Occurrence of Metallo-β-lactamase Variants Among Heterogeneous Population of Carbapenem Resistant Clinical

Anjum, Hasnain; Mitu, Shomaia Yasmin; Arefin, Md. Shamsul; Mitu, Meftahul Jannat; Hossain, Md. Sabir; Islam, Salequl; Rumi, M.A. Karim; Rahman, M. Hasibur

year 18, Issue 6 (November - December 2024) Iran J Med Microbiol 2024, 18(6): 352-363 | Back to browse issues page

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Anjum H, Mitu S Y, Arefin M S, Mitu M J, Hossain M S, Islam S, et al . Occurrence of Metallo-β-lactamase Variants Among Heterogeneous Population of Carbapenem Resistant Clinical from Savar, Bangladesh. Iran J Med Microbiol 2024; 18 (6) :352-363
URL: http://ijmm.ir/article-1-2462-en.html

Occurrence of Metallo-β-lactamase Variants Among Heterogeneous Population of Carbapenem Resistant Clinical from Savar, Bangladesh

Hasnain Anjum¹

, Shomaia Yasmin Mitu¹

, Md. Shamsul Arefin¹

, Meftahul Jannat Mitu¹

, Md. Sabir Hossain²

, Salequl Islam¹

, M.A. Karim Rumi³

, M. Hasibur Rahman⁴

1- Department of Microbiology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
2- Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
3- Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA
4- Department of Microbiology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh , hasiburku@juniv.edu

Abstract: (272 Views)

Background and Objectives: Worldwide dissemination of Metallo-β-Lactamases (MBLs) has been reported as a major mechanism of resistance to carbapenems in clinical Gram-negative isolates, including Pseudomonas aeruginosa (P. aeruginosa). The present study aimed to investigate the occurrence of MBL variants in carbapenem resistant P. aeruginosa isolates from Dhaka, Bangladesh.
Methods: Samples from patients (n=238) with urinary tract infections, wound infections, and catheter-associated infections from two academic hospitals of Savar, Dhaka were investigated. Carbapenem susceptibility of the isolates was tested against imipenem and meropenem. MBL-mediated carbapenem resistance was investigated phenotypically and through PCR amplification of four MBL variants. Plasmid extraction was conducted to observe association with MBL carriage. ERIC-PCR was carried out to determine possible epidemiological linkages.
Results: The presence of P. aeruginosa was detected in 22% (53/238) of the collected clinical samples. Resistance to carbapenems was present among 30% (16/53) of these clinical isolates, which is more than 2-fold higher compared to that of previous studies. Phenotypic expression of MBL was detected in 90% (14/16) of resistant isolates. Molecular analysis revealed that 63% (10/16) of the carbapenem-resistant isolates carried at least one of the MBL variants, either bla-VIM or bla-NDM-1. All positive bla-NDM-1 variants carried a 0.5 MDa plasmid. ERIC-PCR revealed highly heterogeneous nature of P. aeruginosa isolates indicating multiple sources of infection within the hospital.
Conclusion: These findings indicate an increased MBL-mediated resistance to carbapenem in P. aeruginosa, which poses a serious threat to the treatment of infections among hospitalized patients.

Keywords: Carbapenem-resistance, Genetic Variants, Metallo-β-lactamase, Nosocomial Infection, Pseudomonas aeruginosa

Full-Text [PDF 772 kb] (70 Downloads)

Type of Study: Original Research Article | Subject: Antibiotic Resistance
Received: 2024/08/1 | Accepted: 2024/12/31 | ePublished: 2025/01/29

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