Detection of Methicillin Resistant Staphylococcus aureus (MRSA) by CHROMagar Versus Cefoxitin Disk Diffusion Method

Koupahi, Hossein; Honarmand Jahromy, Sara; Mardani, Masoud; Khodadadi, Ehsan; Rahbar, Mohammad; Eslami, Parisa; Mohammadzadeh Hashtrood, Mona; Saboorian, Roghieh

doi:10.30699/ijmm.17.2.251

year 17, Issue 2 (March - April 2023) Iran J Med Microbiol 2023, 17(2): 251-255 | Back to browse issues page

‎ 10.30699/ijmm.17.2.251

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Koupahi H, Honarmand Jahromy S, Mardani M, Khodadadi E, Rahbar M, Eslami P, et al . Detection of Methicillin Resistant Staphylococcus aureus (MRSA) by CHROMagar Versus Cefoxitin Disk Diffusion Method. Iran J Med Microbiol 2023; 17 (2) :251-255
URL: http://ijmm.ir/article-1-1487-en.html

Detection of Methicillin Resistant Staphylococcus aureus (MRSA) by CHROMagar Versus Cefoxitin Disk Diffusion Method

Hossein Koupahi¹

, Sara Honarmand Jahromy¹

, Masoud Mardani²

, Ehsan Khodadadi³

, Mohammad Rahbar

⁴, Parisa Eslami⁵

, Mona Mohammadzadeh Hashtrood⁶

, Roghieh Saboorian⁶

1- Department of Microbiology, Varamin- Pishva branch, Islamic Azad University, Varamin, Iran
2- Infectious Disease and Tropical Medicine Research Center, Shahid Beheshti Medical University, Tehran, Iran
3- Department of Agronomy and Plant Breeding, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4- Department of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran , rahbar.reflab@gmail.com
5- Department of Microbiology, Central laboratory, Milad Hospital, Tehran, Iran
6- Department of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran

Abstract: (1521 Views)

Background and Aim: MRSA, or methicillin-resistant Staphylococcus aureus, has arisen as a nosocomial and community-acquired infection throughout the world. MRSA identification in the laboratory is difficult for a variety of reasons. The aim of this study was to investigate several phenotypic methods for the detection of MRSA compared with the PCR-based method as the gold standard.
Materials and Methods: A total of 220 clinical isolates of S. aureus were recovered from diverse clinical specimens between August 1, 2019 and June 30, 2020 at Milad Hospital in Tehran, Iran. Cefoxitin discs, CHROMagar™ MRSA medium, and identification of the mecA gene by Polymerase Chain Reaction (PCR) as the gold standard method were used to assess methicillin resistance.
Results and Conclusion: PCR testing revealed that 105 (47.72%) of 220 S. aureus isolates were positive for the mecA gene. The results of the Cefoxitin disc diffusion method showed that it has similar sensitivity and specificity to PCR method. The sensitivity and specificity of CHROMagar™ MRSA medium were both 100%. The Cefoxitin disc diffusion method had the same sensitivity and specificity as the PCR method for detecting the mecA gene. For MRSA detection, the Cefoxitin disc diffusion method can be employed as an alternative to PCR.

Keywords: Methicillin Resistant, mecA gene, Staphylococcus aureus, CHROMagar, MRSA

Full-Text [PDF 526 kb] (537 Downloads) | | Full-Text (HTML) (439 Views)

Type of Study: Brief Original Article | Subject: Medical Bacteriology
Received: 2022/05/15 | Accepted: 2022/11/28 | ePublished: 2023/03/30

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