year 18, Issue 2 (March - April 2024)                   Iran J Med Microbiol 2024, 18(2): 80-88 | Back to browse issues page

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Hamayeli H, Abdi Ali A, Attaran B, Shafiei M, modaresi M. Isolation, Identification and Antibiotic Resistance of Anaerobic Bacteria in Sputum of Cystic Fibrosis Patients from children's medical center of Tehran, Iran. Iran J Med Microbiol 2024; 18 (2) :80-88
1- Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
2- Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran ,
3- Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
4- Growth and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran & Pediatric Pulmonary Disease and Sleep Medicine Research Center, Pediatric Centre of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (267 Views)

Background: Following genetic defects in cystic fibrosis (CF), mucus accumulation and hypoxic gradients develop in the lungs creating conditions for anaerobic bacterial colonization. This study investigated the difference between the prevalence of some anaerobic bacteria and antibiotic resistance in healthy and CF groups.
Materials and Methods: In this research, anaerobic bacteria were investigated in sputum samples of CF patients (50 people) and healthy individuals (18 people) in Tehran, Iran by culture method during 2021-2022. The sputum samples were cultured on Brucella blood agar, Brain heart infusion agar with vancomycin, Laked brucella blood agar with kanamycin and vancomycin, and Brucella blood agar with neomycin and vancomycin. Biochemical methods identified Gram-negative isolates and Gram-positive isolates were identified using 16S rRNA sequencing. In addition, the MIC of the bacterial isolates was tested for ampicillin, clindamycin, and ampicillin/sulbactam using E-test strips.
Results: In the culture-based method, anaerobic bacteria were isolated in 33% and 24% of the healthy and CF groups, respectively. Bacterial isolates detected include Dialister spp., Fusobacterium spp., Lactobacillus spp., and Prevotella spp. The Fusobacterium isolates from the healthy group were sensitive to ampicillin, and 33% of Fusobacterium isolates from the CF group showed resistance to this antibiotic.
Conclusions: In this study, the presence of anaerobic bacteria in the lungs of healthy and CF individuals was determined. Although the total frequency of these bacteria in the healthy group is higher than the CF group. The abundance of Gram-positive Lactobacillus bacteria, as potentially beneficial bacteria, is lower in CF patients than in healthy individuals.

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Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2024/02/9 | Accepted: 2024/05/8 | ePublished: 2024/05/25

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