year 3, Issue 4 (Winter 2010)
Iran J Med Microbiol 2010, 3(4): 15-23 |
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Bayat M, Nowroozi J, Pakzad P, Farnia P, Anoosheh S, Marashian S M, et al . Study of TNF-α& IFN-γconcentratin levels of serum in TB patients & control healthy groups by ELISA & evaluation of their polymorphisms by PCR-RFLP. Iran J Med Microbiol 2010; 3 (4) :15-23
URL: http://ijmm.ir/article-1-164-en.html
URL: http://ijmm.ir/article-1-164-en.html
Mahdiye Bayat1 
, Jamileh Nowroozi2 , Parviz Pakzad2 , Parisa Farnia3 , Saber Anoosheh3 , Seyed Mehran Marashian3 , Mohhamad Varahram3 , Mahdi Kazempour3 , Mohammad Reza Masjedi3 , AliAkbar Velayati3
, Jamileh Nowroozi2 , Parviz Pakzad2 , Parisa Farnia3 , Saber Anoosheh3 , Seyed Mehran Marashian3 , Mohhamad Varahram3 , Mahdi Kazempour3 , Mohammad Reza Masjedi3 , AliAkbar Velayati3
1- Department of Microbiology ,Islamic Azad University, Tehran North Branch , kavoshgroup597@yahoo.com
2- Mycobacterium Research Center (MRC), National Research Institute Of Tuberculosis and Lung Diasease (NRITLD), Darabad, Tehran
3- Department of Microbiology ,Islamic Azad University, Tehran North Branch
2- Mycobacterium Research Center (MRC), National Research Institute Of Tuberculosis and Lung Diasease (NRITLD), Darabad, Tehran
3- Department of Microbiology ,Islamic Azad University, Tehran North Branch
Abstract: (15819 Views)
Background & Objectives: Cellular resistance to tuberculosis (TB) infection depends on signals from
mycobacterium tuberculosis specific T lymphocytes activating mycobacterial killing mechanisms in infected
macrophages. The network of soluble factors, or cytokines, responsible for these cellular communications
has been progressively unraveled over the last 3 decades however, new regulatory and effectors cytokines
continue to be discovered. Infection of a host with a pathogen first result inactivation of cells of the innate
immune response including effectors molecules including cytokines such as TNF-α and IFN-γ. The aim of
this study was to investigate the frequency of TNF-α, IFN-γ alleles , evaluating serum concentration of
TNF-α and IFN-γ and relationship of between susceptibility to TB and TNF-α and IFN-γ gene variations.
Material & Methods: In this prospective case-control study, 93 patients with smear positive tuberculosis selected from Masih Daneshvari Hospital, Tehran, Iran. They were matched with 103 controls without any history TB. Genotype of 5 regions of TNF-α and 1 region of IFN-γ were distinguished by PCR-RFLP method, and level of serum concentration between case & control groups were evaluated by ELISA method. Data were analyzed with Mann-Whitney U & earning the cut off analyzed with ROC curve for ELISA method and the results of PCR-RFLP method were analyzed by SPSS, Fisher exact and X2.
Results: In PCR-RFLP method, the results showed a significant difference at TNF-308 and TNF-857 between two groups of control and patient (P< 0.05). In ELISA method, a significant difference in IFN-γ was observed between the groups of control and patient (P <0.05). Also a cut off point as a serologic marker between the positive and negative states, for rapid TB examination about IFN-γwas found the cut off for IFN-γ was 0.19.
Conclusion: Mutation in TNF-308 and TNF-857 regions were identified significantly, but it was not observed in other TNF-α and IFN-γ regions. In this study, ELISA method suggest that mycobacterial pathogens are frequently associated with production of cytokines such as IFN-γ and serologic tests considering the cut off patients, help us to detect TB in cases that we want to earn results rapidly.
Material & Methods: In this prospective case-control study, 93 patients with smear positive tuberculosis selected from Masih Daneshvari Hospital, Tehran, Iran. They were matched with 103 controls without any history TB. Genotype of 5 regions of TNF-α and 1 region of IFN-γ were distinguished by PCR-RFLP method, and level of serum concentration between case & control groups were evaluated by ELISA method. Data were analyzed with Mann-Whitney U & earning the cut off analyzed with ROC curve for ELISA method and the results of PCR-RFLP method were analyzed by SPSS, Fisher exact and X2.
Results: In PCR-RFLP method, the results showed a significant difference at TNF-308 and TNF-857 between two groups of control and patient (P< 0.05). In ELISA method, a significant difference in IFN-γ was observed between the groups of control and patient (P <0.05). Also a cut off point as a serologic marker between the positive and negative states, for rapid TB examination about IFN-γwas found the cut off for IFN-γ was 0.19.
Conclusion: Mutation in TNF-308 and TNF-857 regions were identified significantly, but it was not observed in other TNF-α and IFN-γ regions. In this study, ELISA method suggest that mycobacterial pathogens are frequently associated with production of cytokines such as IFN-γ and serologic tests considering the cut off patients, help us to detect TB in cases that we want to earn results rapidly.
Type of Study: Original Research Article |
Subject:
Molecular Microbiology
Received: 2013/11/22 | Accepted: 2013/11/22 | ePublished: 2013/11/22
Received: 2013/11/22 | Accepted: 2013/11/22 | ePublished: 2013/11/22
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