year 17, Issue 2 (March - April 2023)                   Iran J Med Microbiol 2023, 17(2): 186-193 | Back to browse issues page


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Bahmanpoor N, Hamdi S M M, Ataee R. Real- Time - RT PCR Study of Extract of Ammi visnaga on Pseudomonas aeruginosa Exo A and Exo S Genes Expression. Iran J Med Microbiol 2023; 17 (2) :186-193
URL: http://ijmm.ir/article-1-1435-en.html
1- Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Microbiology, School of Medicine and Applied Microbiology Research Center, Institute of Toxicology and Systems Biology, Baqiyatullah University of Medical Sciences, Tehran, Iran
3- Department of Microbiology, School of Medicine and Clinical Research Development Committee, Baqiyatullah University of Medical Sciences, Tehran, Iran , ataee216@gmail.com
Abstract:   (1666 Views)

Background and Aim: Pseudomonas aeruginosa exotoxins are responsible for pathogenicity and antibiotic resistant. The aim of this study was to investigate the effects of the Ammi. visnaga (A. visnaga) extract on the P. aeruginosa exotoxin A and S genes expression.
Materials and Methods: Antibacterial effects of the A. visnaga ethanolic extract were performed using the micro dilution technique in sequential dilutions. Then, the Real-Time- RT PCR method was used to analyze the level of expression of the exotoxin A and S genes of the bacterium. Real-Time PCR data were analyzed using Graph Pad Software Inc. San Diego (GRAPHPAD PRISM 6CA, USA).
Results: Results showed that, the expression of Exotoxin A and S genes was significantly (P <0.001) reduced in comparing the expression level of the reference gene and control. It had a significant effect on the inhibition of bacterial growth at different concentrations but the lowest inhibitory concentration was observed at 3.125 µg/well.
Conclusion: Accordingly, the extract of A. visnaga can decrease the expression of the Exotoxin A and S genes of P. aeruginosa and consequently it may decrease the pathogenicity of bacteria. These phenomena (exoA and exoS expression inhibition) may involve reducing the antibiotic resistance of P. aeruginosa.

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Type of Study: Original Research Article | Subject: Antimicrobial Substances
Received: 2022/05/28 | Accepted: 2023/02/2 | ePublished: 2023/03/30

References
1. Dehghan D, Ramandi MF, Taheri RA. Investigation of Synergism of Silver Nanoparticle and Erythromycin Inhibition and Detection of Exotoxin-A Gene in Pseudomonas aeruginosa Isolated from Burn Wounds Secretion. Iran J Med Microbiol. 2020;14(4):379-89. [DOI:10.30699/ijmm.14.4.379]
2. Rezaei F, Saderi H, Boroumandi S, Faghihzadeh S. Relation between Resistance to Antipseudomonal β-Lactams and ampC and mexC Genes of Pseudomonas aeruginosa. Iran J Pathol. 2016;11(1):47-53.
3. Khashei R, Navabi Z, Mohebi S, Samadi N. Antibiotic Resistance Among Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Obtained From Shiraz Nemazi Hospital ICU Wards. Iran J Med Microbiol. 2018;12(4):294-300. [DOI:10.30699/ijmm.12.4.294]
4. Liaqat I, Liaqat M, Tahir HM, Ali NM, Arshad M, Arshad N. Motility effects biofilm formation in Pseudomonas aeruginosa and Enterobacter cloacae. Pak J Pharm Sci. 2019;32(3):927-32.
5. Candido Caçador N, Paulino da Costa Capizzani C, Gomes Monteiro Marin Torres LA, Galetti R, Ciofu O, da Costa Darini AL, et al. Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients. PLoS One. 2018;13(11):e0208013. [DOI:10.1371/journal.pone.0208013] [PMID] [PMCID]
6. Mousahasankhani H, Kariminik A. Evaluation of the Expression of NLRP1 Inflammasome in Patients with Bacterial Septicemia. Iran J Med Microbiol. 2020;14(3):201-12. [DOI:10.30699/ijmm.14.3.201]
7. Zaidenstein R, Miller A, Tal-Jasper R, Ofer-Friedman H, Sklarz M, Katz DE, et al. Therapeutic Management of Pseudomonas aeruginosa Bloodstream Infection Non-Susceptible to Carbapenems but Susceptible to "Old" Cephalosporins and/or to Penicillins. Microorganisms. 2018;6(1):9. [DOI:10.3390/microorganisms6010009] [PMID] [PMCID]
8. Meskini M, Esmaeili D. The study of formulated Zoush ointment against wound infection and gene expression of virulence factors Pseudomonas aeruginosa. BMC Complement Altern Med. 2018;18(1):185. [DOI:10.1186/s12906-018-2251-4] [PMID] [PMCID]
9. Ertugrul BM, Oryasin E, Lipsky BA, Willke A, Bozdogan B. Virulence genes fliC, toxA and phzS are common among Pseudomonas aeruginosa isolates from diabetic foot infections. Infect Dis. 2018;50(4):273-9. [DOI:10.1080/23744235.2017.1393839] [PMID]
10. Khodayary R, Nikokar I, Mobayen M, Afrasiabi F, Araghian A, Elmi A, et al. High incidence of type III secretion system associated virulence factors (exoenzymes) in Pseudomonas aeruginosa isolated from Iranian burn patients. BMC Res Notes. 2019;12(1):1-6. [DOI:10.1186/s13104-019-4071-0] [PMID] [PMCID]
11. Khalil N, Bishr M, Desouky S, Salama O. Ammi visnaga L., a potential medicinal plant: A review. Molecules. 2020;25(2):301. [DOI:10.3390/molecules25020301] [PMID] [PMCID]
12. Grădinaru AC, Trifan A, Şpac A, Brebu M, Miron A, Aprotosoaie AC. Antibacterial activity of traditional spices against lower respiratory tract pathogens: combinatorial effects of Trachyspermum ammi essential oil with conventional antibiotics. Lett Appl Microbiol. 2018;67(5):449-57. [DOI:10.1111/lam.13069] [PMID]
13. Rath S, Padhy RN. Monitoring in vitro antibacterial efficacy of 26 Indian spices against multidrug resistant urinary tract infecting bacteria. Integr Med Res. 2014;3(3):133-41. [DOI:10.1016/j.imr.2014.04.002] [PMID] [PMCID]
14. Moein MR, Zomorodian K, Pakshir K, Yavari F, Motamedi M, Zarshenas MM. Trachyspermum ammi (L.) sprague: chemical composition of essential oil and antimicrobial activities of respective fractions. J Evid Based Complementary Altern Med. 2015;20(1):50-6. [DOI:10.1177/2156587214553302] [PMID]
15. CLSI Subcommittee on Antimicrobial Susceptibility Testing M100 tE. Newsletter; 2022.
16. Ataee RA, Mehrabi Tavana A, Hosseini SMJ, Moridi K, Ghorban ali Zadegan M. A Method for Antibiotic Suceptility Testing: Applicable and Acuurate. Jundishapur J Microbiol. 2012;5(1):343-5.
17. Elmouaden C, Laglaoui A, Ennanei L, Bakkali M, Abid M. Virulence genes and antibiotic resistance of Pseudomonas aeruginosa isolated from patients in the Northwestern of Morocco. J Infect Dev Ctries. 2019;13(10):892-8. [DOI:10.3855/jidc.10675] [PMID]
18. Hoseinpoor Mohammad Abadi Z, Ebrahimi Kahrizsangi A, Mokhtari A. Evaluation of the Effect of a Number of Commercial Disinfectants on Pseudomonas aeruginosa Isolates Obtained from Human Infection Cases. Iran J Med Microbiol. 2020;14(2):138-53. [DOI:10.30699/ijmm.14.2.138]
19. Mokhtari K, Amini K. Identification of virulence genes in Pseudomonas aeruginosa isolated from human and animal samples by multiplex-PCR and their antibiotic resistance pattern. Iran J Med Microbiol. 2019;13(4):294-301. [DOI:10.30699/ijmm.13.4.294]
20. Pang Z, Raudonis R, Glick BR, Lin T-J, Cheng Z. Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies. Biotechnol Adv. 2019;37(1):177-92. [DOI:10.1016/j.biotechadv.2018.11.013] [PMID]
21. Lee EJ, Cowell BA, Evans DJ, Fleiszig SMJ. Contribution of ExsA-regulated factors to corneal infection by cytotoxic and invasive Pseudomonas aeruginosa in a murine scarification model. Investig Ophthalmol Vis Sci. 2003;44(9):3892-8. [DOI:10.1167/iovs.02-1302] [PMID]
22. Verove J, Bernarde C, Bohn Y-ST, Boulay F, Rabiet M-J, Attree I, et al. Injection of Pseudomonas aeruginosa Exo toxins into host cells can be modulated by host factors at the level of translocon assembly and/or activity. PloS one. 2012;7(1):e30488. [DOI:10.1371/journal.pone.0030488] [PMID] [PMCID]
23. Krall R, Zhang Y, Barbieri JT. Intracellular membrane localization of Pseudomonas ExoS and Yersinia YopE in mammalian cells. J Biol Chem. 2004;279(4):2747-53. [DOI:10.1074/jbc.M301963200] [PMID]
24. Khan R, Zakir M, Afaq SH, Latif A, Khan AU. Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains. J Infect Dev Ctries. 2010;4(05):292-300. [DOI:10.3855/jidc.621] [PMID]
25. Koriem KMM, Arbid MS, El-Attar MA. Acute and subacute toxicity of on rats. Interdiscip Toxicol. 2019;12(1):26-35. [DOI:10.2478/intox-2019-0004] [PMID] [PMCID]
26. Aydoğmuş-Öztürk F, Jahan H, Beyazit N, Günaydın K, Choudhary MI. The anticancer activity of visnagin, isolated from Ammi visnaga L., against the human malignant melanoma cell lines, HT 144. Mol Biol Rep. 2019;46(2):1709-14. [DOI:10.1007/s11033-019-04620-1] [PMID]
27. Ghods Zadeh M, Moradi Bidhendi S, Ashrafi F. Comparative Study of Garlic Extract Effect on the Expression of Genes Involved in Quorum Sensing in Pseudomonas aeruginosa and the Antibiotic Tobramycin. Iran J Med Microbiol. 2021;15(1):107-20. [DOI:10.30699/ijmm.15.1.107]
28. Singh A, Ahmad A. Antioxidant activity of essential oil extracted by SC-CO2 from seeds of Trachyspermum ammi. Medicines. 2017;4(3):53. [DOI:10.3390/medicines4030053] [PMID] [PMCID]
29. Travaini ML, Sosa GM, Ceccarelli EA, Walter H, Cantrell CL, Carrillo NJ, et al. Khellin and Visnagin, Furanochromones from Ammi visnaga (L.) Lam., as Potential Bioherbicides. J Agric Food Chem. 2016;64(50):9475-87. [DOI:10.1021/acs.jafc.6b02462] [PMID]
30. Goswami N, Chatterjee S. Assessment of free radical scavenging potential and oxidative DNA damage preventive activity of Trachyspermum ammi L.(carom) and Foeniculum vulgare Mill. (fennel) seed extracts. Biomed Res Int. 2014;2014:582767. [DOI:10.1155/2014/582767] [PMID] [PMCID]
31. Gellatly SL, Hancock REW. Pseudomonas aeruginosa : new insights into pathogenesis and host defenses. Pathog Dis. 2013;67(3):159-73. [DOI:10.1111/2049-632X.12033] [PMID]
32. Blair JMA, Webber MA, Baylay AJ, Ogbolu DO, Piddock LJV. Molecular mechanisms of antibiotic resistance. Nat Rev Microbiol. 2015;13(1):42-51. [DOI:10.1038/nrmicro3380] [PMID]

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