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Isolation and characterization of a bacteriophage targeting antibiotic resistant Helicobacter pylori
1- Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
2- Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran ,a.elikaei@alzahra.ac.ir
2- Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran ,
Abstract: (25 Views)
Background and Objective: Novel therapeutic strategies are being considered due to the growing prevalence of antibiotic-resistant Helicobacter pylori. The aim of this study is a bacteriophage against antibiotic-resistant strains of H. pylori isolated from gastric biopsies of patients.
Methods: Untreated wastewater mixed together from Imam Hossein, Taleghani, and Mofid Children’s hospitals. The presence of bacteriophage was confirmed by the double-layer agar technique. Transmission electron microscope (TEM) was used to determine the morphology of the isolated phage. In addition, stability tests were evaluated. Moreover, bacteriophage host range and multiplicity of infection (MOI) were determined. Finally, the phenol-chloroform method was performed to extract the phage genome, and its concentration was calculated by nanodrop.
Results:TEM micrograph showed that the isolated phage belonged to theCaudoviricetes class. It had a wide host range and was effective against 100% of clinical strains of H. pylori and the standard strain of Campylobacter jejuni. Optimal activity for this phage occurred between pH 4 and 7 at 37°C. This phage was sensitive to chloroform and the number of phages increased with the increase in salt concentration. It was revealed that MOI=1 was the optimal MOI for this bacteriophage activity and the extracted DNA concentration was 183 ng/µl.
Conclusion: It is concluded that the isolated phage was effective on the H. pylori strains and can be considered a suitable candidate for further studies.
Methods: Untreated wastewater mixed together from Imam Hossein, Taleghani, and Mofid Children’s hospitals. The presence of bacteriophage was confirmed by the double-layer agar technique. Transmission electron microscope (TEM) was used to determine the morphology of the isolated phage. In addition, stability tests were evaluated. Moreover, bacteriophage host range and multiplicity of infection (MOI) were determined. Finally, the phenol-chloroform method was performed to extract the phage genome, and its concentration was calculated by nanodrop.
Results:
Conclusion: It is concluded that the isolated phage was effective on the H. pylori strains and can be considered a suitable candidate for further studies.
Keywords: Antibiotic resistance, Campylobacter jejuni, Caudoviricetes, H. pylori, Phage therapy, Podoviridae
Type of Study: Original Research Article |
Subject:
Medical Virology
Received: 2025/02/8 | Accepted: 2025/11/1
Received: 2025/02/8 | Accepted: 2025/11/1
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Iranian Journal of Medical Microbiology by Farname is licensed under CC BY-NC 4.0
