year 14, Issue 5 (September - October 2020)
Iran J Med Microbiol 2020, 14(5): 478-489 |
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Farahmand S, Haeili M, Darban-Sarokhalil D. Molecular Typing and Drug Resistance Patterns of Staphylococcus aureus Isolated From Raw Beef and Chicken Meat Samples. Iran J Med Microbiol 2020; 14 (5) :478-489
URL: http://ijmm.ir/article-1-1134-en.html
URL: http://ijmm.ir/article-1-1134-en.html
1- Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
2- Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran , m.haeili@tabrizu.ac.ir
3- Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2- Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran , m.haeili@tabrizu.ac.ir
3- Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Abstract: (5420 Views)
Background: Staphylococcus aureus is one of the most important food-borne pathogens. The objective of this study was to determine the prevalence, molecular types and drug resistance pattern of S. aureus isolated from retail meat in Tabriz city.
Materials & Methods: 60 raw meat samples (chicken and beef) were taken from different markets and were inoculated in selective Mueller Hinton broth media supplemented with 10% NaCl. Identification of S. aureus isolates was performed using conventional biochemical tests. Susceptibility to different antibiotics and genotypes of isolates were determined by disc diffusion and spa typing methods respectively.
Results: Fifteen S. aureus strains were isolated from 60 different meat samples which belonged to spa types t14870, t3802, t1814, t491, t386, t3424 and spa type t14870 with the frequency of 33.3% was the most prevalent genotype among S. aureus isolates. spa types of three isolates were not found in Ridom Spa Server data base and were considered as novel types. About 46.6% of isolates were resistant to more than one antibiotic and 13.3% of isolates were identified as methicillin resistant S. aureus (MRSA). Tigecycline, imipenem and ceftaroline were found to be the most effective agents against S. aureus isolates.
Conclusion: Oure results revealed a 25% contamination rate with S. aureus. Most of the molecular types of isolates were found to be linked to human infections. High rate of antibiotic resistance was observed among the isolates which poses a great threat to public health.
Materials & Methods: 60 raw meat samples (chicken and beef) were taken from different markets and were inoculated in selective Mueller Hinton broth media supplemented with 10% NaCl. Identification of S. aureus isolates was performed using conventional biochemical tests. Susceptibility to different antibiotics and genotypes of isolates were determined by disc diffusion and spa typing methods respectively.
Results: Fifteen S. aureus strains were isolated from 60 different meat samples which belonged to spa types t14870, t3802, t1814, t491, t386, t3424 and spa type t14870 with the frequency of 33.3% was the most prevalent genotype among S. aureus isolates. spa types of three isolates were not found in Ridom Spa Server data base and were considered as novel types. About 46.6% of isolates were resistant to more than one antibiotic and 13.3% of isolates were identified as methicillin resistant S. aureus (MRSA). Tigecycline, imipenem and ceftaroline were found to be the most effective agents against S. aureus isolates.
Conclusion: Oure results revealed a 25% contamination rate with S. aureus. Most of the molecular types of isolates were found to be linked to human infections. High rate of antibiotic resistance was observed among the isolates which poses a great threat to public health.
Type of Study: Original Research Article |
Subject:
Food Microbiology
Received: 2020/05/7 | Accepted: 2020/08/17 | ePublished: 2020/10/5
Received: 2020/05/7 | Accepted: 2020/08/17 | ePublished: 2020/10/5
References
1. Kadariya J, Smith TC, Thapaliya D. Staphylococcus aureus and staphylococcal food-borne disease: an ongoing challenge in public health. BioMed research international. 2014;2014. [DOI:10.1155/2014/827965] [PMID] [PMCID]
2. Wei-Wei L, Zhu J, Zhen S, Liang X, Jiang Y, Ning L. Analysis of foodborne disease outbreaks in China mainland in 2011. Chin J Food Hygiene. 2018;30:283-8.
3. Jackson CR, Davis JA, Barrett JB. Prevalence and characterization of methicillin-resistant Staphylococcus aureus isolates from retail meat and humans in Georgia. Journal of clinical microbiology. 2013;51:1199-207. [DOI:10.1128/JCM.03166-12] [PMID] [PMCID]
4. McCallum N, Berger-Bächi B, Senn MM. Regulation of antibiotic resistance in Staphylococcus aureus. International Journal of Medical Microbiology. 2010;300:118-29. [DOI:10.1016/j.ijmm.2009.08.015] [PMID]
5. Papadopoulos P, Papadopoulos T, Angelidis AS, Boukouvala E, Zdragas A, Papa A, et al. Prevalence of Staphylococcus aureus and of methicillin-resistant S. aureus (MRSA) along the production chain of dairy products in north-western Greece. Food microbiology. 2018;69:43-50. [DOI:10.1016/j.fm.2017.07.016] [PMID]
6. Wu S, Huang J, Wu Q, Zhang J, Zhang F, Yang X, et al. Staphylococcus aureus isolated from retail meat and meat products in China: incidence, antibiotic resistance and genetic diversity. Frontiers in microbiology. 2018;9:2767. [DOI:10.3389/fmicb.2018.02767] [PMID] [PMCID]
7. Voss A, Loeffen F, Bakker J, Klaassen C, Wulf M. Methicillin-resistant Staphylococcus aureus in pig farming. Emerging infectious diseases. 2005;11:1965. [DOI:10.3201/eid1112.050428] [PMID] [PMCID]
8. Kadlec K, Entorf M, Peters T. Occurrence and characteristics of livestock-associated methicillin-resistant Staphylococcus aureus in quarter milk samples from dairy cows in Germany. Frontiers in microbiology. 2019;10. [DOI:10.3389/fmicb.2019.01295] [PMID] [PMCID]
9. Wang X, Li G, Xia X, Yang B, Xi M, Meng J. Antimicrobial susceptibility and molecular typing of methicillin-resistant Staphylococcus aureus in retail foods in Shaanxi, China. Foodborne pathogens and disease. 2014;11:281-6. [DOI:10.1089/fpd.2013.1643] [PMID]
10. Koreen L, Ramaswamy SV, Graviss EA, Naidich S, Musser JM, Kreiswirth BN. spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro-and macrovariation. Journal of clinical microbiology. 2004;42:792-9. [DOI:10.1128/JCM.42.2.792-799.2004] [PMID] [PMCID]
11. Strommenger B, Braulke C, Heuck D, Schmidt C, Pasemann B, Nübel U, et al. spa typing of Staphylococcus aureus as a frontline tool in epidemiological typing. Journal of clinical microbiology. 2008;46:574-81. [DOI:10.1128/JCM.01599-07] [PMID] [PMCID]
12. Patel JB. Performance standards for antimicrobial susceptibility testing: Clinical and Laboratory Standards Institute; 2017.
13. Founou LL, Founou RC, Essack SY. Antibiotic resistance in the food chain: a developing country-perspective. Frontiers in microbiology. 2016;7:1881. [DOI:10.3389/fmicb.2016.01881] [PMID] [PMCID]
14. Chang Q, Wang W, Regev‐Yochay G, Lipsitch M, Hanage WP. Antibiotics in agriculture and the risk to human health: how worried should we be? Evolutionary applications. 2015;8:240-7. [DOI:10.1111/eva.12185] [PMID] [PMCID]
15. Kluytmans JA, Overdevest IT, Willemsen I, Kluytmans-Van Den Bergh MF, Van Der Zwaluw K, Heck M, et al. Extended-spectrum β-lactamase-producing Escherichia coli from retail chicken meat and humans: comparison of strains, plasmids, resistance genes, and virulence factors. Clinical Infectious Diseases. 2012;56:478-87. [DOI:10.1093/cid/cis929] [PMID]
16. Gharsa H, Slama KB, Lozano C, Gómez-Sanz E, Klibi N, Sallem RB, et al. Prevalence, antibiotic resistance, virulence traits and genetic lineages of Staphylococcus aureus in healthy sheep in Tunisia. Veterinary microbiology. 2012;156:367-73. [DOI:10.1016/j.vetmic.2011.11.009] [PMID]
17. Asokan GV, Vanitha A. WHO global priority pathogens list on antibiotic resistance: an urgent need for action to integrate one health data. Perspectives in public health. 2018;138:87-8. [DOI:10.1177/1757913917743881] [PMID]
18. Ge B, Mukherjee S, Hsu C-H, Davis JA, Tran TTT, Yang Q, et al. MRSA and multidrug-resistant Staphylococcus aureus in US retail meats, 2010-2011. Food microbiology. 2017;62:289-97. [DOI:10.1016/j.fm.2016.10.029] [PMID]
19. Tang Y, Larsen J, Kjeldgaard J, Andersen PS, Skov R, Ingmer H. Methicillin-resistant and-susceptible Staphylococcus aureus from retail meat in Denmark. International journal of food microbiology. 2017;249:72-6. [DOI:10.1016/j.ijfoodmicro.2017.03.001] [PMID]
20. Xing X, Li G, Zhang W, Wang X, Xia X, Yang B, et al. Prevalence, antimicrobial susceptibility, and enterotoxin gene detection of Staphylococcus aureus isolates in ready-to-eat foods in Shaanxi, People's Republic of China. Journal of food protection. 2014;77:331-4. [DOI:10.4315/0362-028X.JFP-13-301] [PMID]
21. Kim YJ, Oh DH, Song BR, Heo EJ, Lim JS, Moon JS, et al. Molecular characterization, antibiotic resistance, and virulence factors of methicillin-resistant Staphylococcus aureus strains isolated from imported and domestic meat in Korea. Foodborne pathogens and disease. 2015;12:390-8. [DOI:10.1089/fpd.2014.1885] [PMID]
22. Narvaez‐Bravo C, Toufeer M, Weese S, Diarra M, Deckert A, Reid‐Smith R, et al. Prevalence of methicillin‐resistant Staphylococcus aureus in Canadian commercial pork processing plants. Journal of applied microbiology. 2016;120:770-80. [DOI:10.1111/jam.13024] [PMID]
23. Alni RH, Mohammadzadeh A, Mahmoodi P. Molecular typing of Staphylococcus aureus of different origins based on the polymorphism of the spa gene: characterization of a novel spa type. 3 Biotech. 2018;8:58. [DOI:10.1007/s13205-017-1061-6] [PMID] [PMCID]
24. Abbasian S, Farahani NN, Mir Z, Alinejad F, Haeili M, Dahmardehei M, et al. Genotypic characterization of Staphylococcus aureus isolated from a burn centre by using agr, spa and SCCmec typing methods. New microbes and new infections. 2018;26:15-9. [DOI:10.1016/j.nmni.2018.08.001] [PMID] [PMCID]
25. Rijnders M, Deurenberg R, Boumans M, Hoogkamp-Korstanje J, Beisser P, Stobberingh E. Population structure of Staphylococcus aureus strains isolated from intensive care unit patients in the Netherlands over an 11-year period (1996 to 2006). Journal of clinical microbiology. 2009;47:4090-5. [DOI:10.1128/JCM.00820-09] [PMID] [PMCID]
26. Hashemizadeh Z, Hadi N, Mohebi S, Kalantar-Neyestanaki D, Bazargani A. Characterization of SCCmec, spa types and Multi Drug Resistant of methicillin-resistant Staphylococcus aureus isolates among inpatients and outpatients in a referral hospital in Shiraz, Iran. BMC research notes. 2019;12:614. [DOI:10.1186/s13104-019-4627-z] [PMID] [PMCID]
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