Evaluation of the Expression of NLRP1 Inflammasome in Patients with Bacterial Septicemia

Mousahasankhani, Hamid; Kariminik, Ashraf

doi:10.30699/ijmm.14.3.201

year 14, Issue 3 (May - Jun 2020) Iran J Med Microbiol 2020, 14(3): 201-212 | Back to browse issues page

‎ 10.30699/ijmm.14.3.201

Mendeley

Zotero

RefWorks

Mousahasankhani H, Kariminik A. Evaluation of the Expression of NLRP1 Inflammasome in Patients with Bacterial Septicemia. Iran J Med Microbiol 2020; 14 (3) :201-212
URL: http://ijmm.ir/article-1-1068-en.html

Evaluation of the Expression of NLRP1 Inflammasome in Patients with Bacterial Septicemia

Hamid Mousahasankhani¹

, Ashraf Kariminik

1- Department of Microbiology, Faculty of Science, Kerman Branch, Islamic Azad University, Kerman, Iran
2- Department of Microbiology, Faculty of Science, Kerman Branch, Islamic Azad University, Kerman, Iran , a.kariminik@iauk.ac.ir

Abstract: (4564 Views)

Background: Septicemia is the most important cause of mortality, especially in hospitalized patients, due to the influence of the immune response by infection. NLRP1 (Nod-like receptor P1) is an intracellular receptor that recognizes microbial-dependent molecular patterns. The main intracellular mechanism of anti-septicemia is still being investigated. The purpose of this study was to evaluate the expression of NLRP1 genes in patients with septicemia compared to healthy controls.
Materials & Methods: This cross sectional study was done on 40 blood samples in patient with septicemia and 40 healthy controls using quota sampling. Bacterial species were identified by microbial culture. NLRP1 gene expression was evaluated using Real Time PCR technique.
Results: Four bacteria such as Escherichia coli, Staphylococcus aureus, Acinetobacter baumannii and Pseudomonas aeruginosa reported for causes of septicemia. The results also showed that the expression of NLRP1 inflammasome at mRNA level was significantly increased in patients with septicemia compared to healthy controls. NLRP1 gene expression was not different among patients with different bacterial infections.
Conclusion: NLRP1 appears to be an important receptor against bacteria during bacterial bloodstream infection, and further research, particularly in reducing the expression level of NLRP1 molecules, may play a key role in blood decontamination.

Keywords: Septicemia, NLRP1 (Nod-like receptor P1), gene expression

Full-Text [PDF 1005 kb] (1444 Downloads) | | Full-Text (HTML) (1170 Views)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2020/02/28 | Accepted: 2020/06/16 | ePublished: 2020/05/12

References

1. Hall MJ, Williams SN, DeFrances CJ, Golosinskiy A. Inpatient care for septicemia or sepsis: a challenge for patients and hospitals. NCHS data brief. 2011 ;( 62): 1-8.

2. Blomberg B, Jureen R, Manji KP, Tamim BS, Mwakagile DS, Urassa WK, et al. High rate of fatal cases of pediatric septicemia caused by gram-negative bacteria with extended-spectrum beta-lactamases in Dar es Salaam, Tanzania. Journal of clinical microbiology. 2005; 43(2):745-9. [DOI:10.1128/JCM.43.2.745-749.2005] [PMID] [PMCID]

3. Jawad I, Lukšić I, Rafnsson SB. Assessing available information on the burden of sepsis: global estimates of incidence, prevalence and mortality. Journal of global health. 2012; 2(1):1-9. [DOI:10.7189/jogh.01.010404]

4. Nnanna II, Ehis OJ, Sidiquo II, Nnanna IG, Adekunle O. Serum procalcitonin: Early detection of neonatal bacteremia and septicemia in a tertiary healthcare facility. North American journal of medical sciences. 2011;3(3):157-160. [DOI:10.4297/najms.2011.3157] [PMID] [PMCID]

5. Friedland J, Suputtamongkol Y, Remick D, Chaowagul W, Strieter R, Kunkel S, et al. Prolonged elevation of interleukin-8 and interleukin-6 concentrations in plasma and of leukocyte interleukin-8 mRNA levels during septicemic and localized Pseudomonas pseudomallei infection. Infection and immunity. 1992;60(6):2402-8. [DOI:10.1128/IAI.60.6.2402-2408.1992] [PMID] [PMCID]

6. Chavarría‐Smith J, Vance RE. The NLRP 1 inflammasomes. Immunological reviews. 2015;265(1):22-34. [DOI:10.1111/imr.12283] [PMID]

7. Proell M, Gerlic M, Mace PD, Reed JC, Riedl SJ. The CARD plays a critical role in ASC foci formation and inflammasome signalling. Biochemical Journal. 2013;449(3):613-21. [DOI:10.1042/BJ20121198] [PMID] [PMCID]

8. Winn WC. Koneman's color atlas and textbook of diagnostic microbiology: Lippincott williams & wilkins. 2006.

9. Kariminik A, Yaghobi R, Dabiri S. CXCL9 expression and polyomavirus BK infectivity in renal transplant patients with nephropathy. Cellular and Molecular Biology. 2016;62(1):104-8.

10. Kariminik A, Dabiri S, Yaghobi R. Polyomavirus BK induces inflammation via up-regulation of CXCL10 at translation levels in renal transplant patients with nephropathy. Inflammation. 2016;39(4):1514-9. [DOI:10.1007/s10753-016-0385-4] [PMID]

11. Kariminik A, Yaghobi R, Dabiri S. Association of BK Virus Infection with CXCL11 Gene Expression and Protein Levels in Kidney Transplant Patients. Experimental and clinical transplantation: official journal of the Middle East Society for Organ Transplantation. 2018;16(1):50-4.

12. Haddy RI, Richmond BW, Trapse FM, Fannin KZ, Ramirez JA. Septicemia in patients with AIDS admitted to a university health system: a case series of eighty-three patients. J Am Board Fam Med. 2012;25(3):318-22. [DOI:10.3122/jabfm.2012.03.110106] [PMID]

13. Mehta M, Dutta P, Gupta V. Antimicrobial susceptibility pattern of blood isolates from a teaching hospital in North India. Japanese journal of infectious diseases. 2005;58(3):174-76.

14. Millar BC, Jiru X, Moore JE, Earle JA. A simple and sensitive method to extract bacterial, yeast and fungal DNA from blood culture material. Journal of Microbiological Methods. 2000;42(2):139-47. [DOI:10.1016/S0167-7012(00)00174-3]

15. Kurt B, Flynn P, Shenep JL, Pounds S, Lensing S, Ribeiro RC, et al. Prophylactic antibiotics reduce morbidity due to septicemia during intensive treatment for pediatric acute myeloid leukemia. Cancer. 2008;113(2):376-82. [DOI:10.1002/cncr.23563] [PMID]

16. Nwadioha S, Nwokedi E, Kashibu E, Odimayo M, Okwori E. A review of bacterial isolates in blood cultures of children with suspected septicemia in a Nigerian tertiary Hospital. African Journal of Microbiology Research. 2010;4(4):222-5.

17. Paglia MG, D'Arezzo S, Festa A, Del Borgo C, Loiacono L, Antinori A, et al. Yersinia pseudotuberculosis septicemia and HIV. Emerging infectious diseases. 2005;11(7):1128-30. [DOI:10.3201/eid1107.041268] [PMID] [PMCID]

18. Dabiri S, Kariminik A, Kennedy D. The role of CXCR3 and its ligands in renal transplant outcome. European cytokine network. 2016;27(2):34-40. [DOI:10.1684/ecn.2016.0375] [PMID]

19. Chi W, Li F, Chen H, Wang Y, Zhu Y, Yang X, et al. Caspase-8 promotes NLRP1/NLRP3 inflammasome activation and IL-1β production in acute glaucoma. Proceedings of the National Academy of Sciences. 2014;111(30):11181-6. [DOI:10.1073/pnas.1402819111] [PMID] [PMCID]

20. Saresella M, La Rosa F, Piancone F, Zoppis M, Marventano I, Calabrese E, et al. The NLRP3 and NLRP1 inflammasomes are activated in Alzheimer's disease. Molecular neurodegeneration. 2016;11(23):1-14. [DOI:10.1186/s13024-016-0088-1] [PMID] [PMCID]

21. Shimada K, Crother TR, Arditi M. Innate immune responses to Chlamydia pneumoniae infection: role of TLRs, NLRs, and the inflammasome. Microbes and infection. 2012;14(14):1301-7. [DOI:10.1016/j.micinf.2012.08.004] [PMID] [PMCID]

22. Leemans JC, Cassel SL, Sutterwala FS. Sensing damage by the NLRP3 inflammasome. Immunological reviews. 2011;243(1):152-62. [DOI:10.1111/j.1600-065X.2011.01043.x] [PMID] [PMCID]

23. Kanneganti T-D. Central roles of NLRs and inflammasomes in viral infection. Nature Reviews Immunology. 2010;10(10):688-98. [DOI:10.1038/nri2851] [PMID] [PMCID]

24. Szabo G, Petrasek J. Inflammasome activation and function in liver disease. Nature reviews Gastroenterology & hepatology. 2015;12(7):387-400. [DOI:10.1038/nrgastro.2015.94] [PMID]

25. Burdette D, Haskett A, Presser L, McRae S, Iqbal J, Waris G. Hepatitis C virus activates interleukin-1β via caspase-1-inflammasome complex. The Journal of general virology. 2012;93(2):235-246. [DOI:10.1099/vir.0.034033-0] [PMID] [PMCID]

26. Tehrani FH, Moradi M, Ghorbani N. Bacterial Etiology and Antibiotic Resistance Patterns in Neonatal Sepsis in Tehran during 2006-2014. Iranian Journal of Pathology. 2017;12(4):356. [Article] [PMCID] [PMID]

27. Niakan S, Niakan M, Hesaraki S, Nejadmoghaddam MR, Moradi M, Hanafiabdar M, et al. Comparison of the Antibacterial Effects of Nanosilver With 18 Antibiotics on Multidrug Resistance Clinical Isolates of Acinetobacter baumannii. Jundishapur J Microbiol. 2013;6(5):e8341. [Article] [DOI:10.5812/jjm.8341]