year 10, Issue 6 (January - February 2017)                   Iran J Med Microbiol 2017, 10(6): 44-51 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khanjari A, Jahanbakhsh A, Rajaie P, Fayazfar S, Akhondzadeh Basti A, Esmaeili H, et al . Antimicrobial activity of poly lactic acid films incorporated with Trachyspermum ammi essential oil and ethanolic extract of propolis on the growth of some bacterial foodborne pathogens. Iran J Med Microbiol 2017; 10 (6) :44-51
URL: http://ijmm.ir/article-1-543-en.html
1- Department of Food hygiene, Faculty of Veterinary medicine, University of Tehran, Tehran, Iran , khanjari@ut.ac.ir
2- Department of Food Science, Faculty of Agriculture, Islamic Azad University, Varamin Branch, Varamin, Iran
3- Department of Food hygiene, Faculty of Veterinary medicine, University of Tehran, Tehran, Iran
4- Department of Microbiology, Faculty of Veterinary medicine, University of Tehran, Tehran, Iran
5- Department of Poultry disease, Faculty of Veterinary medicine, University of Tehran, Tehran, Iran
6- Department of Food Science, Faculty of Agriculture, Islamic Azad University, Savadkooh Branch, Savadkooh, Iran
Abstract:   (11429 Views)

Background and Aim: Using active antimicrobial packaging reduce the risks of growth of pathogenic or spoilage microorganisms in foods. In this study, antimicrobial activity of poly lactic acid (PLA) films containing different concentrations (0, 0.5, 1 and 1.5%) of Trachyspermum ammi essential oil and ethanolic extract of propolis (0, 1 and 2%) was evaluated against Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7 and vibrio parahaemolyticus by using disk diffusion assay.

Materials and Methods: Circular discs of poly lactic acid films incorporated by essential oil and ethanolic extract of propolis, prepared by casting method on glass petri dishes, were placed on Muller-Hinton agar plates that previously inoculated by tested bacteria. Diameters of inhibition zones were measured after 24 h incubation of plates at 35o C, by using Digital Caliper and Digimizer software.

Results: Result of this study showed that the inhibition zone was increased with increasing concentration of essential oil for all tested bacteria. Also, gram positive bacteria were more sensitive to the poly lactic acid films containing essential oil than gram negative bacteria. The results revealed that L. monocytogenes was the most sensitive bacteria against films containing Trachyspermum ammi essential oil alone or in combination by ethanolic extract of propolis. Also, poly lactic acid films containing ethanolic extract of propolis showed no inhibitory effects against all tested bacteria.

Conclusions: Poly lactic acid films containing Trachyspermum ammi essential oil have a high potential for antimicrobial food packaging applications to enhance the safety of food products.

Full-Text [PDF 682 kb]   (2309 Downloads)    
Type of Study: Original Research Article | Subject: Food Microbiology
Received: 2016/04/10 | Accepted: 2016/06/21 | ePublished: 2016/10/17

References
1. Khanjari A, Misaghi A, Basti AA, Esmaeili H, Cherghi N, Partovi R, Mohammadian MR, Choobkar N. Effects of Zataria multiflora Boiss. Essential Oil, Nisin, pH and Temperature on Vibrio parahaemolyticus ATCC 43996 and Its Thermostable Direct Hemolysin Production. J Food Safety 2013;33(3):340-7. [Article]
2. Tavakoli HR, Mashak Z, Moradi B, Sodagari HR. Antimicrobial activities of the combined use of Cuminum cyminum L. essential oil, nisin and storage temperature against Salmonella Typhimurium and Staphylococcus aureus in vitro. Jundishapur J Microbiol 2015;8(4).e24838. [in persion] [PubMed]
3. Emiroğlu ZK, Yemiş GP, Coşkun BK, Candoğan K. Antimicrobial activity of soy edible films incorporated with thyme and oregano essential oils on fresh ground beef patties. Meat Sci. 2010;86(2):283-8. [Article]
4. Salmieri S, Islam F, Khan RA, Hossain FM, Ibrahim HM, Miao C, Hamad WY, Lacroix M. Antimicrobial nanocomposite films made of poly (lactic acid)–cellulose nanocrystals (PLA–CNC) in food applications—part B: effect of oregano essential oil release on the inactivation of Listeria monocytogenes in mixed vegetables. Cellulose 2014; 21(6):4271-85. [Article]
5. Du WX, Olsen CW, Avena‐Bustillos RJ, McHugh TH, Levin CE, Mandrell R, Friedman M. Antibacterial Effects of Allspice, Garlic, and Oregano Essential Oils in Tomato Films Determined by Overlay and Vapor‐Phase Methods. J Food Sci 2009;74(7):390-7. [PubMed]
6. Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. Int J food Microbiol 2004; 94(3):223-53. [PubMed]
7. Ashrafi Tamai I, Zahraei Salehi T, Khosravi AR, Sharifzadeh A, Balal A. Chemical composition and anti-candida activity of Trachyspermum ammi essential oil on azoles resistant Candida albicans isolates from oral cavity of HIV+ patients. J Med Plants 2013; 2(46):137-49. [Article]
8. Khosravi AR, Shokri H, Sohrabi N. Potential effects of Trachyspermum copticum essential oil and propolis alcoholic extract on Mep3 gene expression of Microsporum canis isolates. J Mycol Med 2014; 24(3):101-7. [PubMed]
9. Zargari A. Medicinal plants. Tehran University Publications. ISBN; 1995 in [in Persian].
10. Mahboubi M, Kazempour N. Chemical composition and antimicrobial activity of Satureja hortensis and Trachyspermum copticum essential oil. Iranian J Microbiol 2011; 3(4):194-200 [in Persian]. [PubMed]
11. Abdulkhani A, Hosseinzadeh J, Ashori A, Esmaeeli H. Evaluation of the antibacterial activity of cellulose nanofibers/polylactic acid composites coated with ethanolic extract of propolis. Polym Composite 2017;38 (1): 13-19 [Article]
12. Erdohan ZÖ, Çam B, Turhan KN. Characterization of antimicrobial polylactic acid based films. J Food Eng 2013; 119(2):308-15. [Article]
13. Soleymani N, Sattari M, Sepehriseresht S, Daneshmandi S. Evaluation of reciprocal pharmaceutical effects and antibacterial activity of Bunium persicum essential oil against some Gram positive and Gram negative bacteria. Iranian J Med Microbiol 2010; 4(1):26-34. [in Persian] [Article]
14. Nori MP, Favaro-Trindade CS, de Alencar SM, Thomazini M, de Camargo Balieiro JC, Castillo CJ. Microencapsulation of propolis extract by complex coacervation. LWT-Food Sci Technol 2011; 44(2):429-35. [Article]
15. González A, Igarzabal CI. Soy protein–Poly (lactic acid) bilayer films as biodegradable material for active food packaging. Food Hydrocolloids 2013; 33(2):289-96. [Article]
16. Ekhtiarzadeh H, Akhondzadeh Basti A, Misaghi A, Sari A, Khanjari A, Rokni N, Abbaszadeh S, Partovi R. Growth response of Vibrio parahaemolyticus and Listeria monocytogenes in salted fish fillets as affected by Zataria multiflora Boiss. essential oil, nisin, and their combination. J Food Safety 2012; 32(3):263-9. [Article]
17. Shakeri MS, Shahidi F, Beiraghi‐Toosi S, Bahrami A. Antimicrobial activity of Zataria multiflora Boiss. essential oil incorporated with whey protein based films on pathogenic and probiotic bacteria. Int J Food Sci Tech 2011; 46(3):549-54. [Article]
18. Mehdizadeh T, Tajik H, Rohani SM, Oromiehie AR. Antibacterial, antioxidant and optical properties of edible starch-chitosan composite film containing Thymus kotschyanus essential oil. VRF. 2012; 3 (3), 167-173. [PubMed]
19. Akhila A, editor. Essential oil-bearing grasses: the genus Cymbopogon. CRC Press; 2009.
20. Jahani S, Kavoosi G, Shakiba A. Chemical and Biological Properties of Trachyspermum ammi Encapsulated in Gelatin Nanofilms. Int J Infect 2014; 1(1).e 14820 [Article]
21. Nikaido, H., 1994. Prevention of drug access to bacterial targets: Permeability barriers and active efflux. Sci; 264, 382–388. [PubMed]
22. Silici S, Kutluca S. Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J Ethnopharmacol 2005;99(1):69-73. [PubMed]
23. Uzel A, Önçağ Ö, Çoğulu D, Gençay Ö. Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiol Res 2005;160(2):189-95. [PubMed]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc