year 3, Issue 1 (Spring 2009)
Iran J Med Microbiol 2009, 3(1): 31-35 |
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Niakan M, Moradi B, Ragheb S. The prevalence of Ureaplasma urealyticumin semen of infertile men Niakan M* , Moradi B, Ragheb SH. Iran J Med Microbiol 2009; 3 (1) :31-35
URL: http://ijmm.ir/article-1-142-en.html
URL: http://ijmm.ir/article-1-142-en.html
1- , niakan@shahed.ac.ir
Abstract: (13379 Views)
Background and Objectives: Infertility is one of the most important problems for every couple.
Micoorganisms, in particular Ureaplasma urealyticum,are among the causative agents of infertility,
although the correlation of this organism and infertility is not quite clear.
The aim of this study was to determine the prevalence of Ureaplasma urealyticumin semen of infertile men.
Material and Method: In this case- control study, presence of Ureaplasma urealyticum was investigated in 40 infertile patients referred to infertility clinic of Rooyan institute. The study group was compared to 40 normal men as a control case group. The prepared questionnaires were filled by both groups. Semen samples were taken and added to PPLO broth containing urea, antibiotics and necessary additives with pH 5.5 as transport medium, and sent to microbiology laboratory. The samples were cultured on PPLO agar then the grown colonies were studied by Dienes staining followed by standard identification tests. The information were analyzed with SPSS software by frequency, chi-square and T-test Method.
Results: The mean age of patient and control groups were 31.87±5.22 and 31.45±5.81 respectively. The rates of isolation of Ureaplasma urealyticum were 11(27.5%) in patients and 4 (10%) in normal controls, which the difference was statistically significant (P< 0.045).
Conclusion: We have shown a significant increase in Ureaplasma urealyticum infection in infertile men. The finding of this study reveals the significant correlation between infertility and Ureaplasma urealyticum infection in patients' group compared to healthy controls.
Material and Method: In this case- control study, presence of Ureaplasma urealyticum was investigated in 40 infertile patients referred to infertility clinic of Rooyan institute. The study group was compared to 40 normal men as a control case group. The prepared questionnaires were filled by both groups. Semen samples were taken and added to PPLO broth containing urea, antibiotics and necessary additives with pH 5.5 as transport medium, and sent to microbiology laboratory. The samples were cultured on PPLO agar then the grown colonies were studied by Dienes staining followed by standard identification tests. The information were analyzed with SPSS software by frequency, chi-square and T-test Method.
Results: The mean age of patient and control groups were 31.87±5.22 and 31.45±5.81 respectively. The rates of isolation of Ureaplasma urealyticum were 11(27.5%) in patients and 4 (10%) in normal controls, which the difference was statistically significant (P< 0.045).
Conclusion: We have shown a significant increase in Ureaplasma urealyticum infection in infertile men. The finding of this study reveals the significant correlation between infertility and Ureaplasma urealyticum infection in patients' group compared to healthy controls.
Type of Study: Original Research Article |
Subject:
Medical Bacteriology
Received: 2013/11/21 | Accepted: 2013/11/21 | ePublished: 2013/11/21
Received: 2013/11/21 | Accepted: 2013/11/21 | ePublished: 2013/11/21
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