Real- Time - RT PCR Study of Extract of Ammi visnaga on Pseudomonas aeruginosa Exo A and Exo S Genes Expression

Bahmanpoor, Negin; Hamdi, Seyed Mohammad Mahdi; Ataee, RamezanAli

doi:10.30699/ijmm.17.2.186

year 17, Issue 2 (March - April 2023) Iran J Med Microbiol 2023, 17(2): 186-193 | Back to browse issues page

‎ 10.30699/ijmm.17.2.186

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Bahmanpoor N, Hamdi S M M, Ataee R. Real- Time - RT PCR Study of Extract of Ammi visnaga on Pseudomonas aeruginosa Exo A and Exo S Genes Expression. Iran J Med Microbiol 2023; 17 (2) :186-193
URL: http://ijmm.ir/article-1-1435-en.html

Real- Time - RT PCR Study of Extract of Ammi visnaga on Pseudomonas aeruginosa Exo A and Exo S Genes Expression

Negin Bahmanpoor¹

, Seyed Mohammad Mahdi Hamdi²

, RamezanAli Ataee

1- Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Microbiology, School of Medicine and Applied Microbiology Research Center, Institute of Toxicology and Systems Biology, Baqiyatullah University of Medical Sciences, Tehran, Iran
3- Department of Microbiology, School of Medicine and Clinical Research Development Committee, Baqiyatullah University of Medical Sciences, Tehran, Iran , ataee216@gmail.com

Abstract: (1563 Views)

Background and Aim: Pseudomonas aeruginosa exotoxins are responsible for pathogenicity and antibiotic resistant. The aim of this study was to investigate the effects of the Ammi. visnaga (A. visnaga) extract on the P. aeruginosa exotoxin A and S genes expression.
Materials and Methods: Antibacterial effects of the A. visnaga ethanolic extract were performed using the micro dilution technique in sequential dilutions. Then, the Real-Time- RT PCR method was used to analyze the level of expression of the exotoxin A and S genes of the bacterium. Real-Time PCR data were analyzed using Graph Pad Software Inc. San Diego (GRAPHPAD PRISM 6CA, USA).
Results: Results showed that, the expression of Exotoxin A and S genes was significantly (P <0.001) reduced in comparing the expression level of the reference gene and control. It had a significant effect on the inhibition of bacterial growth at different concentrations but the lowest inhibitory concentration was observed at 3.125 µg/well.
Conclusion: Accordingly, the extract of A. visnaga can decrease the expression of the Exotoxin A and S genes of P. aeruginosa and consequently it may decrease the pathogenicity of bacteria. These phenomena (exoA and exoS expression inhibition) may involve reducing the antibiotic resistance of P. aeruginosa.

Keywords: Antibacterial, Gene Expression, Ammi. visnaga, Exotoxins, Pseudomonas aeruginosa

Full-Text [PDF 749 kb] (351 Downloads) | | Full-Text (HTML) (348 Views)

Type of Study: Original Research Article | Subject: Antimicrobial Substances
Received: 2022/05/28 | Accepted: 2023/02/2 | ePublished: 2023/03/30

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