Iranian Journal of Medical Microbiology

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Hosain Zadegan H, Mohammadi M, Pajohy N, Ebrahim Zade F. Study of lytic phage and alkaline phosphatase on treatment of burn infections caused by Escherichia coliin Mouse . Iran J Med Microbiol 2008; 2 (1) :37-43
URL: http://ijmm.ir/article-1-111-en.html

Background and objectives: Bacteriophages are microorganisms that have been using bacterial hosts for propagation and life cycle. Some of them are called lytic bacteriophages, that lyse bacterial hosts after growth. These kinds of bacteriophages are used for treatment of bacterial infections and phage typing.lytic bacteriophages have several advantages as a treatment against infections in contrast with antibiotics. Therapeutic effects of lytic phage isolated from natural habitates were studied against burn infection of Escherichia coli.
Material and Methods: lytic bacteriophages were isolated from environmental resources using luria broth and overlay method. Then phages propagated using Escherichia colias host, supernatant filtered, and after precipitation of polysaccharides used for treatment of Escherichia coliinfections. For treatment experiments induced burn infections in laboratory mouse were used.
Results: lytic bacteriophages were isolated frequently from human and sheep stools, and sewage. Phage titer isolated from sheep's stool was higher than other samples. Х² analyses results indicate that there was significant difference in death incidence of studied groups.
Conclusion:Based on statistical analysis using lytic bacteriophage for treatment of burn infection of Escherichia coli inhibited 80 % of mouse from death. Result semphesis the potential of bacteriophages as potent antibacterial treatments.