year 1, Issue 1 (Spring 2007)
Iran J Med Microbiol 2007, 1(1): 47-53 |
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Soleimani Rahbar A, Golshani M, Fayyaz F, Rafiee Tabatabaei S, Moradi A. Detection of Mycoplasma DNA from the sperm specimens of infertile men by PCR . Iran J Med Microbiol 2007; 1 (1) :47-53
URL: http://ijmm.ir/article-1-73-en.html
URL: http://ijmm.ir/article-1-73-en.html
AliAkbar Soleimani Rahbar 
1, Mayam Golshani2 , Fariba Fayyaz2 , Sedighe Rafiee Tabatabaei3 , Arezoo Moradi3
1, Mayam Golshani2 , Fariba Fayyaz2 , Sedighe Rafiee Tabatabaei3 , Arezoo Moradi3
1- Pediatric Infectious Research Center, Faculty of medicine,Shaheed Beheshti Medical University ,Tehran ,Iran , pedircorg@yahoo.com
2- Faculty of medicine,Shaheed Beheshti Medical University ,Tehran ,Iran
3- Pediatric Infectious Research Center, Shaheed Beheshti University of medical, sciences,Tehran, Iran
2- Faculty of medicine,Shaheed Beheshti Medical University ,Tehran ,Iran
3- Pediatric Infectious Research Center, Shaheed Beheshti University of medical, sciences,Tehran, Iran
Abstract: (20807 Views)
Background and Objectives: Infections in accessory sex glands are considered as potential hazards to male
fertility.These infections can affect different sites of the male reproductive tract such as the testis,
epididymis and male accessory sex glands.Transmission of these infections to female partners causes genital
infection, infertility and abortion. The aim of this study was to detect Mycoplasma, as one of the causatives,
from the semen of infertile males using PCR assay.
Material and methods: The survey considered 100 infertile men who referred to clinics and had not used antibiotics for 7 days prior to sampling. The infertilityof cases was confirmed by a physician specialist. The sperm specimens were collected in sterile condition and sent to the laboratory rapidly. Specimens were examined for presence of Ureaplasma urealiticumand Mycoplasma hominisby PCR. Meanwhile, the history of vaginal infections and abortion in the female sexual partners was investigated. The results of multiplex PCR were compared with spermogram. All patients had no symptoms of genital infection.
Results: Of 100 infertile men , 33 (33%) were positive for CMU organisms (Chlamydia, Mycoplasmaand Uureaplasma). Uureaplasma urealiticumand Mycoplasma hominiswere detected in 17 and 3 of patients respectively.
Conclusion: Due to some problems in culturing of CMU organisms, PCR can be used as a diagnostic technique to detect such pathogens from seminal fluid of infertile men that leads to choose appropriate therapy in a shortest time.
Material and methods: The survey considered 100 infertile men who referred to clinics and had not used antibiotics for 7 days prior to sampling. The infertilityof cases was confirmed by a physician specialist. The sperm specimens were collected in sterile condition and sent to the laboratory rapidly. Specimens were examined for presence of Ureaplasma urealiticumand Mycoplasma hominisby PCR. Meanwhile, the history of vaginal infections and abortion in the female sexual partners was investigated. The results of multiplex PCR were compared with spermogram. All patients had no symptoms of genital infection.
Results: Of 100 infertile men , 33 (33%) were positive for CMU organisms (Chlamydia, Mycoplasmaand Uureaplasma). Uureaplasma urealiticumand Mycoplasma hominiswere detected in 17 and 3 of patients respectively.
Conclusion: Due to some problems in culturing of CMU organisms, PCR can be used as a diagnostic technique to detect such pathogens from seminal fluid of infertile men that leads to choose appropriate therapy in a shortest time.
Type of Study: Original Research Article |
Subject:
Molecular Microbiology
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10
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