monnavar MK, Moghadam FS. Sensitivity pattern of methicillin resistant and methicillin sensitive Staphylococcus aureus isolates, against several antibiotics including tigecycline in Iran: A hospital based study. Pak J Med Sci. 2009;25(3):443-6.

13. Aghamali M, Rahbar M, Kafil HS, Esmailkhani A. Laboratory methods for identification of methicillin-resistant Staphylococcus aureus. Rev Med Microbiol. 2017;28(4):140-51. [DOI:10.1097/MRM.0000000000000116]

14. Pourmand MR, Hassanzadeh S, Mashhadi R, Askari E. Comparison of four diagnostic methods for detection of methicillin resistant Staphylococcus aureus. Iran J Microbiol. 2014;6(5):341-4.

15. Farahani A, Mohajeri P, Gholamine B, Rezaei M, Abbasi H. Comparison of different phenotypic and genotypic methods for the detection of methicillin-resistant Staphylococcus aureus. N Am J Med Sci. 2013;5(11):637-40. [DOI:10.4103/1947-2714.122305] [PMID] [PMCID]

16. Vyas A, Sharma M, Kumar S, Kumar M, Mehra SK. A comparative study of oxacillin screen agar, oxacillin disc diffusion and cefoxitin disc diffusion, oxacillin E-test method for routine screening of methicillin resistant Staphylococcus aureus. Int J Cur Res Rev. 2015;7:55-60.

17. Manickam K, Walkty A, Lagacé-Wiens PR, Adam H, Swan B, McAdam B, et al. Evaluation of MRSASelect™ chromogenic medium for the early detection of methicillin-resistant Staphylococcus aureus from blood cultures. Can J Infect Dis Med Microbiol. 2013;24(4):e113-e6. [DOI:10.1155/2013/201516] [PMID] [PMCID]