year 13, Issue 1 (March - April 2019)                   Iran J Med Microbiol 2019, 13(1): 69-79 | Back to browse issues page

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Kalteh S, Ojagh S M, Tabarrayi A, Zolfaghari M. Investigating the Possibility of the Listeria Monocytogenes Entering Into a Viable But Non-Culturable (VBNC) Form and Expression of the Pathogenic Genes During the Frozen Storage of (-18ºC) Rainbow Trout Fish Nugget. Iran J Med Microbiol. 2019; 13 (1) :69-79
1- Department of Fishieries, Faculty of Fishieries and Environment, University of Agricultural Sciences and Natual Resources of Gorgan, Gorgan, Iran
2- Department of Fishieries, Faculty of Fishieries and Environment, University of Agricultural Sciences and Natual Resources of Gorgan, Gorgan, Iran ,
3- Department of Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
Abstract:   (4559 Views)
Background and Aims: The purpose of this study was to investigate the possibility of Listeria monocytogenes entering the VBNC state during the frozen storage and the expression of its pathogenic genes
Materials and Methods: Bacteria in 106 Colony counts of in mid log phase were inoculated into three Culture medium including Normal Saline (NS), BHI Broth and Fish Broth (FB) and kept at -18ºCfor 2 months and examined. Then, bacteria were evaluated on enriched medium BHI agar using culture methods (colony count) over times 4 and 8 hours, 2, 4, 8, 20, 30 and 60 days after the freezing shock using the method of RT-PCR for investigating the expression of 16S rRNA, hly and inlA genes; they were evaluated before and after the freezing shock.
Results: This bacterium retained its ability to cultivate until the end of the shock, but reduced its number. Freezing stopped the expression of genes of hly and inlA, as these genes were not expressed in a rich culture medium either. By adding blood to the rich culture medium of this bacterium, only the hemolysin O pathogen gene was expressed.
Conclusion: Although freezing does not lead to the introduction of this bacterium into the VBNC state, it is effective as an adverse environmental factor for the bacteria in the expression of its pathogenic genes. Blood and its agents can act as an agent for the induction and clarification of the hly gene, and the expression of pathogenic bacterial genes are independent of each other.
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Type of Study: Original Research Article | Subject: Food Microbiology
Received: 2019/01/12 | Accepted: 2019/05/22 | ePublished: 2019/07/22

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