year 18, Issue 2 (March - April 2024)                   Iran J Med Microbiol 2024, 18(2): 6-6 | Back to browse issues page

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Amirmozafari N, Haddadi A, Mirnejad R, Angaji S A, Babapour E. Relationship Between Drug Efflux Pumps and Resistance to Ciprofloxacin in Clinical Isolates of Acinetobacter baumannii. Iran J Med Microbiol 2024; 18 (2) :6-6
1- Microbiology Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2- Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
3- Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
4- Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
5- Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran ,
Abstract:   (207 Views)

Background and Aim: Acinetobacter baumannii (A. baumannii) is an important bacterium that can cause multidrug-resistant nosocomial infections in patients who are admitted to different hospital wards. Various factors play role in resistance of this bacterium to antibiotics, one of the most important of which is the presence of drug efflux pumps. This study aimed to investigate the presence of the AdeABC efflux system and its role in drug resistance by inactivating them.
Materials & Methods: For this purpose, clinical samples were collected from three hospitals in Tehran, Iran for one year. By culture and biochemical methods, the initial diagnosis was made for the identification of A. baumannii. At last, through a molecular method, the identified prototypes were confirmed. To investigate the role of antibiotic efflux pump in drug resistance, minimum inhibitory concentration (MIC) for ciprofloxacin in the presence and absence of an efflux pump inhibitor, carbonyl cyanide 3-chlorophenylhy­drazone (CCCP), was determined by microdilution method. Additionally, the presence of adeB, adeR, and adeS genes related to the AdeABC depletion system was investigated by PCR.
Results: The results showed that more than 98% of the isolated bacteria had adeB, adeR, and adeS genes in the AdeABC depletion system. Approximately 47.18% of these bacteria displayed a fourfold reduction in ciprofloxacin MIC levels in the presence of CCCP.
Conclusion:  Administration of a suitable antibiotic along with a safe efflux pump inhibitor for the treatment of A. baumannii infections can help to reduce material and spiritual damages caused by resistant bacteria

Type of Study: Original Research Article | Subject: Antibiotic Resistance
Received: 2024/02/14 | Accepted: 2024/05/15 | ePublished: 2024/05/25

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