Soleimani Rahbar A, Golshani M, Fayyaz F, Rafiee Tabatabaei S, Moradi A. Detection of Mycoplasma DNA from the sperm specimens of infertile men by PCR . Iran J Med Microbiol 2007; 1 (1) :47-53
URL:
http://ijmm.ir/article-1-73-en.html
1- Pediatric Infectious Research Center, Faculty of medicine,Shaheed Beheshti Medical University ,Tehran ,Iran , pedircorg@yahoo.com
2- Faculty of medicine,Shaheed Beheshti Medical University ,Tehran ,Iran
3- Pediatric Infectious Research Center, Shaheed Beheshti University of medical, sciences,Tehran, Iran
Abstract: (20963 Views)
Background and Objectives: Infections in accessory sex glands are considered as potential hazards to male
fertility.These infections can affect different sites of the male reproductive tract such as the testis,
epididymis and male accessory sex glands.Transmission of these infections to female partners causes genital
infection, infertility and abortion. The aim of this study was to detect Mycoplasma, as one of the causatives,
from the semen of infertile males using PCR assay.
Material and methods: The survey considered 100 infertile men who referred to clinics and had not used
antibiotics for 7 days prior to sampling. The infertilityof cases was confirmed by a physician specialist. The
sperm specimens were collected in sterile condition and sent to the laboratory rapidly. Specimens were
examined for presence of Ureaplasma urealiticumand Mycoplasma hominisby PCR. Meanwhile, the
history of vaginal infections and abortion in the female sexual partners was investigated. The results of
multiplex PCR were compared with spermogram. All patients had no symptoms of genital infection.
Results: Of 100 infertile men , 33 (33%) were positive for CMU organisms (Chlamydia, Mycoplasmaand
Uureaplasma). Uureaplasma urealiticumand Mycoplasma hominiswere detected in 17 and 3 of patients
respectively.
Conclusion: Due to some problems in culturing of CMU organisms, PCR can be used as a diagnostic
technique to detect such pathogens from seminal fluid of infertile men that leads to choose appropriate
therapy in a shortest time.
Type of Study:
Original Research Article |
Subject:
Molecular Microbiology Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10