year 15, Issue 3 (May - Jun 2021)                   Iran J Med Microbiol 2021, 15(3): 345-351 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mirzaei S, Keshavarzi F, Karami P. The Prevalence of H. Pylori cagA Gene in Patients with Gastric Ulcer. Iran J Med Microbiol. 2021; 15 (3) :345-351
URL: http://ijmm.ir/article-1-1184-en.html
1- Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2- Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran , f.keshavarzi@iausdj.ac.ir
3- Department of Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Abstract:   (1366 Views)

Background and Objective: Helicobacter Pylori (H. pylori) is one of the reasons for the gastric inflammation and peptic ulcers. It is a predisposing factor of gastric adenocarcinoma. Cytotoxin A encoded by the cagA gene is one of the major virulence factors in bacterial pathogenicity, which is of special importance due to genetic diversity in different geographical areas. The purpose of this study was to evaluate the prevalence of the cagA gene in the patients suffering from gastric ulcers.
Materials and Methods: Seventy-five paraffin blocks of stomach biopsy samples from patients infected with H. pylori and suffering from gastric ulcers were randomly evaluated in the province of Kermanshah in the spring of 2016, Iran. DNA samples extracted by the boiling method were investigated using PCR by the use of particular primer pairs for a protected area in the glmM gene, and the existence of the cagA gene.
Results: From the 75 samples, 56 cases (%74.66) were glmM-positive and among them, 39 cases (%69.64) tested positive for cagA gene. Comparing patients in terms of gender, the frequency of the cagA gene was 22 (%39.28) and 17 (%30.35) in males and females, respectively. Finally, comparing patients in terms of age, 16 cases (%21.33) were younger than 40 years old and 23 cases (%41.07) were older than 40 years old.
Conclusion: The results of this study showed significant association between the frequency of the cagA gene and peptic ulcer in the studied patients.
 

Full-Text [PDF 724 kb]   (421 Downloads) |   |   Full-Text (HTML)  (494 Views)  
Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2020/07/14 | Accepted: 2021/05/3 | ePublished: 2021/06/28

References
1. Yu C, Li L, Chen W, Jiao Y, Yang N, Yang E, Zhang J, Chen L, Li Y. Levofloxacin susceptibility testing for Helicobacter pylori in China: comparison of E-test and disk diffusion method. Helicobacter. 2011; 16:119-23. [DOI:10.1111/j.1523-5378.2011.00820.x] [PMID]
2. Ayala G, Escobedo-Hinojosa WI, de la Cruz-Herre- ra CF, Romero I. Exploring alternative treatments for Helicobacter pylori infection. World J Gastroenterol 2014; 20:1450-69. [DOI:10.3748/wjg.v20.i6.1450] [PMID] [PMCID]
3. Yamaoka Y, Kato M, Asaka M .2008. Geographic differences in gastric cancer incidence can be explained by differences between Helicobacter pylori strains. Intern Med 47: 1077-83. [DOI:10.2169/internalmedicine.47.0975] [PMID] [PMCID]
4. Polk DB, Peek Jr RM. Helicobacter pylori: gastric cancer and beyond. Nat Rev Cancer 2010; 10:403-14. [DOI:10.1038/nrc2857] [PMID] [PMCID]
5. Mendoza JA, Weinberger KK, Swan MJ. The Hsp60 protein of Helicobacter pylori displays chaperone ac- activity under acidic conditions. Biochem Biophys Rep2017; 9:95-9. [DOI:10.1016/j.bbrep.2016.11.011] [PMID] [PMCID]
6. Prasertpetmanee S, Mahachai V, Vilaichone RK. Improved efficacy of proton pump inhibitor - amoxicillin - clarithromycin triple therapy for Helicobacter pylori eradication in low clarithromycin resistance areas or tailored therapy. Helicobacter. 2013; 18:270-3. [DOI:10.1111/hel.12041] [PMID]
7. Atherton JC, EM Omar-El, JL Hale, RH A. In Differences. at motif phosphorylation tyrosine CagA pylori H on influences and, strains Asian East and western between. Microbiol Med J 2008; 7(10): 57 - 62.
8. Thung I, Aramin H, Vavinskaya V, Gupta S, Park JY, Crowe SE, Valasek MA. Review article: the global emergence of Helicobacter pylori antibiotic resistance. Aliment Pharmacol Ther. 2016;43:514-33. https://doi.org/10.1111/apt.13625 [DOI:10.1111/apt.13497]
9. Pellicano R, Ribaldone DG, Fagoonee S, Astegiano M, Saracco GM, Mégraud F. A 2016 panorama of Helicobacter pylori infection: key messages for clinicians. Panminerva Med. 2016;58:304-17.
10. Falsafi T, Ehsani A, Niknam V. The role of active efflux in antibiotic - resistance of clinical isolates of Helicobacter pylori. Indian J Med Microbiol. 2009;27:335-40. [DOI:10.4103/0255-0857.55452] [PMID]
11. Abolghasem Tohidpour. CagA-mediated pathogenesis of Helicobacter pylori, Microb Pathog 93 (2016) 44e55. [DOI:10.1016/j.micpath.2016.01.005] [PMID]
12. Megraud FM and Lehours P. "Helicobacter pylori detection and antimicrobial susceptibility testing," Clini Microbiol Rev. 2007; 20(2): 280-322. [DOI:10.1128/CMR.00033-06] [PMID] [PMCID]
13. Jafari F, Aslani M, Shokrzadeh L, Baghai K, Dabiri H, Zojaji H, Razavilar V, Kharaziha P, Haghazali M, Molaei M, Zali MR. Distribution of UreC, CagA, and vacA genes in Helicobacter pylori isolated from patients with gastroduodenal disease in Tehran, Iran. Europ Soc Clin Microbiol Infect Dis 2007. [DOI:10.1016/S0924-8579(07)71682-5]
14. Krashias G, Bashiardes S, Potamitou A, Potamitis GS, Christodoulou Ch. Prevalence of Helicobacter pylori CagA and vacA genes in Cypriot patients. J Infect Dev Ctries 2012; 7(9):642-650. [DOI:10.3855/jidc.2923] [PMID]
15. Hussein NR, Mohammadi M, Talebkhan Y, Doraghi M, Letley DP, Muhammad MK, et al. Differencesin virulence markers between Helicobacter pylori strains from Iraq and those from Iran: potential importance of regional differences in H. pylori-associated disease, Journal of Clinical Microbiology 2008; 46(5): 1774-1779. [DOI:10.1128/JCM.01737-07]
16. Essawi T, Hammoudeh W, Sabri I, Sweidan W, and Farraj M A. Determination of Helicobacter pylori Virulence Genes in Gastric Biopsies by PCR. Hindawi Publishing Corporation ISRN Gastroenterology 2013; 16:119-123. [DOI:10.1155/2013/606258] [PMID] [PMCID]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc