Evaluating the Effect of Supernatant Collected from the Culture of Bifidobacterium bifidum on the Increase of Angiogenesis in Acute Wound Healing

Moussavi amin, Pegah; Tabatabaee Bafroee, Akram Sadat; Khalili Hadad, Babak

doi:10.30699/ijmm.15.5.552

year 15, Issue 5 (September - October 2021) Iran J Med Microbiol 2021, 15(5): 552-568 | Back to browse issues page

‎ 10.30699/ijmm.15.5.552

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Moussavi amin P, Tabatabaee Bafroee A S, Khalili Hadad B. Evaluating the Effect of Supernatant Collected from the Culture of Bifidobacterium bifidum on the Increase of Angiogenesis in Acute Wound Healing. Iran J Med Microbiol 2021; 15 (5) :552-568
URL: http://ijmm.ir/article-1-1300-en.html

Evaluating the Effect of Supernatant Collected from the Culture of Bifidobacterium bifidum on the Increase of Angiogenesis in Acute Wound Healing

Pegah Moussavi amin¹

, Akram Sadat Tabatabaee Bafroee¹

, Babak Khalili Hadad

1- Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Roudehen Branch, Tehran, Iran , khalili@riau.ac.ir

Abstract: (4696 Views)

Background and Objective: Wound healing is the result of interactions between cytokines, growth factors, blood, and extracellular matrix. Facing this challenging issue has become one of the essential concerns in health and medical fields, needing different remedies. One of the newest treatments in wound healing is the application of probiotic bacteria. Therefore, the aim of the current study is to evaluate the effect of Bifidobacterium bifidum probiotic bacteria on acute wound healing and exploring the potential of their supernatant on increasing angiogenesis in female mice in the form of biological dressing.
Materials and Methods: 44 female BALB/c mice were studied into six groups in two phases of 7 and 14 days. After the wounding process, wound sizes were measured by a digital caliper every 48 hours. Mice were dressed and treated. Histological samples were studied, and the results were analyzed statistically.
Results: Bifidobacterium bifidum probiotic bacteria not only show exciting potential as a therapeutic and effective agent but also our examination proved that application of this probiotic plus Aloe vera hydrogel (experimental group 1) can significantly reduce the wound healing duration and increases angiogenesis (P<0.05).
Conclusion: According to the findings of this study, the designed biological dressing, including Bifidobacterium bifidum supernatant and Aloe vera hydrogel, can be used in acute wound healing after further studies.

Keywords: Probiotic, Bifidobacterium bifidum, Acute wound healing, Culture supernatant

Full-Text [PDF 1622 kb] (1256 Downloads) | | Full-Text (HTML) (1197 Views)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2021/03/13 | Accepted: 2021/08/13 | ePublished: 2021/09/5

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