year 13, Issue 4 (September - October 2019)                   Iran J Med Microbiol 2019, 13(4): 302-309 | Back to browse issues page

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Mohammadzadeh R, Mohammadi-Gollou A, Salehabadi Y. The Prevalence of CTX-M Beta-Lactamase Gene in Urinary Tract Infections in patients referring to Bonab city Hospitals. Iran J Med Microbiol 2019; 13 (4) :302-309
1- Department of Biology, Faculty of Basic Sciences, University of Marahgheh, Maragheh, Iran ,
2- Department of Biology, Faculty of Basic Sciences, University of Marahgheh, Maragheh, Iran
3- Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Abstract:   (4296 Views)

Background: Urinary Tract Infection (UTI) is one of the most common bacterial infections, and several studies have reported the prevalence of UTI caused by ESBL-producing E.coli strains in different cities of Iran. The aim of this study was to evaluate antibiotic resistant UTI factors in outpatients and determine the prevalence of ctx-m gene in ESBL-producing E. coli strains of Bonab (East Azerbaijan, Iran).
Materials & Methods: In 2015, 266 clinical isolates of E. coli were collected from clinical laboratories in Bonab. Phenotypic screening and confirmation tests for extended-spectrum beta-lactamases (ESBLs) were carried out using disk diffusion (Kirby Bauer) method. All of the ESBL producing isolates were tested by PCR using specific primers. A total of 10 PCR products were randomly selected and purified and subsequently sent for sequencing.
Results & Discussion: The maximum resistance was seen regarding ampicillin (67.3 %) and the maximum sensitivity was seen regarding imipenem (92.5%). In this study, 45 % of isolates were multidrug resistance, which showed at least resistance for three antibiotics. Out of 154 isolates, 58 (37.7%) isolates were ESBL producers which 94.8% of isolates contained ctx-m gene. The prevalence of the ctx-m gene, as in other parts of Iran and the world, is high in Bonab. For the treatment of infections suspected to be an ESBL producer, appropriate antibiotics should be selected, and strains that have decreased their sensitivity to ceftazidime, cefotaxime, and cefetoxin should be considered for ESBL genes.

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Type of Study: Brief Original Article | Subject: Molecular Epidemiology
Received: 2019/06/25 | Accepted: 2019/09/11 | ePublished: 2019/12/31

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