High Sensitive Detection of Staphylococcal Enterotoxin B by Silver Enhanced Lateral Flow Assay

Gholamzad, Mehrdad; Seyedkhan, Zeynab; Morovati Khamsi, Hassan; Khorshidi, Mehdi

doi:10.30699/ijmm.14.4.307

year 14, Issue 4 (July - August 2020) Iran J Med Microbiol 2020, 14(4): 307-313 | Back to browse issues page

‎ 10.30699/ijmm.14.4.307

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Gholamzad M, Seyedkhan Z, Morovati Khamsi H, Khorshidi M. High Sensitive Detection of Staphylococcal Enterotoxin B by Silver Enhanced Lateral Flow Assay. Iran J Med Microbiol 2020; 14 (4) :307-313
URL: http://ijmm.ir/article-1-1066-en.html

High Sensitive Detection of Staphylococcal Enterotoxin B by Silver Enhanced Lateral Flow Assay

Mehrdad Gholamzad¹

, Zeynab Seyedkhan²

, Hassan Morovati Khamsi³

, Mehdi Khorshidi

⁴

1- Department of Microbiology and Immunology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Biology, College of Basic Science, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
3- Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
4- Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran , mehdikhorshidi@yahoo.com

Abstract: (5329 Views)

Background: Staphylococcus aureus is one of the most important microorganisms that can colonize the human body and cause various types of human diseases by secreting virulence factors. The lateral flow assay (LFA) is one of the well-known commercial immunoassay methods that provides a high sensitive and rapid approach to monitoring infectious agents in blood, serum and urine. LFA has been considered as an ideal immunochromatographic test.
Materials & Methods: Anti Staphylococcal Enterotoxin B (SEB) antibodies were conjugated to 20 nm colloidal gold nanoparticles and were applied in assembled lateral flow layer. Suitable reagents were prepared and used in silver enhancement method. We designed a single immunochromatographic test strip to detect SEB.
Results: In this study, the smallest amount of SEB identified using sandwich LFA method was 10 ng/mL. We also established a "silver enhanced method". Silver could improve the sensitivity detection of the test 100-fold greater than the previously mentioned sandwich LFA.
Conclusion: Regarding the high sensitivity of the new method for detection and measurement of SEB (0.1 ng/mL), this strip test offers great promise for a rapid technique instead of the other diagnostic SEB tests in laboratories for the first time.

Keywords: Immunochromatographic test strip, Lateral flow assay, Rapid detection, Silver Enhancement, Staphylococcal enterotoxin B, Test strip

Full-Text [PDF 592 kb] (1089 Downloads) | | Full-Text (HTML) (1680 Views)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2020/02/23 | Accepted: 2020/07/19 | ePublished: 2020/07/20

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