year 13, Issue 1 (March - April 2019)                   Iran J Med Microbiol 2019, 13(1): 80-88 | Back to browse issues page

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Heidari K, Kaboosi H, Jamali A, Ghaemi E A, Peyravii Ghadikolaii F. Prevalence of Pathogenic Genes cagA and vacA of Helicobacter pylori Isolated in Patients with Digestive Disorders. Iran J Med Microbiol 2019; 13 (1) :80-88
1- PhD Student, Department of Microbiology, Ayatollah Amoli branch, Islamic Azad University, Amol, Iran
2- Assistant Professor, Department of Microbiology, Ayatollah Amoli branch, Islamic Azad University, Amol, Iran ,
3- Assistant Professor, Department of Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
4- Professor, Department of Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
5- Assistant Professor. Department of Biology, Ghaemshahr branch, Islamic Azad University, Ghaemshahr, Iran
Abstract:   (6666 Views)
Background and Aims: Helicobacter pylori is the main cause of various gastroduodenal diseases. It is estimated that app roximately, more than half of the adult population in developed countries and 90% of people in developing countries infected with H. pylori. H. pylori infection may be related to Genetic of virulence factors and environmental factors. The aim of this study was to assess of frequency cagA and vacA genes of H. pylori isolated from patients with Gastrointestinal Disorders.
Materials and Methods: This cross-sectional descriptive study carried out on 120 patients with gastrointestinal diseases in Gorgan city in 2017. (40 patients of gastric cancer, 40 patients of peptic ulcer, 40 patients of without cancer and ulcer). After genomic DNA extraction PCR was carried out using specific H.pylori primers.
Results: Overall, 120 H.pylori strains were isolated. The frequency of cagA was %67.5 in gastric cancer, %60 in peptic ulcer and %45 in patiens without ulcer and gastric cancer. Also frequency of vacA gene was detected %55 in gastric cancer, %40 in peptic ulcer and %27.5 in patiens without ulcer and gastric cancer.
Conclusion: Based on our findings it seems that the cag A and vac A genes were virulence among H. pylori isolated from studied patients. The frequency of cagA and vacA genes H. pylori were than in gastric cancer and peptic ulcer patients.
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Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2018/11/24 | Accepted: 2019/06/18 | ePublished: 2019/10/2

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