year 11, Issue 1 (March - April 2017)                   Iran J Med Microbiol 2017, 11(1): 90-94 | Back to browse issues page

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Ashrafganjooyi S B, Saeid N, Alamian S, Khalili M. Survey of medicine susceptibility in Brucella melitensis isolated from raw milk of sheep and goat in Kerman. Iran J Med Microbiol. 2017; 11 (1) :90-94
1- Department Microbiology, Faculty of Afzalipour Medicine, Kerman, Iran ,
2- Department Microbiology, Faculty of Afzalipour Medicine, Kerman, Iran
3- Department of Brucella, Razi Vaccine and Serum Research Institute, Agricutaral Research education and Extension organization, Karaj, Iran
4- Department of pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Abstract:   (5981 Views)

Background and Aim: Brucellosis is one of the most common zoonotic disease between human and cattle breeding. Brucellosis is endemic in all regions of Iran. Since brucella is an arbitrary intracellular organism and a limited range of antibiotics are effective to it, this study is based on the survey of the antibiotic sensitivity to the 9 isolated B.melitensis from raw milk in tribes population.

Materials and Methods: Antimicrobial susceptibility of 9 B.melitensis bacteria isolated from nomadic livestock population’s raw milk were studied in the laboratory. The minimum inhibitory concentration (MIC) of tested antimicrobial substances were measured by E- test. To interpret the results, CLSI standards were used for bacteria growth.

Results and Conclusions: 9 isolates had a high sensitivity to Doxycycline, two isolates were resistant against three antibiotics Streptomycin (MIC≥240μg/mL) Cotrimoxazole (MIC≥256μg/mL) and Rifampin (MIC≥30μg/mL) and six other isolates were showed Intermediate and sensitivity to former antibiotics. According to results, resistance to Rifampin and Streptomycin and Cotrimoxazole were the important result of this article.

Full-Text [PDF 621 kb]   (1325 Downloads)    
Type of Study: Brief report | Subject: Medical Bacteriology
Received: 2016/09/27 | Accepted: 2017/01/21 | ePublished: 2017/03/16

1. Valdezate S, Navarro A, Medina-Pascual MJ, Garrasco G, Saez-Nieto JA. Molecular screening for Rifampicin and fluoroquinolone resistance in a clinical population of Brucella melitensis. J Antimicrob Chemother 2010; 65(1): 51-53. [PubMed]
2. Marianelli C, Ciuchini F, Tarantino M, Pasquali p, Adone R. Genetic Bases of the Rifampin Resistance Phenotype in Brucella SPP. J Clin Microbiol 2004; 42(12):5439-5443. [PubMed]
3. Kaya O, Akcam FZ, Yayli G. Investigation of the in vitro activities of various antibiotics against Brucella melitensis strains. Turk J Med Sci 2012; 42(1): 145-148. [Article]
4. Bailly& Scott’s, Forbes BA, Sahm DF, Weissfeld AS. Diagnostic Microbiology. 12nded. Mosby; 2007.
5. Bayram Y, Korkoca H, Aypak C, Parlak M, Cikman A, Kilic S, et al. Antimicrobial Susceptibilities of Brucella Isolates from various clinical speciements. International Journal of Medical Sciences. 2011; 8(3): 198-202. [PubMed]
6. Zowghi E, Ebadi A, Yarahmadi M. Isolation and identification of Brucella organisms in Iran. Iranian Journal of Clinical Infectious Disease. 2008; 3(4): 185-8. [in Persian] [Article]
7. Alikhani A, Honarmand H, Dehgani N, Hedarzadeh A. Microbiological survey and antibiotic sensitivity in patients with positive culture and positive serological wright test. Magazine of Mazandaran Medical Sciences University(JMUMS). 2008; 18(68): 74-8. [in Persian]
8. Peridogahe H, Aligoli M, Dehghan M, Mleknejad P. Determination of Antibiotic Sensitivity in Brucella melitensis Isolated from patient’s blood by MIC method. Research and scientific magazine Ardabil university. 1999; 8(1): 20-28. [in Persian]
9. Hashim R, Ahmad N, Zahidi JM, Tay BY, Mohd Noor A, Zainal S, et al. Indentification and In vitro Antimicrobial Susceptibility of Brucella species Isolated from Human Brucellosis. International Journal of Microbiology. 2014; ID 596245, 5 Pages.
10. Maves R, Castillo R, Gaillen A, Espinosa B, Meza R, Espinoza N, et al. Antimicrobial susceptibility of Brucella melitensis Isolates in Peru. Antimicrobial Agents and chemotherapy. 2011; 1279-81. [PubMed]

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