year 11, Issue 2 (May - June 2017)                   Iran J Med Microbiol 2017, 11(2): 9-16 | Back to browse issues page

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Mohsenpoor M, Mehrabi Tavana A, Ataee R, Ranjbar R, Mirnejad R. Isolation and Identification of Streptococcus pneumoniae from Admitted Patients in Tehran Hospitals in during 2013-2015. Iran J Med Microbiol. 2017; 11 (2) :9-16
1- Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
2- Research Centre for Health Management, Baqiyatallah University of Medical Sciences, Tehran, Iran ,
3- Health and Nutrition Research, Baqiyatallah University of Medical Sciences, Tehran, Iran
4- Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (9222 Views)

Background and Aim: Streptococcus pneumoniae is Gram-positive bacterium that causes pneumonia, septicemia, meningitis, otitis media, sinusitis, bronchitis, bacteremia and infectious processes is introduced. Due to the clinical importance of this bacterium and correct and timely diagnosis and separation of isolated samples from patients, this study were performed to identify and isolate strains of Pneumococcus from clinical samples by culture and PCR methods.

Materials and Methods: In this study, 175 various clinical samples were collected from admitted patients in Tehran hospitals during 3 years (2013-2015),and culture and PCR methods used to isolate and identify of strains of Pneumococcus (after genome extraction and in the presence of specific primer) beside the standard strain ATCC 49619 (as control).

Results: In this study, 40 strains of Pneumococcus have been identified and approved by culture and PCR methods. Amplified amplicon in this study, was a 160 bp fragment that were found in all strains of Streptococcus pneumoniae. Also, the PCR product was confirmed by sequencing.

Conclusions: According to the obtained results in this study, PCR similar to culture, is a reliable method, but has been assessed relatively faster that can be used in the future to replace the old and conventional methods (biochemical tests and culture).

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Type of Study: Original | Subject: Medical Bacteriology
Received: 2015/02/8 | Accepted: 2016/10/4 | ePublished: 2016/10/16

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