Evaluation of a New Colorimetric Medium for Detection of Extended Spectrum β-lactamases (ESBLs) Producing Isolates of Entrobacteriaceae family

Shahsavari, Shirin; Salmanzade Ahrabi, Siavosh; Falsafi, Tahereh; Irajian, Gholam Reza

year 10, Issue 2 (May - June 2016) Iran J Med Microbiol 2016, 10(2): 75-82 | Back to browse issues page

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Shahsavari S, Salmanzade Ahrabi S, Falsafi T, Irajian G R. Evaluation of a New Colorimetric Medium for Detection of Extended Spectrum β-lactamases (ESBLs) Producing Isolates of Entrobacteriaceae family. Iran J Med Microbiol 2016; 10 (2) :75-82
URL: http://ijmm.ir/article-1-422-en.html

Evaluation of a New Colorimetric Medium for Detection of Extended Spectrum β-lactamases (ESBLs) Producing Isolates of Entrobacteriaceae family

Shirin Shahsavari¹

, Siavosh Salmanzade Ahrabi²

, Tahereh Falsafi²

, Gholam Reza Irajian³

1- Biotechnology Department, Faculty of Biology Sciences, Alzahra University, Tehran, Iran , shirin_sh777@yahoo.com
2- Microiology Department, Faculty of Sciences, Alzahra University, Tehran, Iran
3- Medical Microbiology Department, Iran University of Medical Sciences, Tehran, Iran

Abstract: (9986 Views)

Background and Aim: Due to the increased growth of bacteria producing ESBL, among the members of the Enterobacteriaceae family, monitoring and treatment of nosocomial infections are important, especially caused by ESBL-producing clinical isolates belonging to this family. The aim of this study is to evaluate a rapid method, using new colorimetric culture was made at the University of Al-Zahra (Tehran, Iran).The used method to detect ESBLs, according to disk diffusion method based on CLSI on colorimetric culture and the results of it, compared with the Müller-Hinton agar for disk diffusion method.

Materials and Methods: In this study, the clinically isolated samples belonged to the Enterobacteriaceae family which was evaluated in both Müller-Hinton agar medium and colorimetric culture using ceftazidime (CAZ), ceftazidime + clavulanate (CZA), cefotaxime + clavulanate (CTC) and Cefotaxime (CTX) discs.

Results & Conclusions: Because of the metabolic activity of bacteria, a color change occurred from red to yellow in colorimetric culture after 5-6 hours and red inhibitory zone created around the disc. The obtained results from the colorimetric method are same with the results of Müller-Hinton agar method for disk diffusion after a day.

Keywords: Colorimetric medium, ESBLs, Rapid detections, Disk diffusion test

Full-Text [PDF 831 kb] (5004 Downloads)

Type of Study: Brief Original Article | Subject: Medical Bacteriology
Received: 2015/05/4 | Accepted: 2016/02/25 | ePublished: 2016/07/24

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