Evaluation of a New Colorimetric Medium for Detection of Extended Spectrum β-lactamases (ESBLs) Producing Isolates of Entrobacteriaceae family

Shahsavari, Shirin; Salmanzade Ahrabi, Siavosh; Falsafi, Tahereh; Irajian, Gholam Reza

year 10, Issue 2 (May - June 2016) Iran J Med Microbiol 2016, 10(2): 75-82 | Back to browse issues page

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Shahsavari S, Salmanzade Ahrabi S, Falsafi T, Irajian G R. Evaluation of a New Colorimetric Medium for Detection of Extended Spectrum β-lactamases (ESBLs) Producing Isolates of Entrobacteriaceae family. Iran J Med Microbiol 2016; 10 (2) :75-82
URL: http://ijmm.ir/article-1-422-en.html

Evaluation of a New Colorimetric Medium for Detection of Extended Spectrum β-lactamases (ESBLs) Producing Isolates of Entrobacteriaceae family

Shirin Shahsavari

¹, Siavosh Salmanzade Ahrabi²

, Tahereh Falsafi²

, Gholam Reza Irajian³

1- Biotechnology Department, Faculty of Biology Sciences, Alzahra University, Tehran, Iran , shirin_sh777@yahoo.com
2- Microiology Department, Faculty of Sciences, Alzahra University, Tehran, Iran
3- Medical Microbiology Department, Iran University of Medical Sciences, Tehran, Iran

Abstract: (9060 Views)

Background and Aim: Due to the increased growth of bacteria producing ESBL, among the members of the Enterobacteriaceae family, monitoring and treatment of nosocomial infections are important, especially caused by ESBL-producing clinical isolates belonging to this family. The aim of this study is to evaluate a rapid method, using new colorimetric culture was made at the University of Al-Zahra (Tehran, Iran).The used method to detect ESBLs, according to disk diffusion method based on CLSI on colorimetric culture and the results of it, compared with the Müller-Hinton agar for disk diffusion method.

Materials and Methods: In this study, the clinically isolated samples belonged to the Enterobacteriaceae family which was evaluated in both Müller-Hinton agar medium and colorimetric culture using ceftazidime (CAZ), ceftazidime + clavulanate (CZA), cefotaxime + clavulanate (CTC) and Cefotaxime (CTX) discs.

Results & Conclusions: Because of the metabolic activity of bacteria, a color change occurred from red to yellow in colorimetric culture after 5-6 hours and red inhibitory zone created around the disc. The obtained results from the colorimetric method are same with the results of Müller-Hinton agar method for disk diffusion after a day.

Keywords: Colorimetric medium, ESBLs, Rapid detections, Disk diffusion test

Full-Text [PDF 831 kb] (4628 Downloads)

Type of Study: Brief Original Article | Subject: Medical Bacteriology
Received: 2015/05/4 | Accepted: 2016/02/25 | ePublished: 2016/07/24

References

1. Bush K, Fisher JF. Epidemiological expansion, structural studies, and clinical challenges of new β-lactamases from Gram-negative bacteria. Annu Rev Microbiol. 2011 (65):455-78. [PubMed]

2. Zahar JR, Lortholary O, Martin C, Potel G, Plesiat P, Nordmann P. Addressing the challenge of extended-spectrum β-lactamases. Curr Opin Investig Drugs 2009 Feb (10):172-80. [PubMed]

3. Carattoli A. Resistance plasmid families in Enterobacteriaceae. Antimicrob Agents Chemother. 2009 Jun (53):2227-38. [Article]

4. Pitout JD, Laupland KB. Extended-spectrum β-lactamase-producing Enterobacteriaceae: an emerging public-health concern. Lancet Infect. Dis 2008 Mar (8):159–66. [PubMed]

5. Chaudhary U, Agarwal R. Extended spectrum-lactamases (ESBL) - an emerging threat to clinical therapeutics. Indian J Med Microbiol 2004 Apr-Jun (22): 75–80. [PubMed]

6. Paterson DL, Bonomo RA. Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev 2005 Oct (18): 657–86. [PubMed]

7. Giske CG, Sundsfjord AS, Kahlmeter G, Woodford N, Nordmann P, Paterson DL, et al. Redefining extended-spectrum beta-lactamases: Balancing science and clinical need. J Antimicrob Chemother 2009 Jan (63):1–4. [PubMed]

8. Bush K, Jacoby GA. Updated functional classification of β-lactamases. Antimicrob Agents Chemother 2010 Mar (54):969 –976. [PubMed]

9. Chaudhary U, Aggarwal R. Extended Spectrum β-Lactamases-An Emerging threat to Clinical therapeutics. Indian J Med Microbiol. 2004 Apr-Jun (22):75–80. [PubMed]

10. Schwaber MJ, Navon-Venezia S, Kaye KS, Ben-Ami R, Schwartz D, Carmeli Y. Clinical and economic impact of bacteremia with extended-spectrum β-lactamase-producing Enterobacteriaceae. Antimicrob Agents Chemother 2006 Apr (50):1257-62. [PubMed]

11. Kluytmans-Vandenberg MF, Kluytmans JA, Voss A. Dutch guideline for preventing nosocomial transmission of highly resistantmicro-organisms (HRMO). Infection 2005 Oct (33):309–313. [PubMed]

12. Eric S, Sanack B, Kocagoz T, Kocagoz S, Hascelik G. Rapid 4 to 6 hour detection of extended-spectrum beta-lactamases in a routine laboratory. Scand J Infect Dis 2007 (39): 781_785. [PubMed]

13. Kocagoz T, Ercis S, Darka O, Salmanzadeh-Ahrabi S, Kocagoz S, Hasc¸elik G. Quicolor: a novel system for rapidantibacterial susceptibility testing. Ann Microbiol 2007 (57):131_135. [Article]

14. Bolandghamatpoor Z, Abdi-Ali A, Salmanzadeh Ahrabi S. Design and Evaluation Rapid Antibacterial Susceptibility Testing for Enterobacteriaceae. Journal of Applied Biologiy of Alzahra University (Tehran, Iran) 2014 Winter (26):1-8.

15. Sajed R. Optimization of the colorimetric medium for antibacterial susceptibility test. M.Sc.Thesis. Alzahra University (Tehran, Iran) 2013.

16. Clinical and Laboratory Standards Institute (formerly NCCLS). Performance Standards for Antimicrobial susceptibilityTesting

17. Clinical and Laboratory Standards Institute (formerly NCCLS). Performance Standards for Antimicrobial susceptibilityTesting. 15th Informational Supplement M100-S23 CLSI, Wayne, PA, USA 2013. [Article]

18. Ho PL, Ho AY, Chow KH, Wong RC, Duan RS, Ho WL and et al. Occurrence and molecular analysis of extendedspectrumbeta-lactamase-producing Proteus mirabilis inHong Kong, 1999_2002. J Antimicrob Chemother 2005 (55):/840_45. [Article]

19. Linscott AJ, Brown WJ. Evaluation of 4 commercially available extended-spectrum beta-lactamase phenotypic confirmation tests. J Clin Microbiol 2005 (43):1081_1085. [Article]