year 15, Issue 5 (September - October 2021)                   Iran J Med Microbiol 2021, 15(5): 552-568 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Moussavi amin P, Tabatabaee Bafroee A S, Khalili Hadad B. Evaluating the Effect of Supernatant Collected from the Culture of Bifidobacterium bifidum on the Increase of Angiogenesis in Acute Wound Healing. Iran J Med Microbiol 2021; 15 (5) :552-568
1- Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Roudehen Branch, Tehran, Iran ,
Abstract:   (2019 Views)

Background and Objective: Wound healing is the result of interactions between cytokines, growth factors, blood, and extracellular matrix. Facing this challenging issue has become one of the essential concerns in health and medical fields, needing different remedies. One of the newest treatments in wound healing is the application of probiotic bacteria. Therefore, the aim of the current study is to evaluate the effect of Bifidobacterium bifidum probiotic bacteria on acute wound healing and exploring the potential of their supernatant on increasing angiogenesis in female mice in the form of biological dressing.
Materials and Methods: 44 female BALB/c mice were studied into six groups in two phases of 7 and 14 days. After the wounding process, wound sizes were measured by a digital caliper every 48 hours. Mice were dressed and treated. Histological samples were studied, and the results were analyzed statistically.
Results: Bifidobacterium bifidum probiotic bacteria not only show exciting potential as a therapeutic and effective agent but also our examination proved that application of this probiotic plus Aloe vera hydrogel (experimental group 1) can significantly reduce the wound healing duration and increases angiogenesis (P<0.05).
Conclusion: According to the findings of this study, the designed biological dressing, including Bifidobacterium bifidum supernatant and Aloe vera hydrogel, can be used in acute wound healing after further studies.

Full-Text [PDF 1622 kb]   (582 Downloads) |   |   Full-Text (HTML)  (454 Views)  
Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2021/03/13 | Accepted: 2021/08/13 | ePublished: 2021/09/5

1. Yousef H, Alhajj M, Sharma S. Anatomy, skin (integument), epidermis. 2017.
2. Jimi S, Jaguparov A, Nurkesh A, Sultankulov B, Saparov A. Sequential delivery of cryogel released growth factors and cytokines accelerates wound healing and improves tissue regeneration. Frontiers in Bioengineering and Biotechnology. 2020;8:345 [DOI:10.3389/fbioe.2020.00345] [PMID] [PMCID]
3. Markowiak P, Śliżewska K. Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients. 2017;9(9):1021. [DOI:10.3390/nu9091021] [PMID] [PMCID]
4. Zommiti M, Feuilloley MG, Connil N. Update of probiotics in human world: a nonstop source of benefactions till the end of time. Microorganisms. 2020;8(12):1907. [DOI:10.3390/microorganisms8121907] [PMID] [PMCID]
5. Plaza-Diaz J, Ruiz-Ojeda FJ, Gil-Campos M, Gil A. Mechanisms of action of probiotics. Advances in Nutrition. 2019;10(suppl_1):S49-S66. [DOI:10.1093/advances/nmy063] [PMID] [PMCID]
6. Satish L, Gallo PH, Johnson S, Yates CC, Kathju S. Local probiotic therapy with Lactobacillus plantarum mitigates scar formation in rabbits after burn injury and infection. Surg infections. 2017;18(2):119-27. [DOI:10.1089/sur.2016.090] [PMID] [PMCID]
7. Lolou V, Panayiotidis MI. Functional role of probiotics and prebiotics on skin health and disease. Ferment Technol. 2019;5(2):41. [DOI:10.3390/fermentation5020041]
8. Mohammedsaeed W. Characterisation of the potential of probiotics or their extracts as therapy for skin: The University of Manchester (United Kingdom); 2015.
9. Mirani B, Pagan E, Currie B, Siddiqui MA, Hosseinzadeh R, Mostafalu P, et al. An advanced multifunctional hydrogel‐based dressing for wound monitoring and drug delivery. Adv. Healthc. 2017;6(19):1700718. [DOI:10.1002/adhm.201700718] [PMID] [PMCID]
10. Saberian M, Delshad ES, Habibi M. The Effect of Bifidobacterium Bifidum Supernatant and Cell Mass on the Proliferation Potential of Rat Bone Marrow-Derived Stromal Cells. IJMS. 2020;45(4):269.
11. Masson‐Meyers DS, Andrade TA, Caetano GF, Guimaraes FR, Leite MN, Leite SN, et al. Experimental models and methods for cutaneous wound healing assessment. Int j Exp Pathol. 2020;101(1-2):21-37. [DOI:10.1111/iep.12346] [PMID] [PMCID]
12. Yasuda K, Ogushi M, Nakashima A, Nakano Y, Suzuki K. Accelerated wound healing on the skin using a film dressing with β-glucan paramylon. In Vivo. 2018;32(4):799-805. [DOI:10.21873/invivo.11310] [PMID] [PMCID]
13. Hesari AK, Shahrooz R, Ahmadi A, Malekinejad H, Saboory E. Crocin prevention of anemia-induced changes in structural and functional parameters of mice testes. J. Appl. Biomed. 2015;13(3):213-23. [DOI:10.1016/j.jab.2015.02.001]
14. Shahrooz R, Agh N, Jafari N, Kalantari A, Jalili R, Karimi A. Effects of fish oil replacement with vegetable oils in rainbow trout (Oncorhynchus mykiss) fingerlings diet on growth performance and foregut histology. Turkish J. Fish. Aquat Sci. 2018;18(6):825-32. [DOI:10.4194/1303-2712-v18_6_09]
15. Gurina TS, Simms L. Histology, Staining. 2020.
16. Farghali H, Canová NK, Zakhari S. Hepatoprotective properties of extensively studied medicinal plant active constituents: possible common mechanisms. Pharm Biol. 2015;53(6):781-91. [DOI:10.3109/13880209.2014.950387] [PMID]
17. Brandi J, Cheri S, Manfredi M, Di Carlo C, Vanella VV, Federici F, et al. Exploring the wound healing, anti-inflammatory, anti-pathogenic and proteomic effects of lactic acid bacteria on keratinocytes. Scientific reports. 2020;10(1):1-14. [DOI:10.1038/s41598-020-68483-4] [PMID] [PMCID]
18. DiPietro LA. Angiogenesis and wound repair: when enough is enough. J. Leukoc Biol. 2016;100(5):979-84. [DOI:10.1189/jlb.4MR0316-102R] [PMID] [PMCID]
19. Tonnesen MG, Feng X, Clark RA, editors. Angiogenesis in wound healing. JIDSP; 2000: Elsevier. [DOI:10.1046/j.1087-0024.2000.00014.x] [PMID]
20. Lai Y, Di Nardo A, Nakatsuji T, Leichtle A, Yang Y, Cogen AL, et al. Commensal bacteria regulate Toll-like receptor 3-dependent inflammation after skin injury. Nat Med. 2009;15(12):1377-82. [DOI:10.1038/nm.2062] [PMID] [PMCID]
21. Veith AP, Henderson K, Spencer A, Sligar AD, Baker AB. Therapeutic strategies for enhancing angiogenesis in wound healing. Adv Drug Deliv Rev. 2019;146:97-125. [DOI:10.1016/j.addr.2018.09.010] [PMID] [PMCID]
22. Honnegowda TM, Kumar P, Udupa EGP, Kumar S, Kumar U, Rao P. Role of angiogenesis and angiogenic factors in acute and chronic wound healing. Aesthetic Plast Surg. 2015;2:243-9. [DOI:10.4103/2347-9264.165438]
23. Teplicki E, Ma Q, Castillo DE, Zarei M, Hustad AP, Chen J, et al. The Effects of Aloe vera on Wound Healing in Cell Proliferation, Migration, and Viability. Wounds: a compendium of clinical research and practice. 2018;30(9):263-8.
24. Sánchez M, González-Burgos E, Iglesias I, Gómez-Serranillos MP. Pharmacological update properties of Aloe vera and its major active constituents. Molecules. 2020;25(6):1324. [DOI:10.3390/molecules25061324] [PMID] [PMCID]
25. Darzi S, Paul K, Leitan S, Werkmeister JA, Mukherjee S. Immunobiology and Application of Aloe vera-Based Scaffolds in Tissue Engineering. Int. J. Mol. 2021;22(4):1708. [DOI:10.3390/ijms22041708] [PMID] [PMCID]
26. Johnson KE, Wilgus TA. Vascular endothelial growth factor and angiogenesis in the regulation of cutaneous wound repair. Advs wound care. 2014;3(10):647-61. [DOI:10.1089/wound.2013.0517] [PMID] [PMCID]
27. Lukic J, Chen V, Strahinic I, Begovic J, Lev‐Tov H, Davis SC, et al. Probiotics or pro‐healers: the role of beneficial bacteria in tissue repair. Wound Repair and Regen. 2017;25(6):912-22. [DOI:10.1111/wrr.12607] [PMID] [PMCID]
28. Halper J, Leshin L, Lewis S, Li W. Wound healing and angiogenic properties of supernatants from Lactobacillus cultures. Exp. Biol Med. 2003;228(11):1329-37. [DOI:10.1177/153537020322801111] [PMID]
29. Sinha A, Sagar S, Madhumathy M, Osborne WJ. Probiotic Bacteria in Wound Healing; An In-Vivo Study. Iranian Journal of Biotechnology. 2019;17(4):e2188.
30. Nagle SM, Waheed A, Wilbraham SC. Wound Assessment. StatPearls [Internet]. 2020.
31. Kuru B. The adventure of axillary treatment in early stage breast cancer. Eur. J. Breast Health. 2020;16(1):1. [DOI:10.5152/ejbh.2019.5157] [PMID] [PMCID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc