year 9, Issue 1 (Spring 2015)                   Iran J Med Microbiol 2015, 9(1): 6-13 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Alizade H, Ghanbarpour R, Aflatoonian M R, Sobhanipour M H. Determination of phylogenetic groups and antibiotic resistance pattern of Enterotoxigenic Escherishia coli isolates from diarrheoic cases in Bam City by PCR. Iran J Med Microbiol. 2015; 9 (1) :6-13
URL: http://ijmm.ir/article-1-379-en.html
1- Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences & Zoonosis Research Committee, Kerman University of Medical Sciences, Kerman, Iran
2- Molecular Microbiology Department, Faculty of Veterinary Medicine, Shahid Bahonar University & Zoonosis Research Committee, Kerman University of Medical Sciences, Kerman, Iran
3- Zoonosis Research Committee, Kerman University of Medical Sciences & Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran , mraflatoonian@yahoo.com
4- Department of Microbiology, Kerman University of Medical Sciences, Kerman, Iran
Abstract:   (11643 Views)

Background and Aim: Purposes of this study were to determine the phylogenetic groups, prevalence of enterotoxigenic pathotype and antibiotic resistance of Escherichia coli (E. coli) isolates from diarrheic cases in Bam city.

Materials and Methods: In this study 155 E. coli were isolated from diarrheic samples in Bam city. Phylogenetic groups of isolates and enterotoxigenic pathotype were determined by detection of chuA, yjaA, TspE4C2 and ST, LT genes respectively.

Results: One hundred fifty five examined isolates were distributed in phylogenetic groups: A (71.60%), B1 (3.22%), B2 (9.67%) and D (15.48%). The genes for enterotoxigenic pathotype were detected in 52 isolates (33.54%), which ST gene were found in 29 isolates, LT in 16 isolates and LT, ST genes in 7 isolates. Twenty nine ST gene positive isolates were distributed in three phylogenetic groups A (48.28%), D (41.38%) and B2 (10.34%). According to the antibiotic susceptibility tests maximum and minimum antibiotic resistance rate was against to trimethoprim/sulfamethoxazole (74.19%) and ciprofloxacin and gentamycin (9.67%). Fifteen multiple antibiotic resistance patterns were detected in four phylogenetic groups.

Conclusions: Escherichia coli isolates from enterotoxigenic pathotype have a considerable antibiotic resistance rate in Bam city and were distributed in different phylogenetic groups. Since a considerable number of isolates were negative for LT and ST genes, it is necessary to study the other virulence genes and their phylogenetic background in E. coli isolates from diarrheic cases in Bam city.

Full-Text [PDF 507 kb]   (2575 Downloads)    
Type of Study: Original | Subject: Medical Bacteriology
Received: 2014/11/18 | Accepted: 2014/11/19 | ePublished: 2014/11/23

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2020 All Rights Reserved | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc