Roshdi Maleki M, Moaddab S. Drug susceptibility pattern of Mycobacterium tuberculosisstrains to first and second line drugs in Tabriz, Iran. Iran J Med Microbiol 2009; 3 (1) :18-24
URL:
http://ijmm.ir/article-1-140-en.html
1- Department of Biology, IslamicAzad University, Malekan, Iran , Me2_roshdi@hotmail.com
2- Paramedical Faculty and Research Center for TB and Pulmonary Diseases, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract: (15935 Views)
Background and Objectives: The Number of drug-resistant Mycobacterium tuberculosis strains have
increased significantly since the chemotherapy started against TB. The incidence rates of MDR-TB in
adjacent countries of Iran such as Azerbaijan has been reported in extremely high level. The referring
patients from these countries to Tabriz research center for TB and pulmonary diseases have been raised the
anxiety of contagion risk of drug-resistant TB. The aim of this study was to investigate the in vitro
susceptibility of isolated M. tuberculosis strains to the first and some second line anti-tuberculosis drugs.
Materials and Methods: Initially, 103 M. tuberculosis strains were identified by conventional culture and
biochemical tests. Then drug susceptibility of the strains were investigated by proportional method on
Lowenstein – Jensen medium. H37Rv M. tuberculosis (susceptible to all drugs) was used as a control strain.
The tested drugs were isoniazid, rifampin, ethambutol and streptomycin as first line anti-tuberculosis drugs,
and amikacin, kanamycin, ofloxacin and ciprofloxacin as second line drugs.
Results: Thirteen out of 103 tested strains (12.6%), were resistant to kanamycin, two strains (1.9%) were
resistant to ofloxacin, one strain (1%) was resistant to amikacin and one strain (1%) was resistant to
ciprofloxacin. Among the first line drugs, the highest resistance was observed against streptomycin (7.8 %).
Besides, the strains showed the highest level of resistance against kanamycin (12.6%). The rate of multidrugresistant (MDR) strains was 2.9 % in present study.
Conclusion: The majority of strains were resistant to all first line drugs and the rate of MDR was low in this
study. Among the first and second line drugs, the highest rates of resistance were observed against
streptomycin and kanamycin respectively.
Type of Study:
Original Research Article |
Subject:
Antibiotic Resistance Received: 2013/11/21 | Accepted: 2013/11/21 | ePublished: 2013/11/21