Survey of medicine susceptibility in Brucella melitensis isolated from raw milk of sheep and goat in Kerman

Ashrafganjooyi, Shahrnaz banu; Saeid, Nosrat; Alamian, Saeid; Khalili, Mohamad

year 11, Issue 1 (March - April 2017) Iran J Med Microbiol 2017, 11(1): 90-94 | Back to browse issues page

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Ashrafganjooyi S B, Saeid N, Alamian S, Khalili M. Survey of medicine susceptibility in Brucella melitensis isolated from raw milk of sheep and goat in Kerman. Iran J Med Microbiol 2017; 11 (1) :90-94
URL: http://ijmm.ir/article-1-625-en.html

Survey of medicine susceptibility in Brucella melitensis isolated from raw milk of sheep and goat in Kerman

Shahrnaz banu Ashrafganjooyi¹

, Nosrat Saeid²

, Saeid Alamian³

, Mohamad Khalili⁴

1- Department Microbiology, Faculty of Afzalipour Medicine, Kerman, Iran , sh_ashrafganjooyi@kmu.ac.ir
2- Department Microbiology, Faculty of Afzalipour Medicine, Kerman, Iran
3- Department of Brucella, Razi Vaccine and Serum Research Institute, Agricutaral Research education and Extension organization, Karaj, Iran
4- Department of pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract: (9075 Views)

Background and Aim: Brucellosis is one of the most common zoonotic disease between human and cattle breeding. Brucellosis is endemic in all regions of Iran. Since brucella is an arbitrary intracellular organism and a limited range of antibiotics are effective to it, this study is based on the survey of the antibiotic sensitivity to the 9 isolated B.melitensis from raw milk in tribes population.

Materials and Methods: Antimicrobial susceptibility of 9 B.melitensis bacteria isolated from nomadic livestock population’s raw milk were studied in the laboratory. The minimum inhibitory concentration (MIC) of tested antimicrobial substances were measured by E- test. To interpret the results, CLSI standards were used for bacteria growth.

Results and Conclusions: 9 isolates had a high sensitivity to Doxycycline, two isolates were resistant against three antibiotics Streptomycin (MIC≥240μg/mL) Cotrimoxazole (MIC≥256μg/mL) and Rifampin (MIC≥30μg/mL) and six other isolates were showed Intermediate and sensitivity to former antibiotics. According to results, resistance to Rifampin and Streptomycin and Cotrimoxazole were the important result of this article.

Keywords: Antibiotic sensitivity, Brucella melitensis, Raw milk, Kerman

Full-Text [PDF 621 kb] (2629 Downloads)

Type of Study: Brief Original Article | Subject: Medical Bacteriology
Received: 2016/09/27 | Accepted: 2017/01/21 | ePublished: 2017/03/16

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