year 10, Issue 5 (November - December 2016)                   Iran J Med Microbiol 2016, 10(5): 45-51 | Back to browse issues page

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Yegan mohammadi M, Khanjari A, Akhondzadeh Basti A, Bokaie S, Cheraghi N, Fayazfar S, et al . Evaluation of the antimicrobial effect of chitosan and whey proteins isolate films containing free and nanoliposomal garlic essential oils against Listeria monocytegenes, E.coli O157:H7 and Staphylococcus aureus. Iran J Med Microbiol. 2016; 10 (5) :45-51
1- Department of Food Hygiene, Faculty of Veterinary medicine, University of Tehran, Tehran, Iran
2- Department of Food Hygiene, Faculty of Veterinary medicine, University of Tehran, Tehran, Iran ,
Abstract:   (10052 Views)

Background and Aim: Active antimicrobial packaging is an innovative technique that can enhance safety and shelf life of foods. In this study antimicrobial activity of chitosan and whey protein isolate (WPI) films incorporated with free and nano-liposomal garlic essential oil was investigated against Listeria monocytogenes, Escherichia coli O157:H7 and Staphylococcus aureus.

Materials and Methods: This study was done in 2015 and disk diffusion method was applied to determine antimicrobial effect of films. Films were cut into circular disks with 9 mm diameter and put on the inoculated BHI agar plates with tested microorganisms. Then plates incubated for 24 h at 37oC. The diameter of inhibition zone was measured by digital caliper. The statistical analysis was done by one-way analysis of variance (ANOVA) followed by Tukey's test.

Results: The results of this study revealed pure chitosan and WPI films alone or incorporated with nano-liposomal garlic essential oil did not show any inhibitory effects on tested microorganisms. Incorporation of 2% or higher concentrations of garlic essential oil to the chitosan solution showed the antibacterial activity of films against all tested microorganisms, whereas when the WPI solution incorporated with 3% or higher concentrations of garlic essential oil the antibacterial activity films was seen against all tested microorganisms. Also the results revealed that S. aureus and L. monocytogenes were more sensitive to chitosan and WPI films incorporated with garlic essential oil.

Conclusions: Our results declared that the films incorporated with garlic essential oil have the potential to be used as an active antimicrobial packaging.

Full-Text [PDF 633 kb]   (3406 Downloads)    
Type of Study: Original Research Article | Subject: Food Microbiology
Received: 2015/10/11 | Accepted: 2016/09/10 | ePublished: 2016/10/16

1. Makwana S, Choudhary R, Dogra N, Kohli P, Haddock J. Nanoencapsulation and immobilization of cinnamaldehyde for developing antimicrobial food packaging material. LWT-Food Sci Technol 2014;57 (2):470-6. [Article]
2. CDC Estimates of Foodborne Illness in the United States. (2012). Centers for disease control and prevention. Retrieved April 14, 2012, from foodborneburden/2011-foodborne-estimates.html
3. Günlü A, Koyun E. Effects of vacuum packaging and wrapping with chitosan based edible film on the extension of the shelf life of sea bass (Dicentrarchus labrax) fillets in cold storage (4oC). Food Bioprocess Tech 2013;6 (7):1713-9. [Article]
4. Salmieri S, Islam F, Khan RA, Hossain FM, Ibrahim HM, Miao C, Hamad WY, Lacroix M. Antimicrobial nanocomposite films made of poly (lactic acid)–cellulose nanocrystals (PLA–CNC) in food applications—part B: effect of oregano essential oil release on the inactivation of Listeria monocytogenes in mixed vegetables. Cellulose 2014;21 (6):4271-75. [Article]
5. Mehdizadeh T, Tajik H, RazaviRohani SM, Oromiehie, AR. Antibacterial, antioxidant and optical properties of edible starch-chitosan composite film containing Thymus kotschyanus essential oil. Vet Res Forum 2012; 3(3): 167 – 73. [in persion] [PubMed]
6. Moradi M, Tajik H, Razavi Rohani SM, Oromiehie AR. Effectiveness of Zataria multiflora Boiss essential oil and grape seed extract impregnated chitosan film on ready‐to‐eat mortadella‐type sausages during refrigerated storage. J Sci Food Agric 2011;91 (15):2850-7. [in persion] [PubMed]
7. Ruiz-Navajas Y, Viuda-Martos M, Sendra E, Perez-Alvarez JA, Fernández-López J. In vitro antibacterial and antioxidant properties of chitosan edible films incorporated with Thymus moroderi or Thymus piperella essential oils. Food Control 2013;30 (2):386-392. [Article]
8. Seydim, AC, Sarikus G. Antimicrobial activity of whey protein based edible films incorporated with oregano, rosemary and garlic essential oils. Food Res Int 2006;39 (5): 639–44. [Article]
9. Shakeri MS, Shahidi F, Beiraghi‐Toosi S, Bahrami A. Antimicrobial activity of Zataria multiflora Boiss. essential oil incorporated with whey protein based films on pathogenic and probiotic bacteria. Int J Food Sci Tech 2011;46 (3):549-54. [in persion] [Article]
10. Basti AA, Mashak Z, Khanjari A, Rezaei A, Mohammadkhan F, Taheri MA, Faghih Fard P, Tayar N. The study on the effects of garlic Essential oil on growth curve and TDH toxin production of Vibrio parahaemolyticus. JMP 2014;2 (50):156-62. [in persion] [Article]
11. Chand B. Antibacterial effect of garlic (AlliumSativum) and Ginger (ZingiberOfficinale) against Staphylococcus aureus, Salmonella Typhi, Escherichia Coli and Bacillus Cereus. J Microb Biotech Food Sci 2013;2 (4):2481-91.
12. Gortzi O,Lalas S, Chinou I, Tsaknis J. Evaluation of the Antimicrobial and Antioxidant Activities of Origanum dictamnus Extracts before and after Encapsulation in Liposomes, Molecule. 2007;12 (5): 932-45 [PubMed]
13. Liolios SS, Gortzi O, Lalas S, Tsaknis J, Chinou I. Liposomal incorporation of carvacrol and thymol isolated from the essential oil of Origanum dictamnus L. and in vitro antimicrobial activity. Food Chem 2009;112 (1):77–83 [Article]
14. Mozafari MR, Khosravi-Darani K, Borazan GG, Cui J, Pardakhty A, Yurdugul S. Encapsulation of food ingredients using nanoliposome technology, Int J Food Prop 2008;11 (4):833-44. [Article]
15. Sebaaly C, Jraij A, Fessi H, Charcosset C, Greige-Gerges H. Preparation and characterization of clove essential oil-loaded liposomes. Food chem 2015;178:52-62. [Article]
16. Jaafar-Maalej C, Roudayna D, Veronique A, Abdelhamid E, Hatem F. Ethanol injection method for hydrophilic and lipophilic drug-loaded liposome preparation. J Liposome Res 2010; 20 (3):228–43 [PubMed]
17. Pranoto Y, Rakshit SK, Salokhe VM. Enhancing antimicrobial activity of chitosan films by incorporating garlic oil, potassium sorbate and nisin. LWT - Food Sci Technol 2005;38 (8): 859-65. [Article]
18. Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 2004;94 (3):223-53.
19. Ghanbarzadeh B, Almasi H. Physical properties of edible emulsified films based on carboxymethyl cellulose and oleic acid. Int J Biol Macromol 2011;48 (1): 44-49. [in persion] [Article]
20. Soleymani N, Sattari M, Sepehriseresht S, Daneshmandi S, Derakhshan. Evaluation of reciprocal pharmaceutical effects and antibacterial activity of Bunium persicum essential oil against some Gram positive and Gram negative bacteria. Iran J Med Microbiol 2010;4 (1):26-34. [in persion] [Article]
21. Du WX, Avena-Bustillos RJ, Hua SST, McHugh TH. Antimicrobial volatile essential oils in edible films for food safety. Science against microbial pathogens: communicating current research and technological advances”, A. Mendez-Vilas (ed.) 2011; 1124-34. [Article]
22. Tavakoli HR, Mashak Z, Moradi B, Sodagari HR. Antimicrobial activities of the combined use of Cuminum cyminum L. essential oil, nisin and storage temperature against Salmonella Typhimurium and Staphylococcus aureus in vitro. JJM 2015;8 (4) e24838. [in persion] [PubMed]

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