year 15, Issue 3 (May - Jun 2021)
Iran J Med Microbiol 2021, 15(3): 352-360 |
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Abiri R, Aliabadi M, Kadivarian S, Borji S, Moradi J, Alvandi A. Potentially Probiotic Bacteria Isolated from Preparation Stages of Kermanshahi Traditional Fat. Iran J Med Microbiol 2021; 15 (3) :352-360
URL: http://ijmm.ir/article-1-1121-en.html
URL: http://ijmm.ir/article-1-1121-en.html
Ramin Abiri1 
, Minoo Aliabadi2 , Sepideh Kadivarian2 , Soroush Borji2 , Jale Moradi3 , Amirhooshang Alvandi 
4
, Minoo Aliabadi2 , Sepideh Kadivarian2 , Soroush Borji2 , Jale Moradi3 , Amirhooshang Alvandi 4
1- Fertility and Sterility Research Center, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2- Student Research Committee, and Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
3- Medical Technology Research Center, Research institute for Health Technology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
4- Medical Technology Research Center, Research institute for Health Technology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran ,ah_alvandi@kums.ac.ir
2- Student Research Committee, and Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
3- Medical Technology Research Center, Research institute for Health Technology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
4- Medical Technology Research Center, Research institute for Health Technology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran ,
Abstract: (4911 Views)
Background and Objective: Dairy fermented foods such as yogurt, cheese, fermented milk, butter milk, curd, butter and ghee are used as major diet ingredients in west of IRAN, such as Kermanshah province. Ghee (Kermanshahi traditional oil or roghan-e heiwâni) is traditionally produced from butter milk of yoghurt after fermentation. Review of the literature yielded no study on isolating probiotics from Kermanshahi traditional oil preparation stages. Therefore, purpose of this study was just to focus on isolating and identifying lactic acid bacteria in these products using culture and PCR-sequencing methods.
Materials and Methods: Fifteen samples from each dairy products including yogurt, butter and Kermanshahi traditional oil were collected in Kermanshah province, Iran. Each sample was diluted, homogenized, and cultured on selective medium for growing of lactic acid bacteria. 16SrRNA gene sequences analysis was carried out for final confirmation of these isolates.
Results: After culturing of samples on MRS and M17 under aerobic and anaerobic condition, a total of 78 strains of bacteria were isolated and identified by conventional biochemical tests. The frequency of bacteria in all samples were 48.71% for Lactobacillus, 33.33% for Streptococcus, 6.41% for Enterococcus and 6.41% for Bacillus. Lactobacillus, Streptococcus, Enterococcus, and Bacillus genus, were isolated from 84.44%, 57.78%, 11.11% and 15.56% of all three kind of samples, respectively.
Conclusion: Based on our findings, Lactic acid bacteria and other potentially probiotic microorganisms are present in Kermanshahi traditional oil. Of course the potential probiotic properties of these isolates and impact of consumption of Kermanshahi traditional oil containing those on human health need to be analyzed more. By proving the presence of probiotic bacteria in Kermanshahi oil, it may be falls in the category of functional foods.
Type of Study: Original Research Article |
Subject:
Food Microbiology
Received: 2020/05/2 | Accepted: 2020/12/5 | ePublished: 2021/06/28
Received: 2020/05/2 | Accepted: 2020/12/5 | ePublished: 2021/06/28
References
1. Angmo K, Kumari A, Bhalla TC. Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. LWT-food Sci Technol. 2016;66:428-35. [DOI:10.1016/j.lwt.2015.10.057]
2. Fguiri I, Ziadi M, Atigui M, Ayeb N, Arroum S, Assadi M, et al. Isolation and characterisation of lactic acid bacteria strains from raw camel milk for potential use in the production of fermented Tunisian dairy products. Int J Dairy Technol. 2016;69(1):103-13. [DOI:10.1111/1471-0307.12226]
3. Lim S-M, Im D-S. Screening and characterization of probiotic lactic acid bacteria isolated from Korean fermented foods. J Microbiol Biotechnol. 2009;19(2):178-86. [DOI:10.4014/jmb.0804.269] [PMID]
4. Bhattarai RR, Gautam N, Nawaz MA, Das SKL. Isolation and identification of dominant lactic acid bacteria from dahi: an indigenous dairy product of Nepal Himalayas. J Microbiol, Biotechnol Food Sci. 2016;5(4):358. [DOI:10.15414/jmbfs.2016.5.4.358-363]
5. Tabatabaei Yazdi F, Alizadeh Behbahani B, Mohebbi M, Mortazavi SA, Ghaitaranpour A. Identification of lactic acid bacteria isolated from Tarkhineh, a traditional Iranian fermented food. Sci J Microbiol. 2012;1.
6. Lourens-Hattingh A, Viljoen BC. Yogurt as probiotic carrier food. Int dairy J. 2001;11(1-2):1-17. [DOI:10.1016/S0958-6946(01)00036-X]
7. Adolfsson O, Meydani SN, Russell RM. Yogurt and gut function. Am J Clin Nutr. 2004;80(2):245-56. [DOI:10.1093/ajcn/80.2.245] [PMID]
8. Ghafoorunissa G. Role of trans fatty acids in health and challenges to their reduction in Indian foods. Asia Pacific J Clin Nutr. 2008;17:212-5.
9. Ahmadiasl N, Alipour MR, Andalib S, Ebrahimi H. Effect of ghee oil on blood fat profile and passive avoidance learning in male rats. 2008.
10. Bahrami GR, Rahi H, Pyravi-Vanak Z. Change in fatty acids composition of milk products during the traditional Ghee-making process. J Kerman Uni Med Sci. 2000;7(1):14-9.
11. Ritchie LE, Burke KF, Garcia-Mazcorro JF, Steiner JM, Suchodolski JS. Characterization of fecal microbiota in cats using universal 16S rRNA gene and group-specific primers for Lactobacillus and Bifidobacterium spp. Vet microbiology. 2010;144(1-2):140-6. [DOI:10.1016/j.vetmic.2009.12.045] [PMID]
12. Song D, Ibrahim S, Hayek S. Recent application of probiotics in food and agricultural science. Probiotics. 2012 Oct 3;10:1-34.. [DOI:10.5772/50121]
13. Araújo EA, dos Santos Pires AC, Pinto MS, Jan G, Carvalho AF. Probiotics in dairy fermented products. Rijeja: Intech. 2012 Oct 3:129-48.
14. Narimani, T., Tarinejad, A., Hejazi, M. Isolation and identification of lactic acid bacteria from traditional dairy products of Kleibar, Heris and Varzaghan. Food Hyg, 2013; 3(3 (11)): 23-37.
15. Ebrahimi MT, Ouweh AC, Hejazi MA, Jafari P. Traditional Iranian dairy products: A source of potential probiotic lactobacilli. African J Microbiol Res. 2011;5(1):20-7.
16. Yu J, Wang H, Zha M, Qing Y, Bai N, Ren Y, et al. Molecular identification and quantification of lactic acid bacteria in traditional fermented dairy foods of Russia. J Dairy Sci. 2015;98(8):5143-54. [DOI:10.3168/jds.2015-9460] [PMID]
17. Sadeghi A, Ebrahimi M. Isolation, molecular identification and evaluation of the probiotic properties of dominant Lactobacillus in whole wheat sourdough. J Microb World. 2016 Aug 1;9(2):133-44.
18. Kia Dalirif F, Sadeghi A, Khomeiri M, Kashaninejad M, Aalami M. Evaluating the antimicrobial properties of Lactobacillus brevis isolated from whole barley sourdough. Food Science and Technology. 2018;15(2):247-57.
19. Jamaly N, Benjouad A, Bouksaim M. Probiotic potential of Lactobacillus strains isolated from known popular traditional Moroccan dairy products. Br Microbiol Res J. 2011;1(4):79. [DOI:10.9734/BMRJ/2011/438]
20. Chen T, Wang L, Li Q, Long Y, Lin Y, Yin J, et al. Functional probiotics of lactic acid bacteria from Hu sheep milk. BMC Microbiol. 2020;20(1):1-12. [DOI:10.1186/s12866-020-01920-6] [PMID] [PMCID]
21. Sabir F, Beyatli Y, Cokmus C, Onal‐Darilmaz D. Assessment of potential probiotic properties of Lactobacillus spp., Lactococcus spp., and Pediococcus spp. strains isolated from kefir. J Food Sci. 2010;75(9):M568-M73. [DOI:10.1111/j.1750-3841.2010.01855.x] [PMID]
22. Masuda T, Kimura M, Okada S, Yasui H. Pediococcus pentosaceus Sn26 inhibits IgE production and the occurrence of ovalbumin-induced allergic diarrhea in mice. Biosci, Biotechnol, Biochem. 2010:0912261784-. [DOI:10.1271/bbb.90656] [PMID]
23. Dorri K, Namdar N, HemayatkhahJahromi V. Isolation of lactobacilli from dairy products and their effects on the main pathogenic bacteria in stomach and intestine. Med Lab J. 2013 Apr 10;7(1):22-8.
24. Arqués JL, Rodríguez E, Langa S, Landete JM, Medina M. Antimicrobial activity of lactic acid bacteria in dairy products and gut: effect on pathogens. BioMed Res Int. 2015;2015. [DOI:10.1155/2015/584183] [PMID] [PMCID]
25. Vélez MP, Hermans K, Verhoeven T, Lebeer S, Vanderleyden J, De Keersmaecker S. Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products. J Appl Microbiol. 2007;103(3):666-74. [DOI:10.1111/j.1365-2672.2007.03294.x] [PMID]
26. The isolation of lactic acid bacteria from local dairy products of Gorgan township with the ability to inhibit the growth of some gastrointestinal pathogens. Koohsari H, Rashti Z, Arab Sh. J Food Microbiol. 2019;6(3):22-36.
27. Azat R, Liu Y, Li W, Kayir A, Lin D-b, Zhou W-w, et al. Probiotic properties of lactic acid bacteria isolated from traditionally fermented Xinjiang cheese. J Zhejiang Uni Sci B. 2016;17(8):597-609. [DOI:10.1631/jzus.B1500250] [PMID] [PMCID]
28. Kiran KA, Appaiah KA, Appaiah S. Extension of Shelf Life of Curd--An Indian Fermented Milk by Using A New Isolate of Brevibacillus Brevis Strain as Starter Culture. Innov Rom Food Biotechnol. 2012 Mar 1(10).
29. Najafi T, Eghtesadi S, Rezaei M, Daneshvar K. The effect of Kermanshahi animal oil on serum lipid profile in healthy men. Behbood J. 2010;14(4).
30. Idoui T, Boudjerda J, Leghouchi E, Karam NE. Lactic acid bacteria from" Sheep's Dhan", a traditional butter: Isolation, identification and major technological traits. Grasas Aceites. 2009 Jun 30;60(2):177-83. [DOI:10.3989/gya.085408]
31. Sengun IY, Nielsen DS, Karapinar M, Jakobsen M. Identification of lactic acid bacteria isolated from Tarhana, a traditional Turkish fermented food. Int J food Microbiol. 2009;135(2):105-11. [DOI:10.1016/j.ijfoodmicro.2009.07.033] [PMID]
32. Guarner F, Perdigon G, Corthier G, Salminen S, Koletzko B, Morelli L. Should yoghurt cultures be considered probiotic? Br J Nutr. 2005;93(6):783-6. [DOI:10.1079/BJN20051428] [PMID]
33. Tsai C-C, Chou L-C, Lai S-E, Huang C-C. Effect of cholesterol lowering multiplex lactic acid bacteria on lipid metabolism in a hamster model. African J Microbiol Res. 2016;10(20):708-16. [DOI:10.5897/AJMR2015.7719]
34. MATAR C, VALDEZ JC, MEDINA M, RACHID M, PERDIGON G. Immunomodulating effects of milks fermented by Lactobacillus helveticus and its non-proteolytic variant. J Dairy Res. 2001;68(4):601-9. [DOI:10.1017/S0022029901005143] [PMID]
35. Garsa AK, Kumariya R, Sood SK, Kumar A, Kapila S. Bacteriocin production and different strategies for their recovery and purification. Probiotics Antimicrob Proteins. 2014 Mar 1;6(1):47-58. [DOI:10.1007/s12602-013-9153-z] [PMID]
36. Silva LF, Casella T, Gomes ES, Nogueira MCL, De Dea Lindner J, Penna ALB. Diversity of lactic acid bacteria isolated from Brazilian water buffalo mozzarella cheese. J Food Sci. 2015;80(2):M411-M7. [DOI:10.1111/1750-3841.12771] [PMID]
37. Adnan M, Patel M, Hadi S. Functional and health promoting inherent attributes of Enterococcus hirae F2 as a novel probiotic isolated from the digestive tract of the freshwater fish Catla catla. PeerJ. 2017;5:e3085. [DOI:10.7717/peerj.3085] [PMID] [PMCID]
38. Li B, Zhan M, Evivie SE, Jin D, Zhao L, Chowdhury S, et al. Evaluating the safety of potential probiotic Enterococcus durans KLDS6. 0930 using whole genome sequencing and oral toxicity study. Front Microbiol. 2018;9:1943. [DOI:10.3389/fmicb.2018.01943] [PMID] [PMCID]
39. Hassanzadazar H, Ehsani A, Mardani K. Antibacterial activity of Enterococcus faecium derived from Koopeh cheese against Listeria monocytogenes in probiotic ultra-filtrated cheese. In: Veterinary research forum: an international quarterly journal 2014 (Vol. 5, No. 3, p. 169). Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
40. Torres-Llanez M, Vallejo-Cordoba B, Díaz-Cinco M, Mazorra-Manzano M, González-Córdova A. Characterization of the natural microflora of artisanal Mexican Fresco cheese. Food Control. 2006;17(9):683-90. [DOI:10.1016/j.foodcont.2005.04.004]
41. Beerepoot M, Ter Riet G, Nys S. Lactobacilli versus antibiotics to prevent urinary tract infections: a randomized, double-blind, noninferiority trial in postmenopausal women. Arch Intern Med. 2012;172(9):704-12. [DOI:10.1001/archinternmed.2012.777] [PMID]
42. Bettache G, Fatma A, Miloud H, Mebrouk K. Isolation and identification of lactic acid bacteria from Dhan, a traditional butter and their major technological traits. World Appl Sci J. 2012;17(4):480-8.
43. Franz CM, Huch M, Abriouel H, Holzapfel W, Gálvez A. Enterococci as probiotics and their implications in food safety. Int J Food Microbiol. 2011;151(2):125-40. [DOI:10.1016/j.ijfoodmicro.2011.08.014] [PMID]
44. Franz CM, Stiles ME, Schleifer KH, Holzapfel WH. Enterococci in foods-a conundrum for food safety. Int J Food Microbiol. 2003;88(2-3):105-22. [DOI:10.1016/S0168-1605(03)00174-0]
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